5.5
Connecting a packed column
Introduction
This section outlines how to connect a packed column to a BioProcess system in
preparation for performing a run.
The procedure used to perform the run itself depends on a number of factors,
such as the media used, the intended purpose, the process environment and
local regulations.
Note:
Runs are performed in accordance with the standard operating procedures of the cus-
tomer. Contact your GE Healthcare representative if you require assistance in developing
standard operating procedures for your media.
Preparations
When a column has been packed and satisfactory HETP test results have been obtained,
the column is ready for use.
Before a run can be performed the packed column must be disconnected from the
priming and packing equipment. At this stage the column may also be moved, if required,
for connection of MPT and MPB to a BioProcess system.
NOTICE
Check that tubing and connection diameters are compatabile
before connecting a column to a BioProcess system.
To avoid reduced chromatography performance, keep tubing connections as
short as possible when connecting a column to a BioProcess system.
Tip:
Connect a packed column
without bypass lines/valves
Follow the instruction below to connect a pre-packed column without bypass lines/valves
to a BioProcess system.
Action
Step
Connect tubing to the system valve marked COLUMN BOTTOM, but do not
connect the other end of the tubing to the bottom of the column at this time.
1
Set the system column valves to the UpFlow position.
2
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LPLC and MPLC Columns Operating Instructions 28-9638-66 AC
5 Operation
5.5 Connecting a packed column
