GE AKTApure, User Manual, Page: 91 / 522

3.2.1 Function
Introduction
Fraction collector F9-C can collect fractions in deep well plates, tubes of different sizes or bottles. Up
to six cassettes for deep well plates and tubes can be used. The cassettes can be used in any combi-
nation and are placed on the Cassette tray. A rack for 50 ml tubes and a rack for 250 ml bottles are
also available.
Scanner functions detect which types of trays, racks, cassettes and deep well plates that are used in
each run.
The Cassette tray or one of the racks is placed inside the fraction collector. A height exclusion bar en-
sures that the bottles, tubes and deep well plates are correctly positioned and cannot damage the
Dispenser head. The Tray catch and positioning discs on the floor of the fraction collector guide the
Cassette tray or the rack into the correct position.
Scanning of Cassettes
When the door of the fraction collector is closed automatic scanning is performed. There are two types
of scanning procedures:
• Full scan: Scanning of Cassette type codes to determine which types of Cassettes are used, and
scanning of rows and columns in deep well plates to identify which types of plates are used (24,
48, or 96 wells). Full scan is performed only when the system is in state Ready.
• Quick scan: Scanning of Cassette type codes to determine which type of Cassettes are used. Quick
scan is performed during the run to ensure that correct Cassettes are placed in the Fraction collector.
Fractionation modes to avoid spillage
Three fractionation modes are available, all of which avoid spillage between wells or tubes during
fractionation:
• Accumulator: The accumulator is used to collect liquid during movement between wells, tubes or
bottles. The liquid is then dispensed in the next well or tube. Fractionation with accumulator can
be used at all flow rates.
• DropSync: When using DropSync the sensors in the Dispenser head detect when a drop is released
from the nozzle. The Dispenser head moves to the next well or tube just after a drop is released.
Fractionation with DropSync can be used at flow rates up to 2 ml/min. If the cassettes are placed
near the waste funnel the DropSync mode can be used at higher flow rates. Volatile solutions and
solutions with low surface tension may require a lower flow.
• Automatic: The fraction collector uses the Drop Sync mode for flow rates up to 2 ml/min and auto-
matically switches to Accumulator mode for higher flow rates.
ÄKTA pure User Manual 29119969 AB 91
3 ÄKTA pure external modules
3.2 Fraction collector F9-C
3.2.1 Function