Instruction Manual
Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the chemical
test kit. It will provide you with the necessary information for
correct use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable damage,
notify your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
• HI 3874-0 Nitrate Reagent, packets (100 pcs);
• 1 glass cuvet;
• 1 color comparator cube.
Note:
Any damaged or defective item must be returned in
its original packing materials.
HI 3874
Nitrate
Test Kit
ISTR3874R2 02/07
Range
0 to 50 mg/L (ppm) as NO
3
-
-N
Smallest Increment
10 mg/L (ppm) NO
3
-
-N
Analysis Method
Colorimetric
Sample Size
10 mL
Number of Tests
100
Case Dimensions
230x59x70 mm (9.0x2.3x2.8")
Shipping Weight
156 g (6.0 oz.)
• Fill the glass cuvet with 10
mL of the sample, up to
the mark.
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Specifications
Significance and Use
Chemical Reaction
Instructions
Safety Data Sheets
Accessories
HI 3874-100
replacement kit (100 tests)
HI 3874-99
color comparator cube for nitrate kit
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Safety Data Sheet
before performing this test.
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Add 1 packet of HI 3874-0 Nitrate Reagent.
• Replace the cap
and shake vigorously for exactly
1 minute. A deposit may remain, but it will not affect
measurement. Time and manner of shaking can affect
the results.
• Wait for 4 minutes to allow the color to develop. Remove
the cap and fill the color comparator cube with 5 mL of
the treated sample (to the mark).
• Determine which color matches the solution in the cube
and record the result in mg/L (or ppm) of Nitrate-nitrogen.
• It is better to match the color with a white sheet at about
10 cm behind the comparator.
• To convert the reading to mg/L of Nitrate (NO
3
-
), multiply
the reading by a factor of 4.43.
Nitrate ions are present in trace amounts in surface water
and in higher levels in some groundwater. Nitrate is found
only in small quantities in domestic wastewater but can
reach higher concentration (up to 30 mg/L as nitrogen) in
the outflow of nitrifying biological treatment plants. Excessive
amounts can contribute to methaemoglobinemia: infant death
and adult illness. In order to prevent this, a 10 mg/L limit
(as nitrogen) has been imposed on drinking water.
NOTE:
NOTE:
NOTE:
NOTE:
NOTE: mg/L is equivalent to ppm (parts per million).
Nitrates are reduced to nitrites in the presence of Cadmium.
The nitrites thus produced react with the reagent to yield an
orange compound. The amount of color developed is
proportional to the concentration of nitrate present in the
aqueous sample.
Adaptation of the cadmium reduction method from Standard
Methods for the Examination of Water and Wastewater, 20
th
Edition, 1998.
References
