P/N 53063-50, Manual Revision: B
Equipment and Disposable Description
2-19
Filling the
Centrifuge Bowl
Once the disposable has been loaded the CS5+ displays a current setting screen.
The operator may choose to restore default settings and then enter STANDBY
mode. The CS5+ then automatically starts a FILL cycle when the predetermined
level of fluid has been collected into the reservoir (the default level is 800 ml; the
operator can press START a second time to initiate a FILL cycle before this level
is reached). At that point the centrifuge bowl begins spinning, and the red line
valve opens. The pump begins to FILL the bowl by transferring fluid from the
reservoir while keeping track of how much fluid it is pumping. Note that on the
first cycle (only) of a procedure, the saline line will automatically be primed
before the red line valve opens.
Cell Separation
and Concentration
in the Bowl
The spinning of the centrifuge traps the heavier and denser red blood cells and
causes them to move toward the outer walls of the centrifuge; red blood cells
form the outer layer while the supernatant floats inward toward the core of the
bowl. The lighter fraction is forced out the effluent (exit) tubing from the bowl and
into the waste bag.
Red Cell Washing
After the process described above has been completed, the optical RBC sensor
located in the centrifuge well will detect that the RBC content of the bowl is
sufficient (approximately 50% hematocrit) to warrant washing. When utilizing
the 70 ml bowl, the effluent line sensor detects the full bowl.
The optical RBC sensor (or effluent line sensor for the 70 ml bowl) will cause the
red fill line to be closed, and the yellow wash line to be opened, allowing wash
solution to enter the bowl and wash the RBCs. Washing the RBCs further removes
unwanted components such as cell stroma, free hemoglobin, activated clotting
factors, platelets, and anticoagulant.
Warning: Normal saline is the recommended wash solution for use with the
Haemonetics Cell Saver 5+.
At the start and finish of the WASH mode there is a brief “switching” of the valves
that the operator may notice. At the start of the WASH mode, the blue line valve
opens for one pump revolution so that the few milliliters of fluid that may have
entered this line during the FILL mode will be drawn out (and not sent to the
reinfusion bag).
At the end of the WASH mode, just before the system enters the EMPTY or
RETURN mode, the red valve opens for two pump revolutions, and then closes
while the blue valve opens. The two pump revolutions force any wash solution
left in the line into the red FILL line where it will be returned to the bowl and
eventually sent to the waste bag during the next FILL mode. If this wash solution
was left in the line it would be sent to the reinfusion bag where it would dilute
the end product.
