5.4
Connect a column
Introduction
This section describes how to connect a column to the instrument using a column
holder and without introducing air into the flow path. Several types of column holders
are available for ÄKTA pure.
WARNING
To avoid exposing the column to excessive pressure, make sure
that the pressure limit is set to the specified maximum pressure of
the column. Before connecting a column to the ÄKTA pure instru-
ment, read the instructions for use of the column.
Methods automatically include a pressure alarm based on the specifications of the
chosen column type. However, when running manual runs you have to set the pressure
limits yourself. Also, to protect the column media, special settings are needed. See
tion 5.5 Pressure alarms, on page 114
for more information on pressure alarms.
Do not overtighten when connecting columns. Overtightening might rupture
the connectors or squeeze the tubing and thereby result in high back pressure.
Note:
If no column valve is used, remove the column from the system before running
a system wash. The pressure during a system wash may become too high for
the column.
Note:
ÄKTA pure Operating Instructions 29022997 AF
109
5 Prepare the system for a run
5.4 Connect a column
