6.2.3
Preparing a sample for densitometry
Introduction
This section describes how to prepare a densitometry sample for scanning.
Prepare the sample
The illustrations under the Densitometry tab in the main window of the
Amersham Typhoon Control Software provide instructions for placing gels, spacers and
the digitization plate. The instructions provided in the software are adapted to the den-
sitometry method that is chosen.
For best accuracy of OD measurements, it is recommended to measure relative
values in the range 0 to 1 and limit the scan area to 10 × 10 cm (mini-gel size).
It is not recommended to use relative OD values above 1 for quantitation. It is
not recommended either to compare relative OD values between gels.
Tip:
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Amersham Typhoon Operating Instructions 29193226 AC
6 Operation
6.2 Preparing the sample
6.2.3 Preparing a sample for densitometry
