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Heraeus biofuge pico, Instructions For Use Manual, Page: 1 / 65

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Biofuge

 

Instructions for Use 

 

 

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Summary of Contents for biofuge pico

Page 1: ...Biofuge Instructions for Use...

Page 2: ...ot provide protection against improper handling and thus a gainst damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessories...

Page 3: ...rotor turns speed time quick run set keys open lid start stop back panel socket for mains connection mains switch...

Page 4: ...short term acceleration as long as key is pressed with indication of run time pas sed Set keys stepwise increase decrease of preset values accelerated change when pres sed permanently Short pressing o...

Page 5: ...Transport and installation 17 Mains connection 17 Opening the lid 18 Emergency lid release 18 Inserting the rotor 20 Permissible rotor temperature 21 Lifetime of the rotor 21 Removing the rotor 22 Loa...

Page 6: ...whether autoclaving is permitted 35 The Service of KENDRO 36 Warranty conditions 36 Troubleshooting 37 Problems you can handle yourself 37 In case you must call the Service 42 Technical data 44 Compon...

Page 7: ...pt close at hand at all times Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively The maximum sample den sity is 1 2...

Page 8: ...properly mounted Never manually open the lid if the rotor still turns Use only original parts for the centrifuge The only exception are common glass or plastic centrifuge tubes if these are approved...

Page 9: ...2 2 No 1010 1 92 CAN CSA C22 2 No 1010 2 020 94 Safety instructions in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in i...

Page 10: ...For your safety 6 for your notes...

Page 11: ...d on and the rotor has come to a halt You can start the centrifuge only if the lid is properly locked Warning if lid is manually opened during a run sa fety circuit actuated If the lid is manually ope...

Page 12: ...t speed and run time are displayed before the run During operation the control panel shows the actual values upon briefly pressing any one of the set keys or the preset values for speed and run time a...

Page 13: ...vered complete with a special cap nut for fixing the rotor 10 mm tubular socket wrench for fastening the cap nut fixed angle rotor 24 x 1 5 2 ml 7500 3328 cable for mains connection this Manual cap nu...

Page 14: ...The Biofuge pico 10 for your notes...

Page 15: ...of adapters con taining 24 reduction sleeves each With these adapters you can centrifuge all commercially available microliter tubes with a volume between 0 2 and 0 6 ml as well as 0 2 ml PCR reaction...

Page 16: ...13 000 minimum speed nmin min 1 2 000 2 000 maximum RCF value at nmax 16 060 12 846 11 524 maximum radius cm 8 5 6 8 6 1 minimum radius cm 5 9 6 1 4 7 angle 40 45 acceleration deceleration time s 15...

Page 17: ...0 3328 Adapter Dimensions x H Capacity Number per Set Color Order No reduction sleeve PCR 6 2 x 20 mm 0 2 ml 24 gray 7600 3750 reduction sleeve 8 x 43 5 mm 0 5 0 6 ml 24 turquoise 7600 3758 reduction...

Page 18: ...Accessories 14 for your notes...

Page 19: ...must be kept The centrifuge must be protected from heat and di rect sunshine The location should be well ventilated Mains connection Make sure that voltage and frequency correspond to the specificati...

Page 20: ...Before use 16 for your notes...

Page 21: ...ument Turn off the mains switch on the back panel push down the 0 mark then connect the instrument to the mains sup ply Turn on the mains switch at the back of the centrifuge For a couple of seconds t...

Page 22: ...pped with a mechanical lid unlocking system However you may use this system only in case of emergency Rotor can spin at high speed Touching it may cause severe inju ries Always wait for several minute...

Page 23: ...ks audibly Remove the auxiliary tool and open the lid 4 In case the rotor still turns close lid immediately and wait until it has come to a complete stop Never brake the rotor using your hands or tool...

Page 24: ...to drive and rotor You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and 30 C Proceed as follows 1 Open the lid and make sure that the rotor cham...

Page 25: ...ng of the rotor and retighten the cap nut as nee ded Permissible rotor temperature The rotors are only to be used within the temperature range from 4oC to 40oC Pre cooling in the freezer is not permit...

Page 26: ...the cap nut 3 Grab the rotor in the middle and pull gently upwards off the drive shaft Be careful not to jam it Loading the rotor Maximum loading Overloading may cause the rotor to explode Exploding p...

Page 27: ...e no longer perturbed by vibration It is therefore important to bal ance the centrifuge tubes as well as possible To minimize unbalance you should fill the tubes as evenly as possible You can achieve...

Page 28: ...groove on the screw on top Attention Please check that your sample containers are suitable for the centrifugal application desired 16060 x g temperature in uncooled devices approx 10 K above room temp...

Page 29: ...the amount of test liquid 0 5 so dium fluorescein in water specified in the column leakage test into the lower part of the rotor within a virtual circle comprising the vessel bores not the bores them...

Page 30: ...igure gives examples for proper loading Improper loading can in the worst case lead to damage to rotor and centrifuge Unbalance not only causes a noisy run but rapidly damages the motor suspension Whe...

Page 31: ...sary by repeat edly pressing one of the keys The digit after the decimal point flashes for a number of seconds then changes to permanent display The speed is now stored Selecting the run time You can...

Page 32: ...nning During the run you cannot open the lid Changing the settings during the run You can change the settings during a run The respec tive altered setting flashes for a couple of seconds Once the disp...

Page 33: ...ication During acceleration the time is counted forward in sec onds After 60 seconds the display changes to the min ute mode RCF value The relative centrifugal force RCF is usually given in multiples...

Page 34: ...apters used You may take this into account when calculating the RCF value for your application The figure on the last page of this manual gives a graphic representation of the relation between speed a...

Page 35: ...o make sure by consult ing the manufacturer that the pro cedure foreseen does not cause any damages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor...

Page 36: ...ng a run you have to disinfect the centrifuge im mediately Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon con tact Take appropriate...

Page 37: ...ent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the centr...

Page 38: ...lution and the rotor should not be warmer than ca 25 C 2 Do not let the bleaching solution act longer than ab solutely necessary 3 After disinfection rinse the rotor thoroughly with dis tilled water a...

Page 39: ...subjected to a maximum of 10 autoclavings The rotor must be cleaned and rinsed with distilled wa ter before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic r...

Page 40: ...ontracts cov ering these benefits Inspection costs are charged as flat rate contracts Necessary repairs are carried out free of cost during the warranty period and against payment after expira tion of...

Page 41: ...emain dark The motor stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Is the mains switch turned on 2 Check the mains connection 3 If the mains connectio...

Page 42: ...e 18 B Heat monitoring relays in the lid unlocking magnets ha ve been actuated Press the key again after waiting for about 1 min Centrifuge is exceptionally noisy 1 Stop the centrifuge by pressing the...

Page 43: ...oved If you cannot solve the problem call nearest Service E 8 Overvoltage or over current at the U F converter Mains voltage outside tolerance Brake resistance defective Switch the instrument off and...

Page 44: ...e safety circuit of the motor has been actuated 1 Pull mains plug 2 Control ventilation slots underneath the instrument and clean if necessary 3 After 20 min you can start the instrument again 4 If th...

Page 45: ...Troubleshooting 41...

Page 46: ...software version You can determine the soft ware version as follows 1 Switch the instrument off 2 Switch the instrument on The display reads 888 88 for a couple of seconds Subsequently the display ma...

Page 47: ...Troubleshooting 43 for your notes...

Page 48: ...tion Easycontrol system Rotor chamber Dimensions diameter x height 188 mm x 63 mm Rotor chamber Up to 48 ml of spilled liquids are retained in the rotor chamber and cannot enter the instrument Lid loc...

Page 49: ...Permissible temperature of the environment 2 C to 40 C Maximum speed nmax 13 000 min 1 Maximum RCF value at nmax 16 060 Minimum speed nmin 2 000 min 1 Maximum kinetic energy 1 65 kNm Permissible temp...

Page 50: ...Technical data 46...

Page 51: ...ght LED display in the speed pa nel End of run The speed display reads end Cycle counter Number of cycles is displayed upon pressing the two keys below the speed display Digital parameter display spee...

Page 52: ...page 37 Testing standards 230V instruments Manufactured and tested in accordance with 120 100V instruments Manufactured and tested in accordance with EN 61 010 1 EN 61 010 2 020 EN 61326 EN 61000 3 2...

Page 53: ...Frequency Nominal current Power consumption Fuses inside instrument 7500 3235 230 V 50 60 Hz 1 1 A 150 W 2 x 2 A slow blow 5 x 20 mm 7500 3236 110 120 V 60 Hz 2 A 150 W 2 x 4 A slow blow 6 3 x 32 mm...

Page 54: ...Technical data 50 for your notes...

Page 55: ...centrifuging cycles display 46 changing settings during run 28 cleaning 31 conditions of warranty 36 contamination necessary measures 32 continuous operation 28 control panel readings 8 corrosive subs...

Page 56: ...first steps 15 fixed angle rotor 11 fluorescein test solution for aerosol tightness 25 formula maximum permissible load 22 frequency 15 fuses 47 H hazardous substances 3 hazards symbols used for 5 hin...

Page 57: ...load formula for 22 maximum sample density 3 mechanical lid release 18 O opening the lid 18 operation continuous 28 preselected run time 27 short time 29 organic solvents not allowed for cleaning 32 o...

Page 58: ...ion 28 fine tuning 27 range 27 setting 27 RZB Wert 12 S safety instructions 3 4 safety measures 3 safety standards 5 safety systems built in 7 safety zone 3 30 cm around centrifuge 15 sample density m...

Page 59: ...the environment permissible 44 temperature range for rotor permissible 44 toxins protection against 4 transport precautions for 17 tube breakage with infectious material 32 tubes types 11 volume rang...

Page 60: ...Index 56...

Page 61: ...Autoclaving protocol 57 Autoclaving protocol 1 Date Remark Operator Signature 2 3 4 5 6 7 8 9 10...

Page 62: ...58 Speed RCF diagrams for PCR Rotor 7500 3327 100 1000 10000 100000 10 100 1000 10000 100000 RCF x g speed rpm 13000 12846 rmin 4 7 cm rmax 6 8 cm...

Page 63: ...59 Speed RCF diagrams for 24 x 2ml Rotor 7500 3328 100 1000 10000 100000 10 100 1000 10000 100000 RCF x g speed rpm 13000 16060 rmin 5 9 cm rmax 8 5 cm...

Page 64: ...60...

Page 65: ...l 64 0 9 525 03 33 Fax 64 0 9 525 03 37 info kendro co nz Poland Kendro Sp lka z o o Warsaw Tel 48 0 22 663 43 23 Fax 48 0 22 663 43 25 kendro warszawa kendro com pl Portugal Heraeus S A Massam Tel 35...

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