7.4 Connect a column
Introduction
This section describes how to connect a column to the instrument, using a column holder
and without introducing air into the flow path. Several types of column holders are
available for ÄKTAavant.
WARNING
Before connecting a column to the ÄKTAavant instrument, read
the instructions for use of the column. To avoid exposing the column
to excessive pressure, make sure that the pressure limit is set to
the specified maximum pressure of the column.
Methods automatically include a pressure alarm based on the tolerance of the chosen
column type. However, when running manual runs you have to set the pressure limits
yourself.
CAUTION
Do not overtighten when connecting columns. Overtightening might
rupture the connectors or squeeze the tubing and thereby result
in high backpressure.
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
83
7 Prepare the system for a run
7.4 Connect a column
Summary of Contents for AKTA avant
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