Harvard Bioscience BTX Oocyte Petri Electrode 45-2059, User Manual

The Harvard Bioscience BTX Oocyte Petri Electrode 45-2059 is a high-quality oocyte petri electrode designed for precise and efficient cell manipulation. To assist users in maximizing their experimental success, a comprehensive User Manual is available for free download from our website. Gain valuable insights and operating instructions to excel in your research with this exceptional product.

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Oocyte

Petri Electrode

45-2059

45-2060

15 mm Oocyte Petri Electrode, Platinum,

1 mm gap (electrode only)

15 mm Oocyte Petri Electrode, Platinum,

1 mm gap, Kit (45-2059, 45-0503, 45-0204)

User’s Manual

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Summary of Contents for BTX Oocyte Petri Electrode 45-2059

Page 1: ...Oocyte Petri Electrode 45 2059 45 2060 15 mm Oocyte Petri Electrode Platinum 1 mm gap electrode only 15 mm Oocyte Petri Electrode Platinum 1 mm gap Kit 45 2059 45 0503 45 0204 User s Manual ...

Page 2: ...n If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Note BTX electrodes are not recommended for use with power supplies or cables from other manufacturers Such use is completely at the ...

Page 3: ... medium might lead to flashover between the electrodes ARC and or explosive evaporation of the medium Reduce voltage or pulse length to avoid repeating this condition DO NOT OPERATE WITH SUSPECTED FAILURES If you suspect there is damage to the product have it inspected by qualified BTX service personnel DO NOT CONTACT ELECTRODES To avoid fire or shock hazard observe all ratings and markings on the...

Page 4: ...es can be visualized during electroporation This electrode is particularly useful for the generation of transgenic animals including use with CRISPR Cas 9 constructs to both knock in and knock out targeted genes The Oocyte Petri Electrode may be cleaned with a mild detergent and sterilized with 75 ethanol This electrode requires the use of Mini Micro Grabber Cables 45 0503 and Tweezertrode Adapter...

Page 5: ...s on the slide Suggested fill volumes are 7 µl for 70 embryos and 10 µl for 100 120 embryos 5 Deliver the electroporation pulse s to the sample 6 Recover sample with wide bore pipette tip During a session of multiple electroporations using the same transfectant excess buffer may be removed with a sterile eye spear in between rounds of electroporation 7 Disconnect from Mini Micro Grabber cables and...

Page 6: ...20 to 80 Physical Characteristics Item No Electrode Length Electrode Gap Electrode Material 45 2059 15 mm 1 mm Medical Grade Polished Platinum Compatibility Generators BTX Gemini X2 ECM 830 ECM 2001 Please contact BTX Technical Service at any of the numbers listed below in the event of any failure BTX A Division of Harvard Bioscience 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 8...

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