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GE Amersham Imager 680, Operating Instructions Manual

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GE Amersham Imager 680 Operating Instructions Manual Download Page 109

5.5

Capture an image

This section describes how to capture an image with any of the available methods.

In this section

See page

Section

110

5.5.1 Available methods

111

5.5.2 Chemiluminescence capture

137

5.5.3 Colorimetric capture

141

5.5.4 Fluorescence capture

Amersham Imager 680 Operating Instructions 29271308 AB

109

5 Operation

5.5 Capture an image

Summary of Contents for Amersham Imager 680

Page 1: ...Amersham Imager 680 Operating Instructions Original instructions Translation disc enclosed...

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Page 3: ...Feature overview 42 3 3 Illustration of the instrument 43 3 4 Instrument hardware description 50 3 5 Instrument software description 51 3 5 1 Description of the Capture tab 56 3 5 2 Description of the...

Page 4: ...5 Normalization 196 5 8 6 Summary 199 5 9 Turn off the instrument 200 6 Analysis software 201 6 1 Software installation and activation 202 6 1 1 Amersham Imager 680 Analysis Software installation 205...

Page 5: ...247 9 2 Health and Safety Declaration Form 249 Index Amersham Imager 680 Operating Instructions 29271308 AB 5 Table of Contents...

Page 6: ...Page intentionally left blank...

Page 7: ...afety notices regula tory information and a general description of the intended use of Amersham Imager 680 In this chapter See page Section 8 1 1 About this manual 9 1 2 Important user information 11...

Page 8: ...in a safe way Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware...

Page 9: ...ystem that produces digitial images of samples in gels or membranes in three different modes chemiluminescence colorimetric and fluorescence Amersham Imager 680 is intended for research use only and s...

Page 10: ...s situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instruction...

Page 11: ...rement GE Healthcare Bio Sciences AB Name and address of manufacturer Bj rkgatan 30 SE 751 84 Uppsala Sweden In this section See page Section 12 1 3 1 EU Directives 13 1 3 2 Eurasian Customs Union 14...

Page 12: ...tic Compatibility EMC Directive 2014 30 EU Low Voltage Directive LVD 2014 35 EU Restriction of Hazardous Substances RoHS Directive 2011 65 EU CE marking The CE marking and the corresponding EU Declara...

Page 13: ...tion Requirement See Manufacturing information Name and address of manufacturer Telephone 46 771 400 600 Telephone number of manufacturer GE Healthcare LLC Importer and or company for obtain ing infor...

Page 14: ...r s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are design...

Page 15: ...environnement 1999 Notification English Notification about this product Hg Contains mercury For safe handling procedures and the measures to be taken in case of accidental breakage and for options ava...

Page 16: ...et le recyclage veuillez consulter le site https www ec gc ca mercure mercury Default asp lang Fr n DB6D2996 1 Pour obtenir une aide suppl mentaire veuillez communiquer avec votre repr sentant GE Ce...

Page 17: ...lectrical equipment for mea surement control and laboratory use Part 1 General requirements EN 61010 1 IEC 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 Particular requirements for laboratory equipment...

Page 18: ...depending on configuration is equipped with some or all light sources in the table below Wavelength Light sources 460 nm Blue LED 520 nm Green LED 630 nm Red LED 470 nm to 635 nm White LED 312 nm Tran...

Page 19: ...nt WARNING Before installing operating or maintaining the product all users must read and understand the entire contents of this chapter to become aware of the hazards involved In this chapter See pag...

Page 20: ...pment in the area The safety precautions in this section are grouped into the following categories General precautions Personal protection Installation Operation Maintenance General precautions WARNIN...

Page 21: ...c shock If the power supply cords are damaged contact your local GE representative for replacements WARNING Access to power switch and power cord with plug Do not block access to the power switch and...

Page 22: ...t open the instrument door during operation when the RUN indicator is lit If the door is opened and the instrument keeps run ning turn off the instrument immediately and contact GE service CAUTION Do...

Page 23: ...ate one WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION...

Page 24: ...ion The gap is small and you may injure your hand CAUTION Always use appropriate Personal Protective Equipment PPE during operation and maintenance of Amersham Imager 680 Protective eyewear Protective...

Page 25: ...e If left these may degrade and cause damage to the instrument CAUTION Do not disable the interlock of the equipment as UV light can be emitted if the door is opened NOTICE Both the Amersham Imager 68...

Page 26: ...ay impair performance Simultaneous use can cause loss of data NOTICE Avoid connecting additional accessories such as computers monitors or keyboards to the instrument during operation as this can stop...

Page 27: ...system use a monitor with SVGA resolution With a low resolution monitor operation buttons cannot be seen NOTICE Remove all traces of acidic solutions to prevent corrosion in the equipment Maintenance...

Page 28: ...el lining CAUTION Turn off the power switch before cleaning the inside of the instru ment CAUTION UV lamps in Amersham Imager configurations 680 UV 680 QC and 680 RGB contain mercury which must be rec...

Page 29: ...erformingmaintenanceoftheequipment removethetablet computer There is risk of injury if it falls on your foot Amersham Imager 680 Operating Instructions 29271308 AB 29 2 Safety instructions 2 1 Safety...

Page 30: ...instrument The system label identifies the product and shows electrical data and regulatory compliance Manufacturing date Manufacturing year and month are determined by the serial number on the system...

Page 31: ...al waste and must be collected separately Please contact an authorized repre sentative of the manufacturer for information concerning the de commissioning of equipment This symbol indicatesthat the pr...

Page 32: ...stration number of the product will appear beside the symbol This equipment has been tested and found to comply with the limits for a Class A digital device Position of label and additional symbols 32...

Page 33: ...iption on page 43 Note Description Symbol On Off switch Power switch I O I Power ON O Power OFF Connector for DVI D monitor DVI D USB port Ethernet port Caution hot surface Amersham Imager 680 Operati...

Page 34: ...isconnect the power cord from the wall socket Precautions WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be...

Page 35: ...ing to national and local environmental regulations Recycling of hazardous substance The lamps in Amersham Imager 680 configurations 680 QC 680 UV and 680 RGB contain mercury vapour These must be recy...

Page 36: ...procedures depending on what type of environment the unit was located in Follow local and or national federal requirements for disposal of the Amersham Imager 680 Cabinet and the system cabinet 2 Dis...

Page 37: ...s so that the use of such electrical and electronic products will not result in any severe environmen tal pollution any bodily injury or damage to any assets The unit of the period is Year In order to...

Page 38: ...2 0 Indicates that this hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in GB T 26572 X Indicates that this hazardous substance contained...

Page 39: ...changes to the software the screen captures in this manual may differ sligtly compared to the acutal software However the general functions are the same Note In this chapter See page Section 40 3 1 In...

Page 40: ...on mem branes obtained by electrophoresis or western blotting separation methods The instru ment can capture images of chemiluminescent fluorescent and colorimetric samples depending on the system co...

Page 41: ...lor image of a chemiluminescence sample image and a white light image of a color marker Fluorescence imaging using blue green and red Epi light sources and UV trans illu minator Color images of colori...

Page 42: ...connected tablet computer It may also be operated from a wired touchscreen or a monitor with mouse The illustration below displays Amersham Imager 680 setup with tablet computer 42 Amersham Imager 68...

Page 43: ...urations Amersham Imager 680 RGB 29270772 Amersham Imager 680 QC 29270771 Amersham Imager 680 UV 29270770 Amersham Imager 680 29270769 Functions X X X X Control Software for image capture and analysis...

Page 44: ...USB port 1 On Off button Press the button to turn on power Press again to turn off the power On Off button and LED indicators 2 Power The LED is lit white when the instru ment is powered on Run The L...

Page 45: ...ad Air attachment iPad Air can be placed on the dock Instrument door Door 4 Toggle switch Power switch 5 I Power ON O Power OFF Connector for attaching the AC power cord Power connector 6 Port for con...

Page 46: ...position the tray Lower Tray guide 2 White light Trans White light source 3 UV light Trans UV light source 4 For configurations 680 680 UV and 680 QC White light source Epi light source 5 For configu...

Page 47: ...Power on Steady light X X Busy Run Flashing 1 Hz X X Error ON 0 5 s OFF 0 5 s Flashing 1 Hz X Shutdown ON 0 5 s OFF 0 5 s Flashing slowly 0 5 Hz X Power save ON 0 5 s OFF 1 5 s Mount iPad Air attachme...

Page 48: ...guide until you hear a clicking sound 2 Connect an iPad Air to the attachment 3 Removing the attachment Action Step Push the center of the attachment to release the lock points 1 48 Amersham Imager 68...

Page 49: ...Action Step Pull the attachment downward 2 Amersham Imager 680 Operating Instructions 29271308 AB 49 3 Instrument description 3 4 Instrument hardware description...

Page 50: ...digital vi sual interface connected touchscreen or a monitor plus mouse Note The available functions may vary depending on the system configuration Note For details on system configurations see Secti...

Page 51: ...describes the basic elements of the Capture tab 10 1 2 6 5 4 3 7 8 9 Function Part The selected image capturing method 1 Capture The tab for selecting image capturing method and starting ex posure 2...

Page 52: ...re proceeding Method sensitive displays The display on the screen shows the selected image capturing method and presents the options relevant for that method Depending on system configuration you may...

Page 53: ...xposure drop down menu use to select the exposure option for chemilu minescence image capturing Auto Semi Auto Manual Incremental and Avanced Note For more information on manual and incremental settin...

Page 54: ...turing method 2 Epi illumination selected here Trans illumination Fluorescence The illustration below shows the specific components of the Capture tab with the Fluo rescence image capturing method Dep...

Page 55: ...Auto selected here Semi Auto Manual Advanced Note For more information on manual settings see Set exposure time on page68 High dynamic range checkbox Use the check box for image capture with high dyn...

Page 56: ...The selected file location here On instrument use to view file storage options for example USB flash drive or network locations Select the drop down menu for more file location options 2 Search field...

Page 57: ...all Deselect all button use the buttons to select all files displayed or to deselect a selection of files 10 Delete button use the button to delete the selected marked file or files 11 Rename button u...

Page 58: ...9 Function Part Display of the captured image Note Incremental and Advanced exposures may result in several images being displayed two at a time as they are exposed Navigate among the images using fo...

Page 59: ...109 If the colorimetric marker option was checked the tabs will display Sample and Marker If a multichannel fluores cence capture was per formed the tabs will dis play the selected channels of Blue G...

Page 60: ...play is the default view 5 Done button Use the button to leave the view without saving Note A dialog appears asking to confirm the cancellation This prevents deleting images by accident 6 Save button...

Page 61: ...use the button to print the image Note The print function requires that the instrument is connected to a USB con nected printer For installation of a printer contact a representative from GE 9 Amersha...

Page 62: ...For more details see Sec tion 5 8 Analyze the image on page 181 Description The following illustration shows the start screen and first step of the analysis 1 2 3 4 5 6 Function Part Contrast button...

Page 63: ...pen the lane creation step with the button 1 Lane Creation is used to define and position lanes to be used in the subsequent analysis Open the background subtraction step with the button 2 Background...

Page 64: ...utton 4 MW Calibration is used to create a molecular weight calibrationcurve based on a selected standard marker Open the normalization step with the button 5 Normalization is used to select bands to...

Page 65: ...resent an overview of the result data from the analysis Use the Save button to save the analysis data together with the image file Amersham Imager 680 Operating Instructions 29271308 AB 65 3 Instrumen...

Page 66: ...urn to the previous view 1 Network button Use to set up DHCP or Static IP network 2 Show mouse cursor check box Use to display a mouse cursor on a con nected external monitor by checking the check box...

Page 67: ...ent or Rename The following illustration and table show the main components of the keypad The image to the left shows the alphabetic view of the keypad with lower case letters The image to the right s...

Page 68: ...keypad 11 123 button Use the button to change to the numeric and symbol keypad Note Use abc or the ABC button to return to the alphabetic keypad 12 Set exposure time The Set exposure time view appear...

Page 69: ...he exposure time by Hours Minutes Seconds or 1 10 sec 3 Keypad Use the right and left arrow buttons to navigate between the Hours Minutes Seconds and 1 10 sec fields The active field appears blue in t...

Page 70: ...Description Part All configurations 29083417 Black tray marked Black Tray 680 UV 29083419 UV Trans tray marked UV Trans Tray 680 QC 680 RGB transparent 680 QC 29083418 White Trans tray marked White Tr...

Page 71: ...rating Instructions All configurations 29272133 DVD containing translations of Amersham Imager 680 Operating Instructions All configurations 29272479 Amersham Imager 680 Analysis Software All configur...

Page 72: ...iPad Air attach ment Upgrade options Relevant for con figuration Code no Part Description 600 680 29083422 AI 600 680 upgrade to UV 600 680 UV 29083424 AI 600 680 upgrade UV to QC 600 680 QC 29083425...

Page 73: ...ort of the Amersham Imager 680 instrument must be performed by GE authorized personnel The instrument contains parts that may be damaged unless they are secured for transportation and handled correctl...

Page 74: ...space required around the imager 10 cm free space on all other sides 980 N 100 kg or higher Load capacity Horizontal 2 Inclination of bench surface WARNING Access to power cord Do not block access to...

Page 75: ...should not be exposed to sources of heat such as radiators or direct sunlight Dust in the atmosphere should be kept to a minimum The equipment must not be excessively exposed to vibration or impacts E...

Page 76: ...om with a temperature above 28 C Higher temperatures does not allow the CCD to cool down properly to 25 C For environmental requirements see Environmental requirements on page 245 76 Amersham Imager 6...

Page 77: ...Imager 680 must be secured before long distance transports Contact GE for instructions before transport or relocation of the instrument For connecting additional monitors or peripheral equipment conta...

Page 78: ...tings view Open the settings view by selecting the settings icon found in the top right hand corner of the Capture and Library tabs For the general components of the Settings view see Section 3 5 5 De...

Page 79: ...h and day 2 Time field Use the plus and minus buttons next to Hour and Minute to set values for hour and minute 3 Ok button Use the button to save the changes to date and time and return to the Settin...

Page 80: ...ield to change the value of hour or minute Save the changes to date and time Select the Ok button to save the changes to the Date time view Select the Cancel button to leave the view without saving ch...

Page 81: ...cus value 3 Single arrow buttons Use the buttons to adjust focus in smaller steps 4 Default button Use the button to reset focus to the default value Note The default value differs between instruments...

Page 82: ...arrows above and below the Focus value 4 Select OK to save the settings Note Cancel will close the view without saving changes 5 The new focus will be available for captures until a new capturing met...

Page 83: ...same location It identifies which instrument is connected to a certain tablet computer Select the Instrument indicator button This activates the Run LED light on the instrument it is connected to Ame...

Page 84: ...Follow the instructions below to update the software for Amersham Imager 680 Action Step Connect a USB flash drive containing the update file provided by GE stored at the root level of the USB flash...

Page 85: ...Action Step Select the Export log button 1 Result A text file will be created on the instrument containing the export log file Insert a USB flash drive and confirm insertion by selecting the OK button...

Page 86: ...ng steps Action Step In the Settings menu click Log in 1 In the Log in window click On 2 Enter a password and click Done Note The default password is AI600user 3 86 Amersham Imager 680 Operating Instr...

Page 87: ...Step In the Settings menu click Log in 1 To change password click the Change Password button 2 Enter current password new password confirm new password then click OK 3 Amersham Imager 680 Operating I...

Page 88: ...the following steps Action Step In the Settings menu click Log in 1 To turn off log in functionality select Off Enter the current password and click Done to turn it off 2 88 Amersham Imager 680 Operat...

Page 89: ...and delete image files from a computer This section contains information on network setup options virus protection network settings and file access In this section See page Section 90 4 4 1 Network se...

Page 90: ...t connect telephone lines to the Ethernet port Only the IEC 60950 I VL 60950 1 standard non shielded cables are appropriate for connection to this connector Connection to a network enables the instrum...

Page 91: ...ppear Select the Network button 2 Result The Network view will appear Select the type of network connection and follow the separate instructions below 3 Connect to a DHCP network Follow the instructio...

Page 92: ...ge 67 3 Select OK 4 Result An automatic connection to the DHCP network is established within a couple of seconds Tip Reserve the IP address to Amersham Imager 680 for easier access of its net work res...

Page 93: ...d to enter the subnet mask If desired select the Gateway field and use the keypad to enter the router Workgroup field and use the keypad to enter the workgroup Select OK to save the changes 4 Result T...

Page 94: ...the network identification name 1 Create a new folder and name the folder 2 Example AmershamImager680 Right click on the folder and select Properties Share with 3 Select which user or group you wish t...

Page 95: ...sharing is turned on you must have a user account and password on your computer for full access to shared items Password protected sharing is located in the Control panel under Advanced sharing settin...

Page 96: ...e Network view Open the view by selecting the settings icon in the top right hand corner and then select the Network button in the Settings view DHCP networks has a dynamic IP address assignment there...

Page 97: ...s displayed Note The password can be changed Click Option in the Login window and follow the instruction in the Options window Type the default password AI600user and select Login 3 Result This opens...

Page 98: ...x Note A maximum number of 5 files can be copied at one time You may repeat this step to copy additional files 5 Select the Copy button to copy the selected files Select the Delete button to delete th...

Page 99: ...fety precautions on page 20 for details WARNING Do not operate Amersham Imager 680 in any other way than de scribed in the User Manual In this chapter See page Section 100 5 1 Operation flow chart 101...

Page 100: ...Description Stage Turn on the instrument 1 Select a tray and place sample 2 Place the tray in the instrument 3 Capture the image 4 Save the image file 5 Analyze the image 6 100 Amersham Imager 680 Ope...

Page 101: ...nt of the instrument to activate it 2 Result The instrument s self diagnostic process will initialize Note The self diagnostic process will normally take approximately 5 minutes Connection to a networ...

Page 102: ...e connected tablet computer 4 Result 1 A welcome screen appears 2 The following screen will be displayed 3 If Log in is turned On then password prompt is displayed on welcome screen 102 Amersham Image...

Page 103: ...Action Step Select the Continue button 5 Result The following screen will be displayed Amersham Imager 680 Operating Instructions 29271308 AB 103 5 Operation 5 2 Turn on the instrument...

Page 104: ...Action Step Wait for the CCD status to turn green 6 Result Image capturing can begin 104 Amersham Imager 680 Operating Instructions 29271308 AB 5 Operation 5 2 Turn on the instrument...

Page 105: ...680 Black Tray Membrane Gel Colorimetric Epi illumination 680 UV 680 QC White Insert or White Trans Tray 680 RGB 680 QC White Trans Tray Gel Colorimetric Trans illumination 680 RGB Diffuser Board 680...

Page 106: ...t tray position in Amersham Imager 680 Action Step Select a tray and accessories suitable for the capturing method 1 For Chemiluminescence with colorimetric marker and Colorimetric Epi capturing place...

Page 107: ...f view 1 One side of the White Insert indicates the maximum field of view of 8 x 11 cm for the upper tray position Use the upper tray position for Chemiluminescence capturing for a smaller field of vi...

Page 108: ...ortant to avoid capturing artefacts in the image Open the instrument door 4 Insert the tray in the correct tray position Note For Chemiluminescence capturing you may also select the upper tray positio...

Page 109: ...of the available methods In this section See page Section 110 5 5 1 Available methods 111 5 5 2 Chemiluminescence capture 137 5 5 3 Colorimetric capture 141 5 5 4 Fluorescence capture Amersham Imager...

Page 110: ...n x x Colorimetric Trans illumination x x x Fluorescence UV trans illumination x Fluorescence Blue Green Red Epi illumination single channel or multichannnel x Fluorescence Blue Green Red Epi illumina...

Page 111: ...pturewithcolorimet ric markers is performed on one to four mem branes placed on a Black Tray with a White In sert Note The White Insert has 2 sides one for upper tray position and the other for the lo...

Page 112: ...he sample To use the full dynamic range of the image file check High dynamic range To minimize exposure time leave High dynamic range unchecked Select Semi automatic A specific area of a sample is of...

Page 113: ...es to capture se quential images A sequence of images is required Select Advanced exposure Full control of exposure parameters is required for special applications Select Change and choose desired bin...

Page 114: ...n the Capture tab select Chemiluminescence 1 Result The options available for Chemiluminescence capturing are displayed to the right on the screen Automatic exposure is selected by default and the Exp...

Page 115: ...tray is inserted in lower or upper position 2 Lower position Upper position Choose the desired binning option and click OK If your sample contains a colorimetric marker then select the check box Color...

Page 116: ...ill begin following a pre exposure A progress bar appears on the screen indicating the time remaining of the imaging process the exposure selected and the calculated remaining time Note Selecting Canc...

Page 117: ...ages are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant tab for the image you wish to display A selected tab is highlighted with blue text and frame Select bot...

Page 118: ...If your sample contains a colorimetric marker then select the check box Colorimetric marker 2 To change Binning use the Change button 3 To use the full dynamic range of the image file check High dyna...

Page 119: ...ose to zoom in Choose to zoom out Use the contrast tool and arrows to get an optimal view of the area of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed image i...

Page 120: ...se then select one point of interest in the image Result The selected point is highlighted with an X Choose and then select two diagonal corners of an area of interest in the image Result The selected...

Page 121: ...are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant button for the image you wish to display A selected tab is highlighted with blue text and frame Select both...

Page 122: ...options available for chemiluminescence capturing are displayed to the right on the screen If your sample contains a colorimetric marker then check the check box Colorimetric marker 2 Select Manual ex...

Page 123: ...he new time and select Set to return to the previous view For detailed instructions see Section 3 5 5 Description of settings views on page 66 6 Select Start and wait for the exposure to finish 7 Amer...

Page 124: ...ges are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant tab for the image you wish to display A se lected tab is highlighted with blue text and frame Select bot...

Page 125: ...g are displayed to the right on the screen Select Incremental exposure in the exposure drop down menu 2 Set the number of repetitive exposures in the Expose field using the plus and minus buttons 3 Ch...

Page 126: ...Set Interval time by pressing the value 7 Result This opens the Set interval time view Enter the value and select Set to to save the value and to return to the pre vious view 8 The Reset button resets...

Page 127: ...ar indicates the time remaining of the imaging process When the exposure is finished the image view appears displaying the images You can navigate among the images by selecting the left or right arrow...

Page 128: ...ce capture with Advanced exposure mode This exposure mode is for greater control of image capture in case it is needed for a particular application Note NOTICE When using advanced exposure images that...

Page 129: ...ending on the conditions The table below shows the available combinations for the lower tray position No colorimetric marker Colorimetric marker Lower Lower Tray position 4 levels 4 levels Binning 4 a...

Page 130: ...inning setting 4 A pop up window displays the binning options Note The Binning function increases sensitivity by binning pixels and results in lower image resolu tion 130 Amersham Imager 680 Operating...

Page 131: ...r tray position with standard sensitivity without colorimetric maker 5 Set the number of repetitive exposures in Number of captures by using the plus and minus buttons 6 Amersham Imager 680 Operating...

Page 132: ...iew The Reset button resets the value to zero Set the exposure time by pressing value 8 Result the Set exposure time view opens Enter the value and select Set to save the value and to return to the pr...

Page 133: ...Action Step Select Start exposure 9 Amersham Imager 680 Operating Instructions 29271308 AB 133 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture...

Page 134: ...hlighted with blue text and frame Select both buttons simultaneously to see a composite color image of the Sample and Marker images When the exposure is finished the image view appears dis playing the...

Page 135: ...o be saved then Save to save the images If Colorimetric marker was selected it is possible to check the check box With marker to display the marker image Note If more than one image is selected then t...

Page 136: ...Image tools on page 164 for further instructions To analyze the image select Analyze See Section 5 8 Analyze the image on page 181 for further instructions To print the image select Print To discard...

Page 137: ...ource at the bottom of the instrument interior When using Trans illumination the intensity data is converted to optical density OD values which provides a more quantitative result compared to Epi illu...

Page 138: ...ow the instructions in the table to perform colorimetric capture Action Step In the Capture tab select Colorimetric 1 Result The options available for colorimetric capturing are displayed to the right...

Page 139: ...his is important to avoid artefacts in the image 2 Note 1 For Trans illumination place a diffuser board at the bottom of the instrument with the matte side up before capturing Select Start 3 Result A...

Page 140: ...fter capturing To save the image select Save This will allow you to reopen the image later to use the image tools to perform an analysis or to print the image See Section 5 7 Save and manage image fil...

Page 141: ...x x x Trans UV The Custom method allows for different combinations of the blue green or red light sources and an emission filter of choice Note Fluorescence capture options The table below illustrate...

Page 142: ...ynamic range unchecked Select Semi automatic A specific area of a sample is of interest A short pre exposure is performed which results in an image This allows you to select the area of the sample ima...

Page 143: ...p In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the right on the screen To use the full dynamic range of the image file check High d...

Page 144: ...to the next step For Advanced exposure see Fluorescence RGB with Advanced exposure on page 151 If exposure option Manual was selected then select the Exposure time Set button 5 Note If more than one...

Page 145: ...Set Result This will close the view For details see Set exposure time on page 68 Select Start A progress bar appears on the screen indicating the time re maining of the imaging process and the select...

Page 146: ...is highlighted with blue text and frame Select two or more tabs simultaneously to see a composite color image Twodetectionchan nels were selected Select the relevant tab of Blue Green or Red for the...

Page 147: ...ure with a colorimetric marker Action Step In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the right on the screen Check the Epi RGB r...

Page 148: ...ure on page 151 If Auto or Manual exposure was chosen select Start 5 Result A progress bar appears on the screen indicating the time remaining of the imaging process and the selected exposure option P...

Page 149: ...to get an optimal view of the area of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed image indicated by the blue line in the image histogram Choose red arrows...

Page 150: ...re and wait for the exposure to finish 9 When the exposure is finished the image view appears displaying the images Amersham Imager 680 creates a folder containing the created image files which can be...

Page 151: ...dexposure mode imagesthatare not selected will be deleted at saving Action Step In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the ri...

Page 152: ...an be specified depending on the conditions The table below shows the available combinations No Colorimetric marker Colorimetric marker Lower Lower Tray position 3 levels 3 levels Binning 4 areas 1 ar...

Page 153: ...position Note The Binning increases sensitivity by binning pixels and results in lower image resolution Select an area in Image area This is only available for image capture in the lower tray positio...

Page 154: ...aiting time by pressing value 7 Result this opens the Set waiting time view Enter the value and select Set to save the value and to return to the previous view The Reset button resets the value to zer...

Page 155: ...exposure time view opens Enter the value and select Set to save the value and to return to the previous view The Reset button resets the value to zero Amersham Imager 680 Operating Instructions 292713...

Page 156: ...Action Step Select Start exposure 9 156 Amersham Imager 680 Operating Instructions 29271308 AB 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture...

Page 157: ...utton is highlighted with blue text and frame Select both buttons simultaneously to see a composite color image of the Sample and Marker images When the exposure is finished the image view appears dis...

Page 158: ...t to select the images to be saved then Save to save the images Note If more than one image is selected then the Library view will open after selecting Save Fluorescence Custom The Custom option is de...

Page 159: ...ansparent tray 2 Place the sample on the gel sheet 3 Proceed with the capturing Note After use wash the gel sheet with mild detergent rinse with water then dry well A gel sheet can be reused about 20...

Page 160: ...only effective with Auto and Semi Auto exposure setting If Auto or Manual exposure was selected select Start 5 Result A progress bar appears on the screen indicating the time remaining of the imaging...

Page 161: ...Choose to zoom in Choose to zoom out Use the contrast tool and arrows to get an optimal view of the area of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed imag...

Page 162: ...orners of an area Result The selected area is highlighted with a square Use the right hand side arrow buttons to move the highlighted point or area Click Start exposure and wait for the exposure to fi...

Page 163: ...f the image view on page 58 1 2 3 4 Function Part The captured image is displayed 1 Tabs for captured images Use the tab to select or deselect images of the different detection channels Note A display...

Page 164: ...und and white bands Use the Rotate 90 degrees left or right buttons to rotate the image Rotation changes will be saved in the image file Use the Edit image buttons to edit the image into separate imag...

Page 165: ...tion plotting the number of pixels for each intensity value in the captured image The horizontal axis represents inten sity variations The vertical axis represents the number of pixels with a given in...

Page 166: ...able tool Navigate between the images using the forward and backward arrows Point to a specific area of interest to dis play the maximum pixel intensisty and the average pixel intensity in the area Ti...

Page 167: ...an access and manage files stored on the network location This section describes the locations where you can save files how to save a file the various file formats and the file management options NOTI...

Page 168: ...ble providing the instrument has been granted access to the network location For more details see Section 4 4 Network on page 89 Note Connect a USB flash drive A USB flash driver is automatically inst...

Page 169: ...Location field Note The network settings need to be properly configured before a network folder can be added as a location for saving files For information on how to configure a network see Section 4...

Page 170: ...Edit save location This table describes how to edit a location for files saving Action Step Select the settings icon in the top right hand corner of the screen 1 Select the Save locations button 2 Se...

Page 171: ...Action Step To save the changes select the OK button 6 Amersham Imager 680 Operating Instructions 29271308 AB 171 5 Operation 5 7 Save and manage image files 5 7 1 Save locations...

Page 172: ...ion Step Select Save to save the image or images 1 Result The Save view opens The automatically generated image name s are displayed in the lower part of the view Note 172 Amersham Imager 680 Operatin...

Page 173: ...e instrument memory status is full the oldest file will automatically be deleted when a new image is saved It is therefore recommended to save the images on a USB flash drive or a network drive It is...

Page 174: ...age captures and analyses have been completed for the day If the Amersham Imager 680 instrument is used around the clock make sure to restart the system at least once a day to remove temporary files t...

Page 175: ...trast for presentation purposes Note The jpg image is included in the folder but it is not displayed on the screen with colorimetric marker A folder containing the following files Colorimetric Sample...

Page 176: ...jpg composite im age of fluorescence and colorimetric data in color and with set contrast Note The composite image is displayed if both tabs are selected simultaneously A jpg of the Marker image in co...

Page 177: ...for quantifica tion of fluorescence data Note The number of tif files created correspond to the number of selected detection chan nels Blue Green Red A jpg composite image of fluorescence data in colo...

Page 178: ...he files in the library Delete files Follow the instruction to delete files from the device Action Step Select the file s to delete 1 Select the Delete button 2 Result The following window will appear...

Page 179: ...nd move files Action Step Select the file s to copy or move 1 Select the Copy Move button 2 The following window will appear Select the location to copy or move the file s to in the drop down menu in...

Page 180: ...pad to change the name of the file Select Done to save the changes 3 For detailed information on how to use the keypad see Keypad view on page 67 Open files Follow the instruction to open files Action...

Page 181: ...alysis results The workflow is performed in five steps and the final result is presented in a summary You can analyze a newly captured image or analyze a previously saved image Select the Analyze butt...

Page 182: ...ion Function Part The current step in the analysis workflow highlighted 1 Back button Use the button to close the analysis workflow 2 182 Amersham Imager 680 Operating Instructions 29271308 AB 5 Opera...

Page 183: ...Zoom buttons Use the button to zoom in on or zoom out of the image Navigation arrow buttons Use the button to move the image on the screen The automatically created lane grid and lane numbers 4 The di...

Page 184: ...lane grid to correspond with the lanes in the image Adjust the lane grid Follow the instructions below to adjust the lane grid to match lanes in the image Action Step Select the minus and plus buttons...

Page 185: ...ns adjacent to Lane width to increase or decrease the lane fill factor Note If the value is 100 then the grid contains only the lanes Using a lesser per centage creates an empty space between lanes 4...

Page 186: ...racts background for all lanes in the image Note Use the Show background check box to see how each background subtraction options works This function shows the background level calculated by each opti...

Page 187: ...will subtract more background than a large Rolling ball Subtract background Action Step If an image of the sample is displayed select the lane profile button 1 Result A screen will appear displaying...

Page 188: ...d radio buttons 3 None Rubber band Minimum profile Rolling ball and then select the Radius plus or minus buttons to set the radius for the virtual rolling ball eraser to set the new baseline of the gr...

Page 189: ...result in any lane select the lane number to view in the drop down menu When you are done select the Next button or select the Bands button in the workflow The Previous button will bring you back to...

Page 190: ...e image or lane profile according to the detection channel used For example a band detected in the green channel is displayed with a green frame etc Detecting bands Follow the instructions below to de...

Page 191: ...To shift a band position select the band and shift the band position up or down with the Position arrow buttons To adjust a band width select the band and adjust it with the Width plus and minus butto...

Page 192: ...lecular weight calibration can only be performed in single channel view Note Action Step Select the lane containing the molecular weight marker 1 Result The selected lane appears highlighted Select th...

Page 193: ...vious button will bring you back to the previ ous step 7 The calculated molecular weight is presented in the summarized table in Summary Note Addamolecularweightstandard marker Action Step Select the...

Page 194: ...Type a new value and select the Add button 3 Result The new value will appear in the list Note You may need to scroll to see the new value Select the Save button to save the edited values and to retur...

Page 195: ...e selected band volumes Normalization is useful when comparing relative band volumes in different lanes The options for normalization are presented next to the image Normalize bands Follow the instruc...

Page 196: ...tton is highlighted in the workflow The illustration and table below describe the summary view 1 2 3 4 Function Part The analyzed image 1 Tabs for displaying details 2 Print button Use to print the su...

Page 197: ...lysis is completed click Done 2 Result The image view appears To save the analysis click Save 3 Result The image file is updated with the analysis information Choose a location to save the analysis by...

Page 198: ...analyzed data is saved with a CSV file and the summary view is saved as a pdf file See Section 5 7 Save and manage image files on page 167 for details 198 Amersham Imager 680 Operating Instructions 29...

Page 199: ...of the instrument to the O position 2 Result The instrument is turned off To optimize the instrument s life time and to minimize unnecessary power consumption shut down the instrument when image captu...

Page 200: ...ftware can be installed on computers using Windows 7 Windows 8 1 or Windows 10 operating system The Amersham Imager 680 Analysis Software can be used with images generated by both the Amersham Imager...

Page 201: ...ware installation is performed in the following sequence 1 Install the Amersham Imager 680 Analysis Software 2 A registration number is presented by the Amersham Imager 680 Analysis Software 3 Go the...

Page 202: ...llShieldWizard dialog box appears Click Next 3 Read the license agreement If the license agreement is not acceptable please contact your GE representative 4 If the license agreement is acceptable sele...

Page 203: ...am Files x86 is acceptable click Next If you want to use a different folder click Change and define a suitable destination folder Click Next Click Install 7 Amersham Imager 680 Operating Instructions...

Page 204: ...ick Finish in InstallShield Wizard 8 204 Amersham Imager 680 Operating Instructions 29271308 AB 6 Analysis software 6 1 Software installation and activation 6 1 1 Amersham Imager 680 Analysis Software...

Page 205: ...he Reg istration dialogue box 1 Make a note of the 4 digit registration number for later use Note 3216 is an example This 4 digit number will differ each time you install the software 2 Note Access to...

Page 206: ...alysis software license appears Enter the Serial number and the Registration number Note The 6 digit serial number is printed on the analysis software DVD 4 206 Amersham Imager 680 Operating Instructi...

Page 207: ...ars 6 Result The 10 digit License key number is generated Make a note of the 10 digit License key number Amersham Imager 680 Operating Instructions 29271308 AB 207 6 Analysis software 6 1 Software ins...

Page 208: ...mber and the License key to the registration screen 7 208 Amersham Imager 680 Operating Instructions 29271308 AB 6 Analysis software 6 1 Software installation and activation 6 1 2 Amersham Imager 680...

Page 209: ...re is now complete Note The software license screen shows the number of licenses generated Amersham Imager 680 Operating Instructions 29271308 AB 209 6 Analysis software 6 1 Software installation and...

Page 210: ...to perform a step by step analysis of the image data and to save the results in the same way as with the Image analysis function in the Amersham Imager 680 Control Software Action Step The workflow s...

Page 211: ...ge file opens in the analysis workflow The following illustration and table describe the start view of the analysis workflow 1 2 3 4 5 6 7 8 Function Part The current step in the analysis workflow is...

Page 212: ...button Use to access the image tools which modify display settings and to view image details 3 Display Zoom Contrast Intensity For more information see Image tools on page 164 212 Amersham Imager 680...

Page 213: ...h a blue frame 5 Options and steps associated with the current step in the analysis 6 Help icon Use to show additional information about the selected step in the analysis 7 Next button Use to proceed...

Page 214: ...grid to correspond with the lanes in the image Adjust the lane grid Follow the instructions below to adjust the lane grid to match lanes in the image Action Step Select the minus and plus buttons adj...

Page 215: ...adjacent to Lane width to increase or decrease the lane fill factor Note If the value is 100 then the grid contains only the lanes Using a lesser per centage creates an empty space between lanes 4 Whe...

Page 216: ...n option works This function shows the background level calculated by each option Tip The illustration and tables below describe the background subtraction options Description Option Use this option w...

Page 217: ...i mum profile Use this option to subtract the background below the graph as if erasing it using the radius of a ball A small radius will subtract more background than a large Rolling ball Amersham Ima...

Page 218: ...e current lane number displayed in the drop down list and the lane profile button in blue To confirm that each background subtraction options works check Show background check box This function shows...

Page 219: ...dius plus or minus buttons to set the radius of the virtual rolling ball eraser Tip The smaller the radius the more background will be removed Result The lane profile view is displayed with the backgr...

Page 220: ...mber in the drop down menu 4 When ready click Next or select the Bands in the workflow Clicking Previous takes you back one step 5 220 Amersham Imager 680 Operating Instructions 29271308 AB 6 Analysis...

Page 221: ...ge or lane profile according to the detection channel used For example a band detected in the green channel is displayed with a green frame etc Detecting bands Follow the instructions below to detect...

Page 222: ...ift a band position select the band and shift the band position up or down with the Position arrow buttons To adjust a band width select the band and adjust it with the Width plus and minus buttons To...

Page 223: ...weight calibration can only be performed in single channel view Note Action Step Select the lane containing the molecular weight marker 1 Result The selected lane appears highlighted Select the stand...

Page 224: ...revious button will bring you back to the previ ous step 7 The calculated molecular weight is presented in the Summary table Note Addamolecularweightstandard marker Action Step Select the Add new butt...

Page 225: ...a new value and select the Add button 3 Result The new value will appear in the list Note You may need to scroll to see the new value Select the Save button to save the edited values and to return to...

Page 226: ...lected band volumes Normalization is useful when comparing relative band volumes in different lanes The options for normalization are presented next to the image Normalize bands Follow the instruction...

Page 227: ...is highlighted in the workflow The illustration and table below describe the summary view 1 2 3 4 Function Part The analyzed image 1 Tabs for displaying details 2 Print button Use to print the summar...

Page 228: ...ane the relative band volume within a lane in percentage Normalized value the band volume relative to the control band volume Mw the calculated Mw Rf the relative mobility of the band Finalize and sav...

Page 229: ...mage as view appears Select Create a folder and type a file name then click Save 4 Result In addition to the image file s analyzed data is saved with a CSV file and the summary view is saved as a pdf...

Page 230: ...erformed by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Do not use excessive amounts of liquids for cleaning...

Page 231: ...or 70 ethanol then wipe with a new clean dry cloth To clean the interior of the instrument use a soft cloth slightly dampened with distilled water or 70 ethanol Make sure to wipe the interior dry Do...

Page 232: ...id that residues fluoresce when images are captured The accessories may be washed with water and one of the following 70 ethanol Mild neutral detergent Isopropanol 6 H2O2 Cleaning sample trays white i...

Page 233: ...er using a soft sponge soaked in a cleaning agent After cleaning rinse the accessory immediately in water until no detergent remains and allow to air dry Cleaning gel sheets After use wash the gel she...

Page 234: ...plug is firmly secured in the power outlet The power cord and supply plug do not become overheated The power cord is not damaged in any way The ventilation holes in the equipment are free from dust an...

Page 235: ...image quality 240 8 3 Problems with the software General troubleshooting procedure Follow this procedure if an error occurs 1 Take note of the error code and error message on the monitor 2 Turn off th...

Page 236: ...hen a camera head failure has occurred Turn off the power switch immediately and contact your GE representative Atypical odors are emitted then an instru ment failure has occurred Smoke is emitted The...

Page 237: ...losed or the door cannot be locked because a foreign object is present in the locking section or the door sensor The locking section is damaged Contact your GE representative The door to the instrumen...

Page 238: ...he light source are dirty Change to the correct sample tray The wrong sample tray is used Select the correct light source The wrong light source is selected Place the sample correctly on the sample tr...

Page 239: ...strument and contact your GE representa tive A lamp in the UV transil luminator is broken Noise appears in an image exposed using the UV Trans tray Then If The UV Trans Tray is contaminated Clean the...

Page 240: ...ion prop erly Check the sample position The sample is not in position The screen of Amersham Imager 680 is not displayed on the connected tablet computer Then If Contact the network administrator or c...

Page 241: ...ew minutes for the instrument to come online The wireless network can be detected before the instrumented is fully connected Normally the instrument becomes online within 5 minutes Restart the softwar...

Page 242: ...page to the home screen of the tablet computer by clicking on the square with an upward arrow next to the address field in the browser Tip If a USB dongle is used on the camera the default IP address...

Page 243: ...hapter also includes ordering information and Health and Safety Declaration form for service In this chapter This chapter contains the following sections See page Section 244 9 1 Specifications 247 9...

Page 244: ...nation light 470 to 635 nm Fully automated auto exposure no focus or other adjustment or calibrations needed Operation 160 X 220 mm Maximum sample size 65 536 levels 16 bit Greyscale 1 100 second to 1...

Page 245: ...680 CAUTION Do not use the instrument in a room with a temperature above 28 C Higher temperatures does not allow the CCD to cool down properly to 25 C Requirement Parameter Temperature 18 C to 28 C w...

Page 246: ...into consideration the workflow and ancillary facilities when planning the installation 2 Any required construction and electricity air conditioning work must be completed prior to installation 3 It...

Page 247: ...orothersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperatingloc...

Page 248: ...hazardous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youfor ad...

Page 249: ...7 M Maintenance 84 230 Amersham Imager 680 231 Sample trays and filter 233 Manufacturing information 11 Monitor requirements 27 Moving instrument 77 Multichannel imaging 143 N Notes and tips 10 O On s...

Page 250: ...pad 67 Space requirements 74 Specifications 244 Standards 17 Start screen 51 T Tool Web 96 Tools Contrast tool 165 Display tool 164 Intensity tool 166 Zoom tool 164 Troubleshooting 235 Two channel ima...

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Page 252: ...care which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzin...

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