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PIPETMAN® M
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USER’S GUIDE
REVERSE Mode
The
REVERSE Mode
is reverse pipetting with a manual pipette. During
aspiration, additional liquid is added. After delivery, the excess volume
remains in the tip and is discarded.
The
REVERSE Mode
is ideal for pipetting viscous liquids; liquids with high vapor
pressure or those that tend to foam. Your PIPETMAN M aspirates a selected volume
and an excess. This excess compensates for the liquid that remains as a film inside the
tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell
culture, buffer preparation and, many others.
Electrophoresis Gels Loading Protocol
Electrophoresis gels loading protocol: first change the pipetting speed of your pipette
(refer to Chapter 7
on page 13). Aspiration can be done by
using the standard speed (by default speed 6). Dispensing should be done very slowly
and carefully, to prevent swirling and spilling of the samples, so select the lowest speed 1.
1.
Press mode button to access system menu, where you can choose between
different pipette modes. Select
REVERSE Mode
by turning the push button.
To confirm your selection click, the push button.
Set the volume
1.
Press the push button, set the volume by turning the push button and press again
to confirm your settings.
2. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use
PIPETMAN DIAMOND Tips for assurance of accurate and precise results; refer to
Chapter 8
on page 13).
Aspirate
Press push button to aspirate the selected volume.
An amount of liquid equal to the amount of purged
air is added. The volume aspirated will be a little bit
more than the set volume.
Dispense
Press push button to dispense the volume, the
additional amount of liquid remains in the tip.
Purge
Press push button, ‘‘
PURGE
’’ appears on the
screen, press again to purge and reset the piston.
Keep your thumb pressing the push button until
the end of the purge: the piston reset takes place
after you release the push button again.
This feature enables you to remove the tip from
the liquid, without aspirating anything.
Eject tip
Press the ejection button.
NOTICE
Always fit a tip before using any pipette.
0
Purge
START button
Optional STOP
Start Purge
V (+ extra vol)*
Click to Purge
Figure 5
REVERSE Mode
NOTICE
If you purge without pulling out the tip air bubbles could get into your gel!
