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Part # 15050091 Rev. C
Perform a Fluidics Check
The Check button on the Welcome screen performs a fluidics check. Use this option during
instrument installation and when troubleshooting fluidics issues.
1
Load a used flow cell onto the instrument.
2
Load 8 SBS bottles with PW1 or laboratory-grade water, and load the bottles onto the
corresponding reagent rack. Load the rack onto the instrument.
3
Select
Check
on the Welcome screen.
4
Select solution 5 (PB2) from the drop-down list. If you are performing a fluidics check
with a used flow cell, select solution 2, which is water.
5
Enter the following default values:
•
Volume:
250
•
Aspirate Rate:
250
•
Dispense Rate:
2000
6
Select
Pump
. To pause the fluidics check, select
Pause
.
7
Visually inspect the flow cell for bubbles passing through the lanes and leaks near the
manifolds.
If excessive bubbles are present, check the manifold gaskets for obstructions, reduce the
aspirate rate to 100, and pump another 250 µl of water to the flow cell.