P/N 53063-30, Manual revision: B
6-7
Cell Saving using Manual Operation
The initial fill speed will be 400 ml/min until the bowl type is determined
and the preset parameters will be in effect. The recommended maximum fill
speeds are listed at the end of this chapter in
Using the CONCENTRATE state
To manually use the concentration option during the final fill cycle:
"
Press the backlit Conc key.
The blue pinch valve will open and allow RBCs to be drawn from the reinfu-
sion bag into the bowl and displace supernatant from the bowl into the
waste bag.
Washing the cells
As the bowl is filling during either the FILL or CONCENTRATE state, the
operator should observe when the RBC/supernatant interface reaches a
point just below the neck of the bowl. At this point the operator can initiate
a wash cycle as follows:
"
Press the Wash key to receive the following information:
Figure 6-7, Example of the WASH state screen display (manual)
The volume of wash solution required will depend upon factors such as the
degree of hemodilution and amount of AC solution used. Certain minimum
volumes will be required per cycle depending on the type of procedure. The
operator should continue washing until the effluent line is clear. The recom-
mended maximum wash speed and wash solutions volumes are listed for all
bowl types in the section at the end of this chapter,
.
Note: If during the WASH state the RBC/supernatant interface disappears
and RBCs begin to spill into the waste bag, the pump speed can be reduced
to zero to allow the interface to reappear, then slowly increased to a rate
that just maintains this interface (usually 200 ml/min of wash solution or
greater).
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