GE RPN2512E Product User Manual Download Page 12

RPN2510EUM  Chapter 8  Rev C  06/2007 

12 

8. Maintenance/care/cleaning of 

the oven/shaker

The GE Healthcare Hybridization Oven/Shaker is designed to provide trouble-free 
operation. The base of the oven and the shaker tray act as a spills tray and will 
contain any spillage that occurs during hybridization and washing procedures.

To ensure lasting operation the following instructions should be followed:

ALWAYS DISCONNECT THE HYBRIDIZATION OVEN/SHAKER FROM THE 
ELECTRICAL SUPPLY BEFORE CLEANING OR DRYING THE INSTRUMENT.

1.

   Any leakage from the hybridization bottles or the sandwich boxes should be 

cleaned up immediately. Do not allow any liquids to enter the drive mechanism.

2.

   Wipe away any liquids from inside and outside of the unit using soap and water 

with a soft cloth or sponge.

3.

  Do not allow chemicals to remain on unit surfaces.

4.

  Never clean unit with abrasive pads or cleaners.

5.

  Never clean unit with acetone or chloroform.

Only spare parts supplied or specified by GE Healthcare or its agent should 
be used. Fitting of non-approved parts may affect the performance of the 
safety features designed into the instrument.

8.1. Error codes/faults

The Oven has built in fault diagnostics. If a fault occurs, this system will display 
an error code in the LED display to help the service engineer rectify the problem. 
Please see the table below for details of error codes and other faults. In the 
unlikely event that a problem does occur, note down which code / fault you 
observe and contact your nearest GE Healthcare Office for further assistance. If 
you have your own service personnel available for repair, a comprehensive service 
manual is available on request.

Fault 

Safety system in operation 

Fault displayed by

Temperature probe PT100 reading low 

Automatic over-temp system activated  

LED displays Err and 0.1 alternatively 

Temperature probe PT100 reading high  Automatic over-temp system activated 

LED displays Err and 0.2 alternatively

Triac fault 

Secondary relay control activated 

Top left dot flashes in display 

Rotisserie / shaker motor stalled 

Cuts power to motor 

No movement seen but Rotisserie red 

 

 

indicator light still on

Software crash 

Cuts mains power 

No temperature or motor motor power,  

 

 

all LED’s blank

Summary of Contents for RPN2512E

Page 1: ...GE Healthcare Amersham Hybridization oven shaker Product User Manual Codes RPN2510E RPN2511E RPN2512E...

Page 2: ...branes into bottles 10 7 2 Hybridization 10 7 3 Membrane washing procedures 11 7 3 1 Radioactive Hybridizations 11 7 3 2 AlkPhos Direct Hybridizations 11 8 Maintenance care cleaning of the oven shaker...

Page 3: ...perty of their respective owners 2003 2007 General Electric Company All rights reserved First published November 2003 All goods and services are sold subject to the terms and conditions of sale of the...

Page 4: ...eed platform shaker for box hybridizations depurination denaturation and neutralization steps The instrument is Economical bottle hybridization minimizes probe volumes reducing reagent volumes and enh...

Page 5: ...been supplied with a cordset and plug Check that the appropriate plug for your electrical supply has been included If there is any doubt consult a qualified electrician and inform your local GE Health...

Page 6: ...rical Nominal Voltage Hertz Amp Product code 230 V 50 Hz 3 1 A RPN2510E 110 120 V 60 Hz 4 A RPN2511E 100 V 50 60 Hz 4 A RPN2512E Each instrument is supplied with 1 rotisserie RPN2514 6 hybridization b...

Page 7: ...n Shaker is found on the product information label on the rear of the instrument 5 Turn the Mains ON OFF switch 1 on Fig 1 to the ON position 6 The GE Healthcare Hybridization Oven Shaker is now ready...

Page 8: ...o use the platform shaker for sandwich box hybridizations the following procedure should be adopted 1 Open the oven door to its fullest extent lift the rotisserie vertically and store in a safe place...

Page 9: ...sufficient prehybridization buffer to ensure that the entire surface of the membrane is covered Recommended volume surface area ratio is given in most protocol booklets Seal the box or bag securely 4...

Page 10: ...se membranes These handling advantages should be considered against the potential loss of sensitivity before use When a hybridization mesh is used in conjunction with the membrane the following proced...

Page 11: ...e bottle and the membrane thoroughly with 2 SSC and discard 2 Perform the following stringency washes in large volumes 100 ml minimum of the following solutions at a rotisserie speed of 8 rpm 2 10 min...

Page 12: ...be used Fitting of non approved parts may affect the performance of the safety features designed into the instrument 8 1 Error codes faults The Oven has built in fault diagnostics If a fault occurs t...

Page 13: ...ecommended hybridization buffer or extend prehybridization time 2 4 Increase number of buffer changes during the washing stage or increase stringency of final wash 3 1 Load extra lanes with control DN...

Page 14: ...078 100 1200 1500 872 50 1100 1000 603 1000 500 564d 900 310 800 281 700 271 600 234 500 194 400 125c 300 118 200 72 100 a not necessarily the largest size fragment possible only the largest that is r...

Page 15: ...RPN910D 10 10 cm 10 sheets RPN1010D 15 20 cm 10 sheets RPN1520B RPN1520S RPN1520N RPN1520T RPN1520D 20 20 cm 10 sheets RPN2020B RPN2020S RPN2020N RPN2002T RPN2020D RPN2020E RPN2020F 22 2 22 2 cm 10 sh...

Page 16: ...109 membrane RPN1633 hybridization RPN1634 Ready To Go Random prime dCTP only 10 ng 1 g 5 minutes 2 109 membrane 27 9240 01 DNA labelling hybridization beads DNA labelling Megaprime Random prime any...

Page 17: ...before Quantification Recommended application system hybridization light output re probing to detection AlkPhos Direct RPN3690 0 06 pg 1 hour 5 days yes no Single copy Southern and Northerns ECL Direc...

Page 18: ...MP 35 43 cm 50 28 9068 48 Hyperfilm MP Enveloped 18 24 cm 50 28 9068 50 Hypercassette cassettes for autoradiography and light detection Size Code standard Code deep 18 24 cm RPN11642 RPN11628 24 30 c...

Page 19: ...bridization buffer Product name Pack size Code Rapid Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM Appendix 6 Rev...

Page 20: ...ray RPN2083 455 255 mm 1 tray RPN2093 Safety Tray Liners to match tray 685 455 mm 25 liners RPN1528 to match tray 685 530 mm 25 liners RPN2048 to match tray 530 330 mm 25 liners RPN2058 to match tray...

Page 21: ...21...

Page 22: ...ealthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan GE Healthcare...

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