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HiSeq X System User Guide
25
Load and Prime Reagents
After entering run parameters, load SBS, indexing, and paired-end reagents for the run, and
then prime reagents through the fluidics system. The software guides you through these
steps in a series of screens on the Pre-Run Setup tab.
Illumina-Supplied Consumables
}
8 funnel caps
User-Supplied Consumables
}
250 ml bottle (Corning, catalog # 430776)
}
15 ml conical tubes (Corning, catalog # 430052)
}
Laboratory-grade water
NOTE
To prepare for the post-run rinse at the end of a sequencing run, load 25 ml PW1 or
laboratory-grade water in position 2.
The post-run rinse does
not
replace the post-run instrument wash.
Load SBS Reagents
1
Invert each bottle several times to make sure that the reagents are mixed thoroughly.
2
Remove the cap from each reagent bottle and replace it with a funnel cap.
CAUTION
After handling the bottle of PCM, discard your gloves and replace them with a new pair.
3
Record the weight of each reagent on the lab tracking form.
NOTE
Weighing reagents before and after a sequencing run confirms proper reagent delivery.
4
Open the reagent compartment door.
5
Raise the sippers for the sequencing reagent rack using the following motion:
a
Pull the handle towards you and then raise the handle.
b
Release the sipper handle into the slot on the top end of the groove. Make sure that
the sipper handle rests securely in the slot.
6
Slide the reagent rack out of the reagent compartment.