GE RPN2512E Product User Manual Download Page 7

5. Setting up the Hybridization 

Oven/Shaker

RPN2510EUM  Chapter 5  Rev C  06/2007 

Fig 1. Diagram of instrument control 
panel
Rotisserie/shaker controls 

1. Rotisserie/shaker ON/OFF button 
2. Rotisserie/shaker speed selector dial 
3. Motor on indicator

Temperature controls 

4. Temperature on/off button 
5. Press to set button 
6. LED temperature display 
7. Temperature selector dial

Note:

 the Oven is fitted with automatic 

digital over-temperature protection (see 
Section 8.1. for more details).

NOTE:

 Always ensure that the weight 

is evenly distributed on both sides 
of the rotisserie. Place an empty 
hybridization bottle into the other side 
of the rotisserie as a counterbalance if 
necessary.

Hybridisation Oven/Shaker

press

to set

Rotisserie

rotisserie

(rev/minute)

5

10

70

15

shake r

(strokes/minute)

8 60

2

30

4

45

6

0

Temperatur e (°C)

on

off

on

off

1

2

3

4

5

6

7

1.

   Remove all packaging and place the Hybridization Oven/Shaker on a level 

working surface, ensuring that there is sufficient room above the instrument to 
allow the door to be opened fully.

2.

   Leave the unit to stand for a minimum of 3 hours. This will allow acclimation to 

the new ambient temperature.

3.

   Plug the female end of the power cable into the Hybridization Oven/Shaker.

4.

   Connect the power cable to a suitably grounded electrical outlet. The correct 

operating voltage of the Hybridization Oven/Shaker is found on the product 
information label on the rear of the instrument.

5.

  Turn the Mains ON/OFF switch (1 on Fig 1) to the ON position.

6.

  The GE Healthcare Hybridization Oven/Shaker is now ready for use.

5.1. Setting the oven temperature

1.

   The Oven temperature controls are located on the right hand side of the control 

panel (numbers 4 to 7 in figure 1).

2.

   To turn the temperature control ON press the on / off button (4). The LED display 

(6) will show the current Oven temperature. 

3.

   To set the temperature, press and hold the press to set button (5) and at 

the same time rotate the temperature selector dial (7) until the required 
temperature is shown on the digital display (6).

4.

   Release the press to set button (5) and the temperature display (6) will revert 

back to the actual Oven temperature.

5.

   The set temperature can be viewed at any time simply by pressing and holding 

the press to set button (6)

6.

  The oven will now automatically heat up to the set temperature.

5.2. Setting up the rotisserie

The rotisserie is installed in the Hybridization Oven/Shaker during transit. To use 
the rotisserie for bottle hybridization, the following procedure should be adopted:

1.

   Lift up the oven door to its fullest extent to allow complete access to the oven 

interior.

2.

   Lift the rotisserie vertically out of the oven. Unpack and place on the bench.

3.

   Place the membranes to be hybridized into the required number of hybridization 

bottles. Using the rotisserie as a stand for the bottles, place the bottles into the 
rotisserie, pushing them down as far as they will go.

Summary of Contents for RPN2512E

Page 1: ...GE Healthcare Amersham Hybridization oven shaker Product User Manual Codes RPN2510E RPN2511E RPN2512E...

Page 2: ...branes into bottles 10 7 2 Hybridization 10 7 3 Membrane washing procedures 11 7 3 1 Radioactive Hybridizations 11 7 3 2 AlkPhos Direct Hybridizations 11 8 Maintenance care cleaning of the oven shaker...

Page 3: ...perty of their respective owners 2003 2007 General Electric Company All rights reserved First published November 2003 All goods and services are sold subject to the terms and conditions of sale of the...

Page 4: ...eed platform shaker for box hybridizations depurination denaturation and neutralization steps The instrument is Economical bottle hybridization minimizes probe volumes reducing reagent volumes and enh...

Page 5: ...been supplied with a cordset and plug Check that the appropriate plug for your electrical supply has been included If there is any doubt consult a qualified electrician and inform your local GE Health...

Page 6: ...rical Nominal Voltage Hertz Amp Product code 230 V 50 Hz 3 1 A RPN2510E 110 120 V 60 Hz 4 A RPN2511E 100 V 50 60 Hz 4 A RPN2512E Each instrument is supplied with 1 rotisserie RPN2514 6 hybridization b...

Page 7: ...n Shaker is found on the product information label on the rear of the instrument 5 Turn the Mains ON OFF switch 1 on Fig 1 to the ON position 6 The GE Healthcare Hybridization Oven Shaker is now ready...

Page 8: ...o use the platform shaker for sandwich box hybridizations the following procedure should be adopted 1 Open the oven door to its fullest extent lift the rotisserie vertically and store in a safe place...

Page 9: ...sufficient prehybridization buffer to ensure that the entire surface of the membrane is covered Recommended volume surface area ratio is given in most protocol booklets Seal the box or bag securely 4...

Page 10: ...se membranes These handling advantages should be considered against the potential loss of sensitivity before use When a hybridization mesh is used in conjunction with the membrane the following proced...

Page 11: ...e bottle and the membrane thoroughly with 2 SSC and discard 2 Perform the following stringency washes in large volumes 100 ml minimum of the following solutions at a rotisserie speed of 8 rpm 2 10 min...

Page 12: ...be used Fitting of non approved parts may affect the performance of the safety features designed into the instrument 8 1 Error codes faults The Oven has built in fault diagnostics If a fault occurs t...

Page 13: ...ecommended hybridization buffer or extend prehybridization time 2 4 Increase number of buffer changes during the washing stage or increase stringency of final wash 3 1 Load extra lanes with control DN...

Page 14: ...078 100 1200 1500 872 50 1100 1000 603 1000 500 564d 900 310 800 281 700 271 600 234 500 194 400 125c 300 118 200 72 100 a not necessarily the largest size fragment possible only the largest that is r...

Page 15: ...RPN910D 10 10 cm 10 sheets RPN1010D 15 20 cm 10 sheets RPN1520B RPN1520S RPN1520N RPN1520T RPN1520D 20 20 cm 10 sheets RPN2020B RPN2020S RPN2020N RPN2002T RPN2020D RPN2020E RPN2020F 22 2 22 2 cm 10 sh...

Page 16: ...109 membrane RPN1633 hybridization RPN1634 Ready To Go Random prime dCTP only 10 ng 1 g 5 minutes 2 109 membrane 27 9240 01 DNA labelling hybridization beads DNA labelling Megaprime Random prime any...

Page 17: ...before Quantification Recommended application system hybridization light output re probing to detection AlkPhos Direct RPN3690 0 06 pg 1 hour 5 days yes no Single copy Southern and Northerns ECL Direc...

Page 18: ...MP 35 43 cm 50 28 9068 48 Hyperfilm MP Enveloped 18 24 cm 50 28 9068 50 Hypercassette cassettes for autoradiography and light detection Size Code standard Code deep 18 24 cm RPN11642 RPN11628 24 30 c...

Page 19: ...bridization buffer Product name Pack size Code Rapid Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM Appendix 6 Rev...

Page 20: ...ray RPN2083 455 255 mm 1 tray RPN2093 Safety Tray Liners to match tray 685 455 mm 25 liners RPN1528 to match tray 685 530 mm 25 liners RPN2048 to match tray 530 330 mm 25 liners RPN2058 to match tray...

Page 21: ...21...

Page 22: ...ealthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan GE Healthcare...

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