07MAN1054.A2
Effective Date: 27-May-11
ClonePix
TM
FL
Robot Manual
Page 1: ...07MAN1054 A2 Effective Date 27 May 11 ClonePixTM FL Robot Manual ...
Page 2: ...tion 12 Layout of ClonePix FL and Ancillary Equipment 12 Source and Destination Plate Stackers 13 Connecting the Wash Bottles 14 Connecting the Ethanol Bottles 14 Camera Alignment 15 ClonePix FL Panel 15 Health and Safety 16 Important Note 16 Transport and Storage 16 Lifting Points 16 External Covers 16 Safety Features 17 Electrical Safety 18 Drive Safety 18 Hot Air Halogen Dryer 18 Noise Levels 1...
Page 3: ...Automated Picking Pin Cleaning 25 Sterility Validation Tests 25 Changing the Picking Head 26 Remove Head 26 Replace Head 26 Changing or Replacing Picking Pins 26 Replacement Parts and Optional Extras 27 Replacement Parts 27 Optional Extras 27 Reagents and Supplies 28 CloneDetect Detection Reagents 28 CloneMatrix 29 CloneMedia and XP Media 29 PetriWell Cell Culture Plates 30 Other 31 Troubleshootin...
Page 4: ...ure 1 ClonePix FL electrical connections 8 Figure 2 ClonePix FL PC connections 9 Figure 3 ClonePix FL layout and dimensions 12 Figure 4 Stacker orientation 13 Figure 5 Stackers and cassettes 14 Figure 6 Connecting the wash bottles 14 Figure 7 ClonePix FL Panel 15 ...
Page 5: ... of five filter sets Colonies selected on the basis of size shape and proximity to neighbors Colonies ranked based on fluorescent intensity of the colony or the secreted product Automated selective picking using high precision picking pins with proprietary cleaning routine between each pick Intuitive Windows based software allows you to easily set up multiple picking processes and analyze the resu...
Page 6: ...height Weight Robot 350 kg maximum Compressor 60 kg Compressed Air Supply Minimum Pressure 6 bar Minimum volume 80L min Operating Environment Indoor use only Temperature 10 C to 40 C Humidity 20 to 80 non condensing Altitude Up to 2000M Mains supply 10 Rated Voltage Transient overvoltage Installation Category Overvoltage category II Rated pollution Pollution degree 2 ...
Page 7: ...ut PC IEC Output Monitor Fuses Input F3 T10A Robot Halogen Heater Output F2 T5A Computer and Monitor Compressor 230V AC 50Hz 3 4A USA Japan Voltage 100 125V AC 45 66 Hz single phase Power Robot 1250W Halogen Heater 300W Connections IEC Input Robot Mains Halogen Heater IEC Output PC IEC Output Monitor Fuses Input F3 T10A Robot Halogen Heater Output F2 T5A Computer and Monitor Compressor 120V AC 60H...
Page 8: ...tions 2007 The year of manufacture is given on the serial number label which is located on the right side of the unit European Economic Area EEA This mark on the product indicates compliance with the following EEC Directives 98 37 EEC relating to The Supply of Machinery Safety Regulations 1992 89 336 EEC with amendments 92 31 EEC relating to Electromagnetic Compatibility 73 23 EEC the Low Voltage ...
Page 9: ...GENETIX CLONEPIX FL ROBOT MANUAL 9 of 37 PC Connections Figure 2 ClonePix FL PC connections ...
Page 10: ...tandard CloneDetect FITC Fluorescein GFP AF488 470 Blue 3 535 Green 3 X4992 YFP 500 Cyan 2 550 Yellow 2 X4993 CloneDetect 549 R phycoerythrin Rhodamine AF546 530 Green 1 590 Red 1 X4994 CloneDetect 649 Cy5 FMAT Blue AF647 622 Red 6 700 Far Red 6 Notes Refer to Reagents Supplies section for details on how to order CloneDetect reagents White light imaging uses excitation filter position 7 and emissi...
Page 11: ... This creates a horizontal laminar flow of sterile air across the picking area of the bed of the machine whilst the front door is closed The main features are Class 100 type enclosure Offers product protection from particulates and microbes HEPA filtration 99 999 at 0 3 microns exclusion Laminar airflow across the bed Air inflow greater than air exhaust maintains positive air pressure Always on wh...
Page 12: ...FL ROBOT MANUAL 12 of 37 Installation Note Installation is to be undertaken only by Genetix approved personnel Layout of ClonePix FL and Ancillary Equipment Front view Top view Figure 3 ClonePix FL layout and dimensions ...
Page 13: ...re then placed into the respective stacker The plates are automatically fed onto the bed individually and the lid is removed as the plate is pushed past the return cassette location The lid is replaced as the plate moves to the return cassette Figure 4 Stacker orientation To insert the feed cassettes push firmly into place until the cassette sits in the stacker The return cassettes have a locking ...
Page 14: ... make sure that they are marked so that the tubes can be re connected correctly The outlets on the machine are in the same order as the bottles on the wash station Figure 6 Connecting the wash bottles Connecting the Ethanol Bottles The tubing carrying ethanol to the Ethanol Wash Bath and back to the ethanol waste bottle will be connected by a Genetix approved engineer on commissioning of ClonePix ...
Page 15: ...lignment are performed before ClonePix FL is dispatched and will be checked by a Genetix approved engineer when the robot is installed If the actuator is subject to a knock or a crash picking accuracy may be affected Therefore it may be necessary to re calibrate the camera This should be carried out by a Genetix approved engineer ClonePix FL Panel Figure 7 ClonePix FL Panel The lights on the panel...
Page 16: ...e of movement Lifting Points The machine should not be moved after installation If relocation is necessary standard lifting gear is adequate but should be undertaken only in the presence of a Genetix approved engineer The robot should be moved into position using appropriate handling equipment such as forklift trucks or dolly trucks The machine should be properly balanced on the forks prior to lif...
Page 17: ...amage It disables the UV light therefore preventing the risk of damage from UV radiation The door is made from acrylic and so prevents UV light from passing through during operation UV Light ClonePix FL is fitted with a UV germicidal lamp with timer This is a 30W linear discharge lamp with a sharply defined output at 253 7nm making the lamp an efficient source of germicidal radiation Emergency STO...
Page 18: ... fitted where appropriate Drive Safety Be aware that the motors use high powered magnets The linear drive units and encoders are delicate so great care should be taken with them The Pre Power Up checklist should be followed before every routine in order to prevent serious damage to the robot or any of its constituent parts Power is removed from the drives when the doors are open Hot Air Halogen Dr...
Page 19: ...oplates or 12 Genetix microplates The Z ball screw drive carries a unique actuator system that accommodates the head This system allows for easy exchange and set up of the head Pre Power Up Checklist Check that the Emergency Stop button see Figure 7 on page 15 is released pulled out ClonePix FL will not start if the button is pushed in Check that no items have been left on the bed of the instrumen...
Page 20: ...n is pressed red light goes off Once the computer has booted up click on the ClonePix FL icon The first time ClonePix FL is used the three axes will sequentially go through their Initialize drives routine This enables the drives to find their respective datum positions ClonePix FL must be allowed to complete this process without interference to ensure that there is no damage to the instrument or i...
Page 21: ...thanol For systems fitted with a replenishing ethanol wash bath check that there are no kinks in the supply waste tubing and then run through with 70 ethanol Check that the bath fills with ethanol and that the waste ethanol flows freely into the waste bottle On first use it may be necessary to run through several times Weekly In any week that ClonePix FL is not used it is important to purge the sy...
Page 22: ...of 70 Ethanol 3 Depress highest piston to allow interior to fill 4 Leave 10 minutes 5 In a sterile environment lift head out of ethanol 6 Depress highest piston to allow interior to empty 7 Leave to air dry 8 Replace picking head If contamination is suspected the picking head may occasionally be treated with Genetix Sterilizing Agent K8080 as follows 1 Remove picking head from ClonePix FL and remo...
Page 23: ...king Tubing Picking Tubing X4970 is the 8 silicon tubes that connect between the picking head and the actuator These tubes can be cleaned along with the picking head or can be autoclaved using standard conditions 121 C at 15 psi 103 kPa for 20 minutes Ethanol Wash Bath Nylon Bristle Insert The stainless steel Ethanol Wash Bath Nylon Bristle Insert is filled with 70 ethanol in normal use It can add...
Page 24: ...elies on the presence of non expandable system fluid sterile water between the pumps and the picking pins Only at the tip of the picking pins is a small amount of air default 2µl drawn in from the sterile interior immediately prior to sample collection to separate the sample from the system fluid The system fluid is fed in from a detachable wash bottle and the waste fluid is voided directly to the...
Page 25: ...pared using the cleaning procedures described above A Trypticase Soy Agar plate BD Biosciences 221185 was placed open on the robot bed during a prolonged mammalian cell picking run After 20 minutes the plate was covered removed and replaced with another open plate This was done with a total of 6 plates to cover a 2 hour period The plates were incubated at 37 C for 2 weeks to encourage any potentia...
Page 26: ...side to secure the head Fit the picking tubing to the tube connectors starting at the back and working forward making sure that each tube is fitted to the correct connector Follow the instructions to purge and align the pins Important note When handling the picking head take care that the pins are not knocked Damage to pins may produce unreliable results and can cause serious damage to ClonePix FL...
Page 27: ...diameter Single pin X4962 F2 ClonePix FL colony picking pin 0 70mm diameter Single pin X1036 O Rings for picking pins Pack of 8 X4970 Replacement picking tubing Pack of 8 X4948 Picking Pin Removal Key X3445D Cassette for stacker system X4975F 5L Feed wash bottle fixings X4975W 5L Waste bottle fixings X4976 Nylon bristle ethanol bath insert Optional Extras Code Description SL4950 A01 ClonePix FL Re...
Page 28: ...49 label Sterile azide free 10000U 1ml K8251 CloneDetect anti human detection agent Kappa chain specific 649 label Sterile azide free 10000U 1ml K8255 CloneDetect anti human detection agent IgM specific FITC label Sterile azide free 10000U 1ml K8253 CloneDetect anti human detection agent IgM specific 549 label Sterile azide free 10000U 1ml K8254 CloneDetect anti human detection agent IgM specific ...
Page 29: ...olid media for serum free HEK 293 cells 6 x 90ml K8710 CloneMedia CHO semi solid media for CHO s CHOK1 and DG44 cells 1 x 90ml K8700 CloneMedia CHO semi solid media for CHO s CHOK1 and DG44 cells 6 x 90ml K8740 CloneMedia CHO G glutamine free semi solid media for CHO s CHOK1 and DG44 cells 1 x 90ml K8730 CloneMedia CHO G glutamine free semi solid media for CHO s CHOK1 and DG44 cells 6 x 90ml K8725...
Page 30: ...r base EquiGlass TC treated 1 40 W1155 PetriWell 6 Plate Black with clear base EquiGlass Non TC treated 1 40 W1155BC PetriWell 6 Plate Black with clear base EquiGlass Non TC treated Bar coded 1 40 W1157 PetriWell 6 Plate Black with clear base EquiGlass Collagen coated 1 40 W1159 PetriWell 6 Plate Black with clear base EquiGlass Poly D Lysine coated 1 40 W1500 PetriWell 96 Plate Black with clear ba...
Page 31: ...CaliBeads Fluorescent beads for ClonePix FL 200µm diameter Pan wavelength 100ml K8010 Adherent Cell Picking Reagent Type A 1 x 500ml K8020 Adherent Cell Picking Reagent Type B 1 x 500ml K8030 Adherent Cell Picking Reagent Type C 1 x 500ml K8040 Adherent Cell Picking Reagent Type D 1 x 500ml K8005 Adherent Cell Picking Reagent Test Kit Types A D 4 x 250ml K8100 ProbeClean Fluorescently labeled Anti...
Page 32: ...system Purge the supply line Check Z height of pin Not depositing Pin blocked run Purge option or replace pin Dispense volume too low No liquid flow to Wash Station System fluid supply bottle is empty Check air supply and liquid pipes for kinks Check outputs in configuration are correct consult Genetix Customer Support Wash Station overflowing Check no kinks in tubing to waste bottle Waste bottle ...
Page 33: ...g LEDs located under the source plate being imaged EquiGlass A high quality polymer used as the base material for Genetix PetriWell plates It has excellent properties for optimal fluorescent imaging Halogen dryer Proprietary ultra high temperature dryer used as part of the Pin Sanitise system Image Acquisition The capturing of images using pre defined Acquisition Options Image Archive Default loca...
Page 34: ...e Acquisition Option that will be used for colony detection Normally this is based on white light imaging Prime Probe The type of illumination that will be used for colony detection Normally this will be white light by transillumination trans The Prime Probe is set up by creating a Prime Configuration Process A standard program for ClonePix FL to carry out a task such as a series of similar experi...
Page 35: ...rans is normally used to detect colonies Epi illumination is normally used for configuring the robot and for visualising colonies during picking X Drive Robot axis running from right to left XML Extensible Markup Language A simple text format derived from SGML Originally designed to meet the challenges of large scale electronic publishing XML is also used in the exchange of a wide variety of data ...
Page 36: ...etix 16 18 Germicidal radiation 17 H Head 19 I Initial Operation 19 Installation 11 12 16 Isolator switch 17 20 L Lifting 16 19 20 Liquid spillage 18 M Maintenance 18 Maximum 6 Mechanical interlock 19 Microplates 19 O Operating environment 6 P Pin s 13 15 19 Power outlet 18 Power Up Procedures 20 Pre Power Up Checklist 19 R Remove 18 Reset button 15 Run 17 S Safety 17 18 Setup 20 Setup screen 20 S...
Page 37: ...y any right or license to use develop or otherwise exploit this product commercially and no rights are conveyed to the purchaser to use the product its components or the method for any other purposes including without limitation provision of services to a third party or generation of commercial databases For information concerning the availability of licences for other uses please contact Genetix ...
