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HI96801

Refractometer for Sucrose 

Measurements

INSTRUCTION MANUAL

Summary of Contents for HI 96801

Page 1: ...HI96801 Refractometer for Sucrose Measurements INSTRUCTION MANUAL ...

Page 2: ... this instrument as well as a precise idea of its versatility If you need additional technical information do not hesitate to e mail us at tech hannainst com or view our worldwide contact list at www hannainst com Dear Customer All rights are reserved Reproduction in whole or in part is prohibited without the written consent of the copyright owner Hanna Instruments Inc Woonsocket Rhode Island 0289...

Page 3: ...ions Principle of Operation Functional Description Display Elements Measurement Guidelines Calibration Procedure Measurement Procedure Making a Standard Brix Solution To Change Temperature Unit Battery Replacement Error Messages Accessories 4 5 7 9 11 16 6 8 12 10 17 15 14 18 ...

Page 4: ...s occurred contact your local Hanna Instruments Office Each HI96801 instrument is supplied with 9 V battery Instruction manual Plastic pipette Quality Certificate Note Save all packing material until you are sure that the instrument functions correctly A defective instrument must be returned in its original packing PRELIMINARY EXAMINATION ...

Page 5: ...d converts it to Brix concentration units TheHI96801digitalrefractometereliminatestheuncertainityassociatedwith mechanicalrefractometersandiseasilyportableformeasurementsinthefield Themeasurementtechniqueandtemperaturecompensationemploymethodo logy recommended in the ICUMSA Methods Book International Commission for Uniform Methods of Sugar Analysis Temperature in Cor F isdisplayedsimultaneouslywit...

Page 6: ...mpensation Automatic between 10 0 and 40 0 C 50 0 104 0 F Measurement Time Approximately 1 5 seconds Minimum Sample Volume 100 µL cover prism totally Light Source Yellow LED Case Material ABS Enclosure Rating IP65 Battery Type Life 9V 5000 readings Auto Off After 3 minutes of non use Dimensions 192 x 102 x 69 mm 7 6 x 4 1x 2 7 Mass 350 g 12 3 oz ...

Page 7: ...Refractive Index is defined as the ratio of the speed of light in empty space to the speed of light in the substance A result of this propertyisthatlightwill bend orchangedirection whenittravelsthrough a substance of different refractive index This is called refraction When passing from a material with a higher to lower refractive index there is a critical angle at which an incoming beam of light ...

Page 8: ...ay LCD 2 Secondary Display 3 Primary Display 4 READ Key User Measurement 5 ZERO Key User Calibration 6 ON OFF 7 Stainless Steel Sample Well and Prism TOP VIEW FUNCTIONAL DESCRIPTION 8 Battery Cover 9 Battery Compartment BOTTOM ...

Page 9: ...ss Tag 4 SETUP Factory Calibration Tag 5 CAL Calibration Tag 6 Measurement Units 7 Automatic Temperature Compensation blinkswhentemperatureexceeds10 0 40 0 C 50 0 104 0 Frange 8 Temperature Units 9 SecondaryDisplay displaystemperaturemeasurements whenblinking temperaturehasexceededoperationrange 0 0 80 0 C 32 0 176 0 F DISPLAY ELEMENTS ...

Page 10: ...nic solvents and extremely hot or cold solutions Particulate matter in a sample may scratch the prism Absorb sample with a soft tissue and rinse sample well with deionized or distilled water between samples Use plastic pipettes to transfer all solutions Do not use metallic tools such as needles spoons or tweezers as these will scratch the prism To reduce the effects of evaporation or absorption of...

Page 11: ...ts or if environmental changes have occurred since the last calibration 2 Using a plastic pipette fill the sample well with distilled or deionized water Make sure the prism is completely covered Note If the ZERO sample is subject to intense light such as sunlight or another strong source cover the sample well with your hand or other shade during the calibration 1 Press the ON OFF key then release ...

Page 12: ...prism surface Fill the well completely Note If the temperature of the sample differs significantly from the temperature of the instrument wait approximately 1 minute to allow thermal equilibration CALIBRATION PROCEDURE 4 Gently absorb the ZERO water standard with a soft tissue Use care not to scratch the prism surface Dry the surface completely The instrument is ready for sample measurement Note I...

Page 13: ...emperature compensation is disabled if the temperature exceeds the 10 0 40 0 C 50 0 104 0 F range MEASUREMENT PROCEDURE 4 Remove sample from the sample well by absorbing with a soft tissue 5 Using a plastic pipette rinse prism and sample well with distilled or deionized water Wipe dry The instrument is ready for the next sample 3 Press the READ key The result is displayed in the selected units ...

Page 14: ...x Brix g Sucrose g Water g Total 25 25 000 75 000 100 000 To make a Brix Solution follow the procedure below Place container such as a glass vial or dropper bottle that has a cover on an analytical balance Tare the balance To make an X BRIX solution weigh out X grams of high purity Sucrose CAS 57 50 1 directly into the container Add distilled or deionized water to the container so the total weight...

Page 15: ...imately 15 seconds The LCD will display the all segment screen followed by a screen with the model number on the primary display and the version number on the secondary display Continue pressing the ON OFF key 2 While continuing to hold the ON OFF key press the ZERO key The temperature unit will change from C to F or vice versa 15seconds TO CHANGE TEMPERATURE UNIT ...

Page 16: ...re the instrument is off Turn instrument upside down and remove the battery cover by turning it counterclockwise Extract the battery from its location Replace with a new 9V battery making sure to observe polarity Insert the back battery cover and fasten it by turning clockwise to engage ...

Page 17: ...display Cal segment ON Temperature exceeds ATC low limit 10 0 C during calibration t HI primary display Cal segment ON Temperature exceeds ATC high limit 40 0 C during calibration Air Prism surface insufficiently covered ELt Too much external light for measurement Cover sample well with hand nLt LED light is not detected Contact your local Hanna Instruments Office Battery segment blinking 5 of bat...

Page 18: ...18 Code Description HI4020 11 50 Brix Standard 10 mL HI740157P Plastic refilling pipette 20 pcs HI740029P 9V Battery 10 pcs ACCESSORIES MAN96801 04 17 ...

Page 19: ...umber and the nature of the problem If the repair is not covered by the warranty you will be notified of the charges incurred If the instrument is to be returned to Hanna Instruments first obtain a Returned Goods Authorization number from the Technical Service department and then send it with shipping costs prepaid When ship ping any instrument make sure it is properly packed for complete protecti...

Page 20: ...ments Inc Highland Industrial Park 584 Park East Drive Woonsocket RI 02895 USA www hannainst com Local Office Hanna Instruments USA 270 George Washington Highway Smithfield RI 02917 Phone 800 426 6287 Fax 401 765 7575 e mail tech hannainst com MAN96801 ...

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