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SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
Instruction Manual
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product. Please read the
instructions carefully before using the chemical test kit. It
will provide you with the necessary information for correct
use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable damage,
notify your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
• 1 HI 180IMD magnetic stirrer;
• HI 3897-0 Titrant Solution, 1 bottle (20 mL);
• Organic Solvent, 6 bottles (40 mL each) with one
magnetic bar each;
• 1 calibrated syringe of 5.0 ml;
• 1 calibrated syringe of 1.0 ml with tip.
Note:
Note:
Note:
Note:
Note: Any damaged or defective item must be returned in
its original packing materials.
Range
0 to 1% acidity
Smallest Increment
0.01 ml = 0.01 %
Analysis Method
Titration
Sample Size
4.6 mL (or 4.0 g)
Number of Tests
6
Case Dimensions
112 x 390 x 318 mm
Shipping Weight
3000 g
HI 3897
Extra Virgin
Extra Virgin
Extra Virgin
Extra Virgin
Extra Virgin
Olive Oil Acidity
Olive Oil Acidity
Olive Oil Acidity
Olive Oil Acidity
Olive Oil Acidity
TTTTTest Kit
est Kit
est Kit
est Kit
est Kit
ISTR3897R2 07/04
The oil sample is firstly dissolved in an organic solvent
reagent (ethanol/ether); then it is titrated with a basic
solution (OH
-
). The final point of the titration is indicated by
the change in color of the solution, which turns from
yellow/green of the oil to pink.
• Remove the cap from 1 bottle of Organic Solvent
reagent. Place the bottle on the magnetic stirrer and
start a gentle stirring.
• Using the 1.0 ml syringe with tip, add the HI 3897-0
Titrant Solution reagent dropwise. Continue adding the
Titratant Solution reagent until the solution in the
bottle changes from colorless to a persistent light pink.
Replace the cap.
Note
Note
Note
Note
Note: stop adding the HI 3897-0 Titrant Solution
reagent to the bottle as soon as the solution is persistently
light pink colored.
• Take 4.6 ml of oil sample by use of the 5.0 ml syringe.
Alternatively, weight about 4.0 g (“w” grams) of oil by
use of an analytical balance.
Note
Note
Note
Note
Note: to dose exactly 4.6 ml of the oil sample:
(a) push the plunger completely into the syringe,
(b) insert the tip into the oil sample,
(c) pull the plunger up until the lower edge of the seal
is exactly on the 5 mL mark,
(d) take out the syringe and clean the outside of the
syringe tip. Be sure that no drops are hanging on
the tip of the syringe, if so eliminate them,
(e) then, keeping the syringe in vertical position above
the oil sample, push the plunger down into the
syringe, till the 4.6 ml sign,
(e) be sure that no drops are hanging on the tip of
the syringe, if so eliminate them,
(g) then, keeping the syringe in vertical position above
the (open) bottle of Organic Solvent, push the
plunger completely down into the syringe. Now the
exact amount of 4.6 mL has been added to the
bottle.
Specifications
Significance and Use
Chemical Reaction
Instructions
Acidity defined as oleic acid, is a parameter that indicates
the oil freshness: a high acidity value shows the oil is
becoming rancid, thus the oil quality is decreasing. According
to CEE 2568/91
CEE 2568/91
CEE 2568/91
CEE 2568/91
CEE 2568/91 regulation, olive oil is called extra virgin
when its acidity is below 1%: see table 1.
Acidity expressed in oleic acid is used to discriminate extra
virgin olive oil from other olive oils. It indicates an extraction
process made soon after the olive harvesting and with
natural, non chemical methods.
CAUTION!
Carefully open the bottle of the Organic Solvent
reagent because it may be under pressure!
READ ALL INSTRUCTIONS BEFORE USING THE TEST KIT
Table 1
• Remove the cap and add the oil sample to the Organic
Solvent bottle. Replace the cap.
• Place the sample on the magnetic stirrer and stir gently
until all the oil sample is dissolved in the Organic
Solvent reagent.
• Using the 1.0 ml graduated syringe with tip, take
1.0 ml of HI 3897-0 Titrant Solution reagent.
Note
Note
Note
Note
Note: to dose exactly 1.0 ml of the Titrant Solution
reagent:
(a) push the plunger completely into the syringe,
(b) insert tip into HI 3897-0 Titrant Solution reagent,
(c) pull the plunger out until the lower edge of the
plunger seal is on the 0 mL mark of the syringe,
(d) take out the syringe and clean the outside of the
syringe tip. Be sure that no drops are hanging on
the tip of the syringe, if so eliminate them.