GE AKTA avant, Getting Started

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6.1.3 Predefined phases
The predefined phases that are available in the predefined methods and in the Phase
Library are described in the table below.
Description Phase
The first, and mandatory, phase in any method. Defines
common parameters used in the subsequent phases. Method Settings
Equilibrates the column before purification, or re-equilibrates
the column after purification. Equilibration
Applies sample to the column. Defines the sample application
technique, the sample volume, and the handling of
flowthrough. Sample Application
Washes out unbound sample after sample application or
removes strongly bound proteins after elution. Column Wash
Elutes the sample from the column. Defines parameters for
the elution and fractionation settings. Elution
Prepares the column before use by removing the storage
solution and equilibrating the column. By adding steps, several
preparation solutions can be used sequentially. Column
Preparation
Cleans the column after purification runs by rinsing the column
with a cleaning solution to remove unspecifically bound
proteins. By adding steps, several cleaning solutions can be
used sequentially. Column CIP
Prepares the system before a run by removing storage solution
and filling the system and inlets with buffer solution. One
preparation solution is used per phase. System
Preparation
Cleans the system after purification runs by rinsing the system
with a cleaning solution. One cleaning solution is used per
phase. System CIP
Tests the efficiency of a packed column in terms of height
equivalent to a theoretical plate (HETP), and the peak
asymmetry factor (A
s
).
Column
Performance Test
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB 67
6 Create a method
6.1 Guide to method creation
6.1.3 Predefined phases