1
Introduction
Overview
Introduction
The iPrep
™
Purification System consists of the iPrep
™
Purification Instrument,
iPrep
™
Protocol Cards, and iPrep
™
Kits that allow automated, fast, and reliable
nucleic acid purification from various samples within 20-30 minutes
(depending on the type of iPrep
™
Kit used).
The iPrep
™
Purification Instrument is based on the patented Magtration
®
(magnetic filtration) technology from PSS (Precision System Science) which
traps magnetic beads against the sidewall of the pipetting tip. This improves
the washing of beads and DNA recovery compared to other magnetic based
purification systems that trap magnetic beads on the bottom of the reaction
well.
The iPrep
™
Purification System eliminates filtration and centrifugation from
nucleic acid purification. The purified DNA is suitable for use in various
downstream applications including PCR, STR (short-tandem repeat analysis),
and restriction enzyme digestion.
See page 5 for details on various parts of the system.
System
Components
The iPrep
™
Purification system consists of:
•
iPrep
™
Purification Instrument
The iPrep
™
Purification Instrument allows automated purification of
nucleic acids from up to 13 samples (12 s 1 positive control) at a
time using magnetic bead based technology. The instrument is a simple,
user friendly unit with small footprint and is controlled by the iPrep
™
Protocol Card. See page 5 for details.
•
iPrep
™
Kits
The iPrep
™
Kits contain the sample preparation reagents as well as
reagents (buffers for the purification protocol and magnetic beads) and
plastic disposables for nucleic acid purification from a variety of samples
using magnetic bead based technology. See the manual supplied with each
kit for details.
•
iPrep
™
Protocol Card
To use each iPrep
™
Kit with the iPrep
™
Instrument, you need to purchase a
protocol specific iPrep
™
Protocol Card from Invitrogen. Each iPrep
™
Protocol Card is pre-programmed with the purification protocol that
directs the volume of reagents used and incubation time.
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