Hologic ThinPrep 5000, Operator'S Manual, Page: 123 / 346

N
ON
-G
YNECOLOGIC
S
AMPLE
P
REPARATION
5.16
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5
Procedure for the Use of DiThioThreitol (DTT) with Mucoid Non-Gyn
Samples
DTT has been shown to be a reagent that is effective in reducing the amount of mucus in respiratory
samples.
1
,
2
DTT Stock Solution
• Prepare a stock solution by adding 2.5 g DTT
3
to 30 mL of CytoLyt
™
Solution.
• This solution is suitable for use for 1 week when stored at room temperature (15°C–30°C).
Sample Preparation
• This procedure is designed for mucoid non-gyn sample processing. Follow the steps for
processing mucoid specimens on the previous page.
• After sample collection (Step 1), but prior to vortexing (Step 2), add 1 mL of the stock DTT
solution to the sample.
• Proceed with the remaining sample processing steps as listed.
7. Allow to stand in PreservCyt Solution for 15 minutes (page 5.11)
8. Run on ThinPrep
™
5000 processor with AutoLoader using Sequence
Non-Gyn
. Fix, stain, and evaluate.
1. Tockman, MS et al., ‘Safe Separation of Sputum Cells from Mucoid Glycoprotein’ Acta Cytologica 39, 1128
(1995).
2. Tang, C-S, Tang CMC and Kung, TM, ‘Dithiothreitol Homogenization of Prefixed Sputum for Lung Cancer
Detection’, Diagn. Cytopathol. 10, 76 (1994).
3. Available from Amresco, contact a sales representative at 800-448-4442 or
www.amresco-inc.com.