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Chapter 6, General Operation: Sequestration
P/N 120745-IE, Manual revision: AA
Haemonetics
®
Cell Saver
®
Elite
®
User Manual
Performing a Sequestration Procedure
Procedure
Overview
During the Sequestration procedure the device separates the blood into
platelet poor plasma (PPP), platelet rich plasma (PRP), and red blood cells
(RBCs).
Prior to beginning Sequestration, blood is collected into blood bags containing
anticoagulant (AC) solution. During Sequestration, the device processes blood
in a series of cycles. Each cycle consists of a Fill and Empty phase. During the
Fill phase, the bowl fills with whole blood and then collects PPP and PRP in the
collection bags. The device displays prompts throughout the procedure that
instruct you to open and close clamps at the appropriate times.
The Sequestration procedure is usually performed just after the induction of
anesthesia but prior to the surgical procedure and is only available when the
device is first powered on and prior to starting the first Fill phase in the Cell
Salvage procedure. Suction may be turned on while performing Sequestration
if you want to start collecting fluid from the surgical field.
The system is first set up with a standard processing set. A Sequestration set
is then attached to the processing set as described in this chapter.
Prior to running the Sequestration protocol with the Cell Saver Elite device, you
should be familiar with the operating instructions and all associated
precautions and warnings for the device, in addition to any precautions and
warnings specifically related to Sequestration provided in this chapter.
Note: You may turn on the on-board suction and begin collecting fluid in the
reservoir while Sequestration is being completed.
Processing
from Blood
Bags
Warning: Only one unit of blood should be collected and processed at a time.
Unless directed by a physician, do not begin withdrawal of the second unit of
blood from the patient until the first unit of concentrated autologous RBCs
has been reinfused to the patient.
Prior to the Sequestration procedure, collect whole blood from the patient via
a short intravenous or arterial cannula into blood bag(s) containing
anticoagulant solution.
Initiating a
Procedure
Once the disposable set is properly loaded onto the device, follow the steps
below to draw blood into the bowl from the blood bag:
1. Touch
Start Procedure
to start Sequestration. The device advances to
Standby.
2. Touch
Fill
.
The device displays prompts throughout the procedure that instruct the
user to open and close the white, blue, and yellow clamps on the
