Ibi QS-710 Operator'S Manual Download | Manualshive

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IBI

SCIENTIFIC

QS-710 -

Q

UICK

S

CREEN

H

ORIZONTAL

G

EL

E

LECTROPHORESIS

U

NIT

G

EL

S

IZE

:

7

CM X

10

CM

IBI Catalog Number:

IB51000

O

PERATOR

’

S

M

ANUAL

SCIENTIFIC

1
2
3
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Summary of Contents for QS-710

Page 1: ...IBI SCIENTIFIC QS 710 QUICK SCREEN HORIZONTAL GEL ELECTROPHORESIS UNIT GEL SIZE 7CM X 10CM IBI Catalog Number IB51000 OPERATOR S MANUAL SCIENTIFIC ...

Page 2: ...OADING THE SAMPLES INTO THE GEL 7 ELECTRICAL CONNECTIONS TO THE SAFETY LID 8 SAMPLE ELECTROPHORESIS 8 DETECTION AND DOCUMENTATION OF SEPARATED FRAGMENTS 9 CHOICE OF BUFFER 9 VOLTAGE 10 STAINING SOLUTION 10 E MAINTENANCE OF UNIT 11 F REPLACEMENT PARTS ACCESSORIES 11 QS 710 ACCESSORY ITEMS AND REPLACEMENT PARTS 11 QS 710 COMBS 12 G RELATED IBI PRODUCTS 12 H RELATED IBI CERTIFIED REAGENTS 12 14 I REF...

Page 3: ...ted Lid UV Transmittant Casting Tray Casting Fixture One Set of 1 5mm x 8 Tooth Combs 2 ea One Set of Power Cords Red and Black Bubble Level Operation Manual NOTE Carefully inspect all items in the package to insure no items are broken or missing If there are items broken please inspect the package carefully for signs of shipping damage If there is ANY sign of shipping damage please contact the ca...

Page 4: ...e 0 3 means 0 3gm of agarose per 100ml of gel volume and place it into a 250ml flask 3 Make up 500ml of either 1X TAE or 1X TBE electrophoresis buffer See below Electrophoresis Buffers The two most commonly used buffers for horizontal electrophoresis of double stranded DNA in agarose gels are Tris Acetate EDTA TAE IB70160 and Tris Borate EDTA TBE IB70150 While the resolving powers of these buffers...

Page 5: ...Heat the agarose slurry in a microwave oven for 90 seconds Swirl the flask to make sure any grains sticking to the walls enter into the solution undissolved agarose appears as small lenses floating in the solution Heat the solution for an additional 30 60 seconds Re examine the solution and repeat the heating process until the agarose completely dissolves 8 Add deionized water to replace any volum...

Page 6: ...hyde should be kept covered when possible CASTING THE GEL 1 Place the gel casting fixture on a lab bench Check to see that it is level by placing the supplied level ing bubble in the center of the platform The bub ble should appear within the center circle CAUTION Cast agarose gels containing formaldehyde in a hood 2 Insert the gel casting tray into the casting fix ture by pressing one end of the ...

Page 7: ...ressing the casting tray against the foam pad and lifting at the thin pad end Place the tray and gel into the main unit assembly such that the samples wells are on the same end as the negative black electrode see Photo 3 2 Fill the unit with the remaining 1X elec trophoresis buffer containing ethidium bro mide made previously or 1X MAE buffer for RNA gels covering the gel to a depth of 1 5mm Appro...

Page 8: ...or bump the gel box once the lid is place The electrical connection is made by gravity once the lid is in position While this design helps to minimize sam ple disturbance during lid placement it also may result in a disruption of power to the unit if the lid or unit are disturbed during the run SAMPLE ELECTROPHORESIS 1 The QS 710 is designed for quick screen electrophoresis The maximum suggested a...

Page 9: ...le banding patterns may be documented by autoradiography CHOICE OF BUFFER NOTE Tris borate buffer allows faster sample migration than tris acetate buffers with no apparent loss of resolution A tris borate buffer is usually the choice for quick screening Phosphate buffers are used with glyoxal or formaldehyde gels The two most commonly used buffers for horizontal electrophoresis of double stranded ...

Page 10: ...yellow buffer may be used however dark yellow solu tions should be discarded Sample Loading Buffer DNA Sample Loading Buffer RNA 10X Stock Solution 5X Stock Solution 50 Glycerol 1 mM EDTA pH 8 0 100mM Na3EDTA 0 25 Bromophenol Blue 1 SDS 0 25 Xylene Cyanol 0 1 Bromophenol Blue 50 Glycerol pH 8 0 VOLTAGE The QS 710 is designed for rapid electrophoresis with moderate resolution Suggested voltage is 1...

Page 11: ...an electrode breaks contact Technical Support and Information Services at 800 253 4942 for an electrode replacement ELIMINATION OF RNASE CONTAMINATION Should treatment of the unit to eliminate RNase contamination be desired clean the unit with a mild detergent as described above followed by soaking for 10 minutes in a solution of 3 hydrogen per oxide and then 1 hour in 0 1 DEPC diethyl pyrocarbona...

Page 12: ...0 IBI HR 2525 25 X 25cm Horizontal Electrophoresis Unit Comes complete with buffer tank vented lid casting fixture and UVT tray four 2 0mm by 50 tooth combs power cords buffer port set leveling bubble and manual IB62000 IBI VCV Vertical Electrophoresis System 18 X 22cm Vertical Electrophoresis Unit Comes complete with main assembly safety cover three glass plates inner outer and frosted one 1 5mm ...

Page 13: ...IB70056 Agarose Low Melting Point 100gm IB70057 Agarose Low Melting Point 250gm IB70058 Agarose Low Melting Point 500gm IB70059 Agarose Low Melting Point 1Kg IB70052 3 1 Super Sieve Agarose 50gm IB70053 3 1 Super Sieve Agarose 250gm IB70054 Ultra Sieve Agarose 25gm IB70055 Ultra Sieve Agarose 250gm IB70060 Agarose PFGE 25gm IB70061 Agarose PFGE 50gm IB70062 Agarose PFGE 100gm IB70063 Agarose PFGE ...

Page 14: ...AGE 30 sol 29 1 Acrylamide Bisacrylamide 1L IB70004 InstaPAGE 30 sol 37 5 1 Acrylamide Bisacrylamide 500ml IB70005 InstaPAGE 30 sol 37 5 1 Acrylamide Bisacrylamide 1L IB70006 InstaPAGE 40 sol 29 1 Acrylamide Bisacrylamide 500ml IB70007 InstaPAGE 40 sol 29 1 Acrylamide Bisacrylamide 1L IB70008 InstaPAGE 40 sol 37 5 1 Acrylamide Bisacrylamide 500ml IB70009 InstaPAGE 40 sol 37 5 1 Acrylamide Bisacryl...

Page 15: ...ess New York 3 Selden R F 1988 Analysis of RNA by Northern Hybridization in Current Protocols in Molecular Biology F M Ausubel et al editors volume 1 p 4 9 1 Green Publishing Associates and Wiley Interscience J LIMITED WARRANTY Our limited warranty for all electrophoresis gel boxes is four 4 years to the original buyer only non transferable Warranty does not apply to electrodes or platinum wires O...

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