Hologic ThinPrep 5000, Operator'S Manual, Page: 128 / 346

ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
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YNECOLOGIC
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AMPLE
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REPARATION
SAMPLE PREPARATION TROUBLESHOOTING
Because there is biological variability among samples and variability in collection methods, standard
processing may not always yield a satisfactory and uniformly distributed preparation on the first
slide. This section contains instructions for further sample processing to obtain better quality
subsequent slides in these cases.
After staining, you may observe the following irregularities:
• Non-uniform distribution of the cells in the cell spot that was not accompanied by a “Sample
Is Dilute” message.
• Uneven distribution in the form of a ring or “halo” of cellular material and/or white blood
cells
• A sparse cell spot lacking in a cellular component and containing blood, protein, and debris.
This type of slide may be accompanied by a “Sample Is Dilute” message.
Note:
Satisfactory slide appearance is a matter of judgment and experience. Hologic recommends
that you check the quality of the slide after staining. If you determine that the slide is
unsatisfactory, use the procedures in this section to make additional slides.
Note:
Sample preparation troubleshooting as described here has not been evaluated for samples for
the Vysis
®
UroVysion assay.
SECTION
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