Instruction Manual
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the
test kit. It will provide you with the necessary information
for correct use of the kit. If you need additional information,
do not hesitate to e-mail us at [email protected].
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
• Buffer Solution pH 10.2±0.2, 1 bottle (100 mL);
• Calmagite Indicator, 1 bottle with dropper (10 mL);
• HI 38080-0 EDTA Solution, 1 bottle (120 mL);
• 1 calibrated vessel (50 mL);
• 1 plastic pipette (3 mL);
• 1 plastic pipette (1 mL);
• 1 syringe (1 mL) with tip.
Note:
Any damaged or defective item must be returned in
its original packing materials.
HI 38080
Calcium and
Magnesium
Test Kit for Soil
ISTR38080 09/00 PRINTED IN ITALY
Calcium and Magnesium are often present in soil as carbon-
ates (e.g.: dolomite), sulfates (in arid regions) and silicates.
They are necessary nutrients for plants since they have an
important role in plant metabolism and growth.
They can be removed from soil by leaching (for instance in
acidic soil of humid regions) or by crop production; a
deficiency of calcium and magnesium in soil will manifest
itself in stunted growth and in yellow and deformed leaf
tips. On the other hand, soil with an excess of Calcium and
Magnesium will lock up other necessary micronutrients,
making them unavailable to plant roots (e.g.: available
phosphorus forms an insoluble salt with calcium at pH
values above 7.5, thus decreasing the efficiency of applied
phosphorus fertilizers).
Note:
meq/100 g is milliequivalent per 100 grams.
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
Range
> 0 meq/100 g
Smallest Increment
1.5 meq/100 g
Analysis Method
Titration
Sample Size
1.0 mL
Number of Tests
100 (average)
Case Dimensions
195x123x53 mm (7.7x4.8x2.1")
Shipping Weight
336 g (11.8 oz.)
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Obtain a Mehlich extract from the soil sample by
performing the
Mehlich extraction
.
•
Remove the cap and fill
the Demineralizer Bottle
with tap water.
• Replace the cap and
shake for at least 2
minutes. The deminer-
alized water is now
ready.
• Using the syringe, transfer
1 mL of soil extract to the
calibrated vessel.
Note:
to measure exactly 1 mL
of sample, take the sy-
ringe and push the
plunger completely down
into the syringe. Insert
tip into sample and pull
the plunger out until the
lower edge of the seal is on the 0 mark of the
syringe. Insert the syringe into the vessel and push
the sample out until the lower edge of the seal is on
the 1.0 mL mark.
• Flip open the top of the Dem-
ineralizer Bottle cap. By gently
squeezing the bottle, add dem-
ineralized water to the plastic
vessel up to the 25 mL mark.
• Using the 1 mL pipette, add 1 mL of Buffer Solution
and swirl to mix.
• Add 2 drops
of Calmagite
I n d i c a t o r
and swirl to
mix. If cal-
cium and
magnesium
are present, the solution will turn wine red.
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
The Hanna Test Kit extracts Calcium and Magnesium from
soil in acidic medium and then determines them via a
titrimetric method: the indicator chelates with the Calcium
and Magnesium ions to form a red colored complex. As EDTA
is added, calcium and magnesium complex with it: the
reaction endpoint is indicated by a change in color of the
indicator from red to blue.
• Using the 3 mL plastic pipette, add drops of HI 38080-0
EDTA Solution, swirling after each drop, while keeping
an accurate count of the number of drops being added
to the solution.
• As the color changes from pink to purple, swirl for 15
seconds after each additional drop, until the solution
turns pure blue. Record the number of drops needed to
obtain the final color change (from wine red to pure
blue).
Note:
High amounts of copper in your sample will alter the
final endpoint color. The solution will change from wine
red to purple, without turning pure blue. In this case,
add drops of titrant until no visible change in color is
obtained.
• Calculate the meq/100 g of Calcium and Magnesium in
your sample as follows:
meq/100 g [Ca +Mg]
= drops of Titrant x 1.5
• Rinse all labware with demineralized water after each
analysis and shake dry.
The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
REFERENCES
REFERENCES
REFERENCES
REFERENCES
REFERENCES
Adaptation of the
Standard Methods for the Examination
of water and wastewater
, 18
th
Ed. 1992, APA AWWA
WEF.
P. Sequi,
Chimica del suolo
, Patron Editore, Ed. 1991
1 mL
1 mL
25 mL
