GE AKTA avant, User Manual

Page: 433 / 538

Tubing types
The table below shows the tubing types used in ÄKTA avant.
Volume/cm Scope of use Color Description
0.5 μl High pressure tubing
Reference capillary 1 Blue PEEK, o.d. 1/16", i.d. 0.25
mm
2.0 μl High pressure tubing Orange PEEK, o.d. 1/16", i.d. 0.50
mm
4.4 μl High pressure tubing Green PEEK, o.d. 1/16", i.d. 0.75
mm
7.8 μl High pressure tubing Beige PEEK, o.d. 1/16", i.d. 1.0
mm
20.0 μl Inlet tubing Transpar-
ent FEP, o.d. 1/8", i.d. 1.6
mm
66.0 μl Inlet tubing for high flow
rate and high viscosity Transpar-
ent FEP, o.d. 3/16", i.d. 2.9
mm
4.4 μl Narrow inlet tubing (op-
tional) Transpar-
ent ETFE, o.d. 1/16", i.d. 0.75
mm
7.8 μl Outlet and waste tubing Transpar-
ent ETFE, o.d. 1/16", i.d. 1.0
mm
35.0 μl Pump rinse solution
tubing Transpar-
ent Silicone, o.d. 4.1 mm, i.d.
2.1 mm
0.5 ml Waste tubing from
Buffer tray Transpar-
ent Silicone, o.d. 12 mm, i.d.
8 mm
Many different sizes/types of tubing can be connected to a chromatography
system. Tubing with a smaller inner diameter (i.d.) holds less delay volume and
will therefore generate less dilution of the protein peak. Narrow tubing, how-
ever, increases the system pressure, especially when running at high flow
rates. The tubing used should match the application needs. Note:
Tubing connectors
The table below shows the tubing connectors used in ÄKTA avant.
Use with tubing... Description
PEEK and ETFE with o.d. 1/16" Fingertight connector, 1/16"
ÄKTA avant User Manual 29035184 AE 433
9 Reference information
9.3 Tubing and connectors