Guidelines for estimating run time
• Refer to the E
‑
Gel
™
Sizing DNA Ladder migration pattern table to estimate target DNA run time to
the reference line.
• The E
‑
Gel
™
DNA Sizing Ladder is also used as a size reference marker. Refer to the NGS library
size selection reference to estimate run time from the reference line to the collection well.
• The run times indicated in the table are estimates. Monitor your gel in real time during the run to
ensure the sample does not pass the recovery well.
• Identically sized bands in different wells may migrate differently.
• DNA fragment size, amount, and salt content can affect migration rates.
Table 4 E
‑
Gel
™
sizing DNA ladder migration pattern
Ladder
Fragment size
DNA amount (per 25 µL)
Migration time to
reference line
1,500 bp
1.5 ng
∼
19.5 min
1,200 bp
1.5 ng
∼
18.5 min
1,000 bp
6.0 ng
∼
17.5 min
900 bp
2.0 ng
∼
17 min
800 bp
2.0 ng
∼
16.5 min
700 bp
2.0 ng
∼
16 min
600 bp
2.0 ng
∼
15.5 min
500 bp
6.0 ng
∼
14.5 min
450 bp
2.0 ng
∼
14 min
400 bp
2.0 ng
∼
13.5 min
350 bp
2.0 ng
∼
13 min
300 bp
2.0 ng
∼
12.5 min
250 bp
2.0 ng
∼
11.5 min
200 bp
6.0 ng
∼
11 min
150 bp
2.0 ng
∼
10 min
125 bp
2.0 ng
∼
9.5 min
100 bp
2.0 ng
∼
9 min
75 bp
2.5 ng
∼
8.5 min
50 bp
2.5 ng
∼
8 min
Appendix E
E
‑
Gel
™
SizeSelect
™
II Agarose Gels
Guidelines for estimating run time
E
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
65
