4.
Remove the top of the gel cassette after all four sides
of the cassette are unsealed.
5.
Proceed to downstream application.
If you plan to transfer DNA from the gel by blotting,
only the main running gel is required. Remove the
upper and lower ion exchange matrix layers and the
well areas with the Gel Knife.
If you plan to purify DNA from the gel, excise the gel
fragment. Transfer the gel slice to a microcentrifuge
tube.
Cleaning and storage
Clean the Gel Knife with mild detergent and water after use, and store at room temperature.
E
‑
Gel
™
agarose gel disposal guidelines
• Discard E
‑
Gel
™
EX Agarose Gels and E
‑
Gel
™
SizeSelect
™
Agarose Gels as hazardous waste.
• SYBR
™
Safe stain is not classified as hazardous waste under US Federal regulations, but contact
your safety office for appropriate disposal methods (see “SYBR
).
Appendix C
E
‑
Gel
™
agarose gels
E
‑
Gel
™
agarose gel disposal guidelines
C
54
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
