Rehydrate the
Immobiline DryStrip
Follow the instructions below to rehydrate the Immobiline Drystrip.
Action
Step
Prepare the rehydration solution and rehydrate the Immobiline DryStrip in
the strip holder or in the rehydration tray when using the manifold.
1
Follow the recommended procedures found in the Instructions attached to
each Immobiline DryStrip package.
2
See also the 2-D Electrophoresis, Principles and Methods guide containing
commonly used recipes.
Note:
Pipet the rehydration solution
WARNING
Avoid spilling liquids on the body of the instrument. If large volumes
of liquid have penetrated the casing of the instrument and come
into contact with the electrical components, immediately switch
off the instrument and contact an authorized service technician.
CAUTION
Always wear gloves when handling IPG strips and the equipment
that comes in contact with them. This will help minimize protein
contamination which can result in artifactual spots in stained 2-D
gel patterns.
NOTICE
Use the appropriate rehydration volume for the IPG strip length.
Follow the instructions below to pipet the appropriate volume of rehydration solution
into each holder. The correct volume to each strip length is found in the instructions at-
tached to each Immobiline DryStrip package.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
49
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
