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Function

Part

Function

Part

Start pre-capture

13

Help

6

Start image capture

14

Show or hide Advanced set-
tings

7

Select dyes

Filter

Wavelength (nm)

Dye

Cy3(UV)

365

UV

Cy2

460

Cy2

Cy3(UV)

535

Cy3

Cy5

653

Cy5

IRshort

660

IRshort

IRlong

775

IRlong

To select which dye or dyes are being used in the sample, use the following steps. A
maximum of three dyes can be analyzed at one time.

Action

Step

To select the dyes used, under 2.Basic settings, tap Select.

1

68

Amersham ImageQuant 800 User Manual 29383813 AA

3 Handle methods in ImageQuant 800 control software

3.6 Fluorescence image capture
3.6.1 Fluorescence imaging

Summary of Contents for 800

Page 1: ...Amersham ImageQuant 800 User Manual ...

Page 2: ...Automatic chemiluminescence exposure 53 3 4 3 Manual chemiluminescence exposure 55 3 4 4 Time series chemiluminescence exposure 58 3 4 5 SNOW chemiluminescence exposure 61 3 5 Colorimetric image capture 62 3 5 1 Colorimetric imaging 64 3 5 2 Gel documentation 65 3 5 3 Optical Densitometry 66 3 6 Fluorescence image capture 67 3 6 1 Fluorescence imaging 70 3 6 2 Automatic fluorescence exposure 73 3 ...

Page 3: ...101 6 2 Image problems 103 6 3 Software problems 104 6 4 Error messages 106 Index Amersham ImageQuant 800 User Manual 29383813 AA 3 Table of Contents ...

Page 4: ...nt user information and a general description of the Amersham ImageQuant 800 and its intended use In this chapter See page Section 5 1 1 About this manual 7 1 2 The Amersham ImageQuant 800 instrument 4 Amersham ImageQuant 800 User Manual 29383813 AA 1 Introduction ...

Page 5: ...ant 800 Operating Instructions Scope of this manual The User Manual covers the Amersham ImageQuant 800 instrument Amersham ImageQuant 800 control software and Amersham ImageQuant CONNECT The illustration below shows the Amersham ImageQuant 800 system Depending on your system configuration not all options described in this manual may be available It is possible to upgrade your system to increase th...

Page 6: ... menu Hardware items are identified in the text by bold text for example Power Notes and tips A note is used to indicate information that is important for trouble free and optimal use of the product Note A tip contains useful information that can improve or optimize your procedures Tip 6 Amersham ImageQuant 800 User Manual 29383813 AA 1 Introduction 1 1 About this manual ...

Page 7: ...be analyzed with standard image analysis software such as GE Healthcare ImageQuant analysis software Contact your GE Healthcare representative for more information regarding analysis software The instrument is designed to be used for research purposes in life science laboratories within academia and industry Instrument configuration The Amersham ImageQuant 800 is available in several different con...

Page 8: ...r 525BP20 Cy2 605BP40 Cy3 UV 705BP40 Cy5 715BP30 IRshort 836BP46 IRlong The Amersham ImageQuant 800 instrument can be further equipped with customemissionfilters seeAmershamImageQuant800OperatingInstructions Note The figure below illustrateshow these LED light sources and emission filters cover different wavelength ranges of the light spectrum 8 Amersham ImageQuant 800 User Manual 29383813 AA 1 In...

Page 9: ...ow to start and set up the Amersham ImageQuant 800 control software and Amersham ImageQuant CONNECT software See page Section 10 2 1 Amersham ImageQuant 800 control software 29 2 2 Amersham ImageQuant CONNECT Amersham ImageQuant 800 User Manual 29383813 AA 9 2 System Preparation ...

Page 10: ... control software adjust settings and manage user accounts In this chapter See page Section 11 2 1 1 Introduction 12 2 1 2 Start the Amersham ImageQuant 800 control software 16 2 1 3 System settings 22 2 1 4 Account management 10 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation 2 1 Amersham ImageQuant 800 control software ...

Page 11: ...the touchscreen panel or an external monitor For information on the installation process and how to set up the Amersham ImageQuant 800 system refer to the Amersham ImageQuant 800 Operating Instructions Amersham ImageQuant 800 User Manual 29383813 AA 11 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 1 Introduction ...

Page 12: ...he power button on the right side of the instrument to activate it 2 Result The instrument s self diagnostic process will initialize Note The self diagnostic process will normally take a few minutes Turn on the computer and log into Windows Note The Amersham ImageQuant 800 control software will auto start after Win dows login 3 Tap I Agree to agree to the End User License Agreement Note Uncheck th...

Page 13: ...apturing can begin Start the system using an external monitor It is also possible to start the system via the external PC if an external monitor is being used To do this use the following procedure Action Step Make sure that the instrument is plugged in to a mains power supply 1 Amersham ImageQuant 800 User Manual 29383813 AA 13 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1...

Page 14: ...ger Turn on the computer and log into Windows 3 Double click on the Amersham ImageQuant 800 control software icon to connect to the instrument 4 If Login is turned On enter your Username and Password Note To enable or disable Log in refer to Turn off password protection on page22 5 14 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation 2 1 Amersham ImageQuant 800 control software ...

Page 15: ...ep mode The Login screen will display If password protection has been turned off the Home screen will display The default time until the instrument enters sleep mode is 30 minutes To change this setting refer to the Amersham ImageQuant 800 Operating Instructions Note Amersham ImageQuant 800 User Manual 29383813 AA 15 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 2 Start the...

Page 16: ...fixed distance For thick samples however or when imaging multi well plates or petri dishes the focus might need to be adjusted The image below shows the Focus view in the system settings To adjust the focus for a specific imaging mode follow the steps below Action Step Open the imaging mode being used Note In this example Chemiluminescence imaging mode is used 1 Open the Settings tab 2 Select Focu...

Page 17: ...der in the Brightness box 3 to change the brightness of the image 5 Tap Save to save the focus settings and return to the Chemiluminescence screen Note Focus changes will only affect the selected image capture mode If the user changes to another mode for example Fluorescence the focal distance will still be set to the default setting 6 Amersham ImageQuant 800 User Manual 29383813 AA 17 2 System Pr...

Page 18: ...our system config uration these dye settings for each filter are available for selection within the Amersham ImageQuant 800 control software Additional dye settings can be added in the Settings panel For more informationon which filters are supplied with which system configuration see the Amersham ImageQuant 800 Operating Instructions To add a new light filter combination for a fluorescent dye to ...

Page 19: ...ed focal distance 6 Select whether the Correction is calculated automatically or manually apply a set correction file 7 Note For more information on correction file see Image correction files on page20 Tap Save 8 Amersham ImageQuant 800 User Manual 29383813 AA 19 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 3 System settings ...

Page 20: ...s select the dye to be deleted by tapping the checkbox 1 Tap Delete 2 Tap OK 3 Image correction files Image correction files are used to correct image non uniformity in CCD cameras Correc tion files are provided for each light source These are applied automatically to each light source but can also be set manually by the user when a dye is created see Add a light filter combination on page 18 New ...

Page 21: ...ave locations 1 Tap Add location 2 In the file browser navigate to the desired folder and tap Select 3 Tap Save 4 To make the folder the default save location tap the check box next to the folder name and tap Make default 5 Result the default symbol will appear next to the selected folder Amersham ImageQuant 800 User Manual 29383813 AA 21 2 System Preparation 2 1 Amersham ImageQuant 800 control so...

Page 22: ...nstru ment is installed Turn off password protection By default the Amersham ImageQuant 800 control software is password protected It is possible to turn off the password requirement for the Amersham ImageQuant 800 by disabling the System login To do this use the following procedure Action Step In the Settings screen tap User accounts on the left side of the screen 1 Next to System login settings ...

Page 23: ...OFF 4 Close the System user login ON OFF window by tapping the cross in the top right corner 5 Result the System login settings now shows as OFF Tap Done 6 Amersham ImageQuant 800 User Manual 29383813 AA 23 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 4 Account management ...

Page 24: ...ftware Create a new user New users can be added in the Settings screen To do this use the following steps Action Step In the Settings screen tap User accounts on the left side of the screen 1 Tap User accounts on the left side of the screen 2 24 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 4 Account management ...

Page 25: ... again in the Confirm password space 6 Tap Add 7 Tap Done 8 Delete a user To delete a user account use the following steps Account deletion can only be performed by an administrator with the master password Note Amersham ImageQuant 800 User Manual 29383813 AA 25 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 4 Account management ...

Page 26: ...tap OK 6 Note This action cannot be undone after tapping OK If an account is deleted in error an new account can be made See Create a new user on page 24 Change a password To change the password of the current user follow the procedure below Action Step In the Settings screen tap User accounts on the left side of the screen 1 26 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation ...

Page 27: ...rent password for the current user 3 Enter a New password for the user 4 Type the password again in the Confirm password space 5 Tap Add 6 Tap Done 7 Amersham ImageQuant 800 User Manual 29383813 AA 27 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 4 Account management ...

Page 28: ...owing steps Action Step Tap the cogwheel icon in the top left corner and tap Settings 1 Tap the box next to the desired user to select that account 2 Tap Reset password 3 Enter the master password and tap OK 4 Result the user s password is reset to 0123456789 Tap Save 5 28 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation 2 1 Amersham ImageQuant 800 control software 2 1 4 Accoun...

Page 29: ...ect to and manage Amersham ImageQuant 800 instruments via the Amersham ImageQuant CONNECT software package In this chapter See page Section 30 2 2 1 Introduction 31 2 2 2 Connect to the instrument Amersham ImageQuant 800 User Manual 29383813 AA 29 2 System Preparation 2 2 Amersham ImageQuant CONNECT ...

Page 30: ... DVD To install Amersham ImageQuant CONNECT insert the DVD and run the installation file ImageQuant CONNECT Home screen The image below shows the Amersham ImageQuant CONNECT Home screen Function Part List of available Amersham ImageQuant 800 instruments 1 Name of the active user 2 Sync instruments 3 Options to open Settings Logout from the software show software version information or show the Lic...

Page 31: ...Quant CONNECT software use the following steps Action Step Click the options icon in the top right corner and click Settings 1 Click Add instrument 2 Result a new line appears on the Instruments list Amersham ImageQuant 800 User Manual 29383813 AA 31 2 System Preparation 2 2 Amersham ImageQuant CONNECT 2 2 2 Connect to the instrument ...

Page 32: ...r network name of the instrument 4 Click Save 5 Log in to ImageQuant CONNECT To log in to Amersham ImageQuant CONNECT and access the Amersham ImageQuant 800 from a remote computer use the following procedure Action Step Double click on the Amersham ImageQuant CONNECT icon 1 Carefully read the license agreement and click I Agree 2 32 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparat...

Page 33: ...een shows Note To refresh the status of the connected instruments click Sync Select the instrument to connect 4 Result the selected instrument Image Library screen shows Amersham ImageQuant 800 User Manual 29383813 AA 33 2 System Preparation 2 2 Amersham ImageQuant CONNECT 2 2 2 Connect to the instrument ...

Page 34: ...e following steps Action Step Click the Instrument drop down menu at the top of the screen 1 Click the instrument to which you want to connect 2 Sync instruments To refresh the list of connected Amersham ImageQuant 800 instruments click the refresh button at the top of the screen 34 Amersham ImageQuant 800 User Manual 29383813 AA 2 System Preparation 2 2 Amersham ImageQuant CONNECT 2 2 2 Connect t...

Page 35: ...top right corner and click Settings 1 Click the box next to the instrument to be deleted 2 Click Delete 3 Click Save Note To undo the deletion click Cancel This must be done before the Save button is clicked 4 Amersham ImageQuant 800 User Manual 29383813 AA 35 2 System Preparation 2 2 Amersham ImageQuant CONNECT 2 2 2 Connect to the instrument ...

Page 36: ...am ImageQuant 800 control software In this chapter See page Section 37 3 1 Introduction 38 3 2 General settings 41 3 3 Advanced settings 45 3 4 Chemiluminescence image capture 61 3 5 Colorimetric image capture 66 3 6 Fluorescence image capture 36 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software ...

Page 37: ...chemilumines cence colorimetric and fluorescence The main functions and uses of these modes are described in the table below Sample type Imaging options Image capture mode Enzymatically labelled membranes e g horseradish peroxidase Chemiluminescence Chemiluminescence Colorimetric markers e g Amersham Rainbow Markers With colorimetric marker Colorimetric stained gels e g Coomassie and silver staini...

Page 38: ... can be placed in the upper 1 or lower 2 tray position The position of the tray is detected automatically by the Amersham ImageQuant 800 control software and displayed at the bottom of the screen The display is automatically updated if the tray position is changed 38 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 2 General settings ...

Page 39: ...Multiple exposures are captured and merged by averaging the results into a single image This improves the signal to noiseratio by removing artefacts and reducing false positive signals This setting can be used for Chemiluminescence and Fluorescence imaging methods Amersham ImageQuant 800 User Manual 29383813 AA 39 3 Handle methods in ImageQuant 800 control software 3 2 General settings ...

Page 40: ...1 In the Methods window tap to select the method and tap Select 2 Result the image capture method screen is shown Tap Start 3 Help function For information tips and help when using the ImageQuant 800 control software tap the Help button in the top right corner 40 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 2 General settings ...

Page 41: ...m ImageQuant 800 has a maximum image capture area of 160 220 mm This capture area is dependent on which tray position is being used and whether the NP lens is being used The capture area under each setting is detailed in the table below Capture area Tray position 80 110 mm Upper 160 220 mm Lower 133 183 mm 107 147 mm 80 110 mm 146 161 mm NP lens for plates Amersham ImageQuant 800 User Manual 29383...

Page 42: ...the tabs on the right side to select the desired capture area 2 160 220 mm 133 183 mm 107 147 mm 80 110 mm Result the capture area is changed to the selected size Note Changing the capture area is only possible in the Lower tray position In the Upper tray position it is possible to see a live view of the capture area to po sition the sample within the default capture area Tap Select 3 42 Amersham ...

Page 43: ...xels are grouped into one pixel This allows for detection of a fainter signal in image analysis but results in a loss of resolution compared to 2 2 binning When performing image capture on the Amersham ImageQuant 800 it is possible to adjust the binning settings for either greater sensitivity or greater resolution Increasing the sensitivity results in a decrease in the exposure time The option for...

Page 44: ...move the slider towards High resolution or High sensitivity 2 Tap Select 3 To avoid image saturation either set a lower binning or reduce the exposure time Tip 44 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 3 Advanced settings ...

Page 45: ...ee page Section 46 3 4 1 Chemiluminescence imaging 50 3 4 2 Automatic chemiluminescence exposure 53 3 4 3 Manual chemiluminescence exposure 55 3 4 4 Time series chemiluminescence exposure 58 3 4 5 SNOW chemiluminescence exposure Amersham ImageQuant 800 User Manual 29383813 AA 45 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture ...

Page 46: ... are needed for chemilumines cence imaging Imaging mode Black tray Without colorimetric marker Black tray with White insert With colorimetric marker The Chemiluminescence screen The illustration below shows the main components of the Chemiluminescence screen Function Part Function Part Toggle full dynamic range 12 Save image capture settings as a method 1 Image library tab 13 SNOW noise reduction ...

Page 47: ...o perform sequential chemiluminescence and fluorescence imaging without moving the sample Up to two fluorescence dyes can be imagedin additionto thechemiluminescenceimaging This makes furtherdownstream processing of the images easier The option to add multiplex imaging is found in the Advanced settings panel when creating a method To use the fluorescence multiplex imaging use the following steps A...

Page 48: ...me manually under Exposure time To add a second dye tap Add dye 5 To remove a dye tap the cross on that dye 6 Tap Select Note To cancel changes and return to the previous screen tap Cancel 7 48 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture 3 4 1 Chemiluminescence imaging ...

Page 49: ... following procedure Action Step Place the tray guide inside the black tray 1 Place the multi well plate or petri dish into the cutout in the centre of the tray guide 2 Open the instrument door 3 Insert the tray into the lower tray position 4 Insert the NP lens into the lower tray position on top of the tray Note The NP lens must be inserted fully up to the stop position 5 Adjust the focus if need...

Page 50: ...lowing steps Action Step On the Home screen tap Chemiluminescence image capture 1 Under 1 Select Exposure tap Auto 2 If an overlay image of the sample and marker is required tap the toggle button next to Colorimetric marker 3 If shorter exposure times are required tap the toggle button next to Full dynamic range to the off position 4 Note Turning off Full dynamic range will reduce the range of int...

Page 51: ...e To take an automatic exposure without predefining a region of interest tap Start without tapping Pre capture 8 Tap the button to toggle between Point and Area measurement 9 Amersham ImageQuant 800 User Manual 29383813 AA 51 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture 3 4 2 Automatic chemiluminescence exposure ...

Page 52: ... reference toggle to Area Tap and drag to select the desired reference area 1 Note The area can be moved without redrawing it by using the arrow buttons 2 Tap Start 11 52 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture 3 4 2 Automatic chemiluminescence exposure ...

Page 53: ...ure time tap the box above hour min sec 1 10 sec 3 In the Exposure time window tap the box under hour min sec or 1 10 sec and use either and buttons or the keypad to enter the desired value 4 Note The maximum time that can be set is 10 hours The minimum time that can be set is 0 1 sec Tap OK to return to the create method window 5 Amersham ImageQuant 800 User Manual 29383813 AA 53 3 Handle methods...

Page 54: ...page 41 for more information 7 Enter the settings for Fluorescence multiplex if required see Fluorescence multiplex on page 47 8 To save the capture settings for future use tap Save method In the popup window enter the Name of the method and tap Save 9 Tap Start 10 54 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image cap...

Page 55: ...e sample Cumulative Image exposure will stop as soon as regions start becoming saturated In a time series exposure cumulative imaging will also stop when saturation has been reached This means that subsequent images will not be taken Stop when saturated Take a time series exposure To take a time series chemiluminescence exposure use the following steps Action Step On the Home screen tap Chemilumin...

Page 56: ...of the sample and marker is required tap the toggle button next to Colorimetric marker 8 Under Advanced settings change the settings for Binning and Capture area as required See Section 3 3 Advanced settings on page 41 for more information 9 To save the capture settings for future use tap Save method In the popup window enter the Name of the method and tap Save 10 56 Amersham ImageQuant 800 User M...

Page 57: ... being taken The current image being taken will show as Exposing and images to be taken afterwards as Pending Amersham ImageQuant 800 User Manual 29383813 AA 57 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture 3 4 4 Time series chemiluminescence exposure ...

Page 58: ... out The Target improvement ratio can be altered during a run or the process can be stopped and the image saved directly if this happens Note Take an image using SNOW To capture an image using the SNOW function use the following steps Action Step On the Home screen tap Fluorescence image capture 1 Under 1 Select Exposure tap SNOW 2 If an overlay image of the sample and marker is required tap the t...

Page 59: ...terest and the selected background area Select a flat background area that is free from bands stain speckles or dust If it is not possible to select such a back ground area for example if a post stained gel exhibits many dye speckles in the gel background it is recommended to select a smaller area or an area next to the gel Amersham ImageQuant 800 User Manual 29383813 AA 59 3 Handle methods in Ima...

Page 60: ...the signal to noise improvement ratio will update To manually enter a target improvement ratio during image capture toggle off Auto stop tap the box next to Target improvement ratio and enter a new value 11 Result the manual boundary will show in the graph 60 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 4 Chemiluminescence image capture 3 4 ...

Page 61: ...create and run colorimetricimagingmethods In this section See page Section 62 3 5 1 Colorimetric imaging 64 3 5 2 Gel documentation 65 3 5 3 Optical Densitometry Amersham ImageQuant 800 User Manual 29383813 AA 61 3 Handle methods in ImageQuant 800 control software 3 5 Colorimetric image capture ...

Page 62: ...k tray with White insert Epi illumination Glass tray Trans illumination To achieve the best possible accuracy trans illumination is recommended This method is only available in certain instrument configurations For more information on instrument configurations and upgrading the Amersham ImageQuant 800 instrument see Amersham ImageQuant 800 Operating Instructions Note The Colorimetric tab The illus...

Page 63: ...hideadvancedsettings 5 OD measurement 2 Start capture 6 Gel documentation 3 Amersham ImageQuant 800 User Manual 29383813 AA 63 3 Handle methods in ImageQuant 800 control software 3 5 Colorimetric image capture 3 5 1 Colorimetric imaging ...

Page 64: ...tric image capture 1 Under 1 Select Exposure tap Gel documentation 2 Under Advanced settings change the settings for Capture area as required See Section 3 3 Advanced settings on page 41 for more information 3 To save the capture settings for future use tap Save method In the popup window enter the Name of the method and tap Save 4 Tap Start 5 64 Amersham ImageQuant 800 User Manual 29383813 AA 3 H...

Page 65: ... image use the following steps Action Step On the Home screen tap Colorimetric image capture 1 Under 1 Select Exposure tap OD measurement 2 Under Advanced settings change the settings for Capture area as required See Section 3 3 Advanced settings on page 41 for more information 3 To save the capture settings for future use tap Save method In the popup window enter the Name of the method and tap Sa...

Page 66: ...aging methods In this section See page Section 67 3 6 1 Fluorescence imaging 70 3 6 2 Automatic fluorescence exposure 73 3 6 3 Manual fluorescence exposure 76 3 6 4 SNOW fluorescence exposure 66 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture ...

Page 67: ...xample from a gel with Colorimetric marker it is recommend to capture images firstly with the White insert for Mw determina tion and then without the White insert for best fluorescence detection of weak bands Note The Fluorescence tab The illustration below shows the main components of the Fluorescence screen Function Part Function Part Select binning 8 Save image capture settings as a method 1 Se...

Page 68: ... IRshort 660 IRshort IRlong 775 IRlong To select which dye or dyes are being used in the sample use the following steps A maximum of three dyes can be analyzed at one time Action Step To select the dyes used under 2 Basic settings tap Select 1 68 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture 3 6 1 Fluorescence ima...

Page 69: ...imaging can be susceptible to background gradient effects For IRlong image capture position the sample in the centre with the lanes running horizontally i e parallel to the short sides of the tray This will minimize any potential gradient effect when measuring bands of a similar size in different lanes Amersham ImageQuant 800 User Manual 29383813 AA 69 3 Handle methods in ImageQuant 800 control so...

Page 70: ...ke an automatic exposure To take an automatic exposure use the following steps Action Step On the Home screen tap Fluorescence image capture 1 Under 1 Select Exposure tap Auto 2 Under Select dyes select the dye or dyes being used in the image 3 Tap Select to return to the method screen 4 If an overlay image of the sample and marker is required tap the toggle button next to Colorimetric marker 5 If...

Page 71: ...capture settings for future use tap Save method In the popup window enter the Name of the method and tap Save 8 Tap Pre capture Note To take an automatic exposure without predefining a region of interest tap Start without tapping Pre capture 9 Tap the button to toggle between Point and Area measurement 10 Amersham ImageQuant 800 User Manual 29383813 AA 71 3 Handle methods in ImageQuant 800 control...

Page 72: ...me of reference toggle to Area Tap and drag to select the desired reference area 1 Note The area can be moved without redrawing it by using the arrow buttons 2 Tap Start 12 72 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture 3 6 2 Automatic fluorescence exposure ...

Page 73: ... screen tap Fluorescence image capture 1 Under 1 Select Exposure tap Manual 2 To select the dyes used tap Select 3 In the Fluorescence dyes window tap the drop down arrow and select the dye being used 4 Under Exposure time tap the white box 5 Amersham ImageQuant 800 User Manual 29383813 AA 73 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture 3 6 3 Manual fluorescen...

Page 74: ...ther dye tap Add dye and select the new dye from the drop down menu 7 Result a new dye selection row appears Enter an Exposure time for the new dye 8 If needed add a third dye 9 If an overlay image of the sample and marker is required tap the toggle button next to Colorimetric marker 10 74 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 6 Fluor...

Page 75: ...age 41 for more information 11 To save the capture settings for future use tap Save method In the popup window enter the Name of the method and tap Save 12 Tap Start 13 Amersham ImageQuant 800 User Manual 29383813 AA 75 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture 3 6 3 Manual fluorescence exposure ...

Page 76: ...tens out The Target improvement ratio can be altered during a run or the process can be stopped and the image saved directly if this happens Note Take a SNOW exposure To capture an image using the SNOW function use the following steps Action Step On the Home screen tap Fluorescence image capture 1 Under 1 Select Exposure tap SNOW 2 If an overlay image of the sample and marker is required tap the t...

Page 77: ...of interest and the selected background area Select a flat background area that is free from bands stain speckles or dust If it is not possible to select such a back ground area for example if a post stained gel exhibits many dye speckles in the gel background it is recommended to select a smaller area or an area next to the gel Amersham ImageQuant 800 User Manual 29383813 AA 77 3 Handle methods i...

Page 78: ...ture the signal to noise improvement ratio will update To manually enter a target improvement ratio during image capture toggle off Auto stop tap the box next to Target improvement ratio and enter a new value 11 Result the manual boundary will show in the graph 78 Amersham ImageQuant 800 User Manual 29383813 AA 3 Handle methods in ImageQuant 800 control software 3 6 Fluorescence image capture 3 6 ...

Page 79: ...tains information on how to view and manage images in ImageQuant 800 control software In this chapter See page Section 80 4 1 View the image after capture 86 4 2 The Image library screen Amersham ImageQuant 800 User Manual 29383813 AA 79 4 Manage images in ImageQuant 800 control software ...

Page 80: ... to image capture screen 7 Toggle image overlays 2 Print image 8 Image information 3 Zoom in and out of image 9 Select editing tool 4 Editing tools 5 Image overlays Depending on the imaging type used the image overlays tabs will be different Display options Imaging method Toggle Marker on and off Chemiluminescence with colorimetric marker 80 Amersham ImageQuant 800 User Manual 29383813 AA 4 Manage...

Page 81: ...tware provides basic tools for editing captured images before saving them Function Part Invert Toggle on to invert the image colors 1 Crop Set image crops if multiple samples have been used Note Using Half crop and Quarter crop will separate the image into 2 or 4 image files when saved 2 Rotate image 3 Exposure time for each image type 4 Amersham ImageQuant 800 User Manual 29383813 AA 81 4 Manage ...

Page 82: ...ted it can be moved around the image using the arrows This function can be used to check if the exposure time was suitable and do a first estimate of signal levels in different positions on the image To measure the signal intensity use the following procedure Signal intensity measurements are not reliable in saturated images Saturated parts of an image are highlighted in purple Note 82 Amersham Im...

Page 83: ...the Image view screen tap Intensity 1 Tap and drag to select the area of interest 2 Read the intensity signals in the right table 3 Note If multiple images are being viewed the intensities of all dyes will be displayed Amersham ImageQuant 800 User Manual 29383813 AA 83 4 Manage images in ImageQuant 800 control software 4 1 View the image after capture ...

Page 84: ...without resizing 4 Time series images When taking a Time series exposure different options are available on the View image screen 84 Amersham ImageQuant 800 User Manual 29383813 AA 4 Manage images in ImageQuant 800 control software 4 1 View the image after capture ...

Page 85: ...y selected image on full screen 8 Select which individual image to save 3 Zoom in all images 9 Toggle colorimetric Marker on and off 4 Zoom out all images 10 Selected image for full screen in dicated by blue border 5 Amersham ImageQuant 800 User Manual 29383813 AA 85 4 Manage images in ImageQuant 800 control software 4 1 View the image after capture ...

Page 86: ...age information 7 Toggle between list and grid thumbnail view 1 Open selected image 8 Image thumbnails from select ed folder 2 Copy move image 9 Select image folder 3 Delete image 10 Select imaging method 4 Select all images 11 Sort images 5 Search images 6 86 Amersham ImageQuant 800 User Manual 29383813 AA 4 Manage images in ImageQuant 800 control software 4 2 The Image library screen ...

Page 87: ...s can be selected at once Tap Open to open the image file 4 Result the image or images are opened in the Image view screen Note If multiple images have been selected the option to Open is not available The option to Copy Move this images is available for multiple images Move image Image files can be copied and moved in Amersham ImageQuant 800 control software Action Step In the Image library scree...

Page 88: ...ol software can print images directly to a printer connected on the network To do this use the following steps Action Step In the View image screen tap Print 1 Tap to select the printer in the Print window 2 Tap Print 3 Save locations Save locations can be added and the default save location can be chosen in the Settings screen see Default save location on page 21 88 Amersham ImageQuant 800 User M...

Page 89: ... ImageQuant CONNECT software and provides information on how to use the different functions In this chapter See page Section 90 5 1 Introduction 91 5 2 Use the scheduler tool 94 5 3 Manage images Amersham ImageQuant 800 User Manual 29383813 AA 89 5 ImageQuant CONNECT operation ...

Page 90: ...ing the machine as well as view and download image files from the instrument The image below shows a schematic representation of the Amersham ImageQuant 800 system network Function Part Amersham ImageQuant 800 instrument 1 External PC connected to Amersham ImageQuant 800 2 Local network 3 Computers connected to local network 4 90 Amersham ImageQuant 800 User Manual 29383813 AA 5 ImageQuant CONNECT...

Page 91: ...Amersham ImageQuant 800 instrument remotely The Scheduler screen Function Part Function Part Scheduler tab 5 Instrument status 1 Help 6 Selected time slot 2 Create new booking 7 Refresh calendar 3 Delete booking 8 Select week 4 Amersham ImageQuant 800 User Manual 29383813 AA 91 5 ImageQuant CONNECT operation 5 2 Use the scheduler tool ...

Page 92: ...indow additional details can be added in the text box next to User and Comment 5 Enter the desired end time for the time slot by clicking in the drop down menu 6 Note By default time slots are set to 15 minutes Click Save 7 Edit a booking To edit a booking use the following steps Action Step In the Scheduler screen select the booking to be edited 1 92 Amersham ImageQuant 800 User Manual 29383813 A...

Page 93: ...n the Edit booking window enter the new information 3 Click Save 4 To delete a booking select the booking slot and click Delete Amersham ImageQuant 800 User Manual 29383813 AA 93 5 ImageQuant CONNECT operation 5 2 Use the scheduler tool ...

Page 94: ...pen image 7 Select folder 2 Copy image 8 Filter by method 3 Select all images in the folder 9 Sort images by date or alphabeti cally 4 Toggle between list and thumb nail image view 10 Search images 5 94 Amersham ImageQuant 800 User Manual 29383813 AA 5 ImageQuant CONNECT operation 5 3 Manage images ...

Page 95: ... folder will display in the image window Click on the desired image or images Note The Method Sort by and Search tools can be used to help find specific images 2 Click on the desired image to display the image information in the right panel Note If more than one image is selected no information will be displayed 3 Click Open 4 Result the image is opened in the image editing screen Amersham ImageQu...

Page 96: ...ges tab click Close 6 To save a local copy of the image click Download 7 Navigate to the local save folder in the browser and click Select folder 8 Result a new folder will be created at the selected location containing a jpg and tiff version of the file Measure the signal intensity The Intensity function can be used to measure the signal intensity of either a single point or a defined area of the...

Page 97: ...he product If the suggested actions in this guide do not solve the problem or if the problem is not covered by this guide contact your GE Healthcare representative for advice In this chapter See page Section 98 6 1 Instrument problems 101 6 2 Image problems 103 6 3 Software problems 104 6 4 Error messages Amersham ImageQuant 800 User Manual 29383813 AA 97 6 Troubleshooting ...

Page 98: ... button on the right side of the instrument 4 Wait for approximately 10 seconds and turn the system on again 5 If the error persists refer to this troubleshooting guide for more information 6 If the error cannot be resolved using this troubleshooting guide contact your GE Healthcare representative 7 General problems This section describes possible causes and corrective actions for various problems...

Page 99: ...ve A hardware error has oc curred The Indicator is illuminated red Close the door and repeat the exposure The door is open during ex posure Turn off the instrument s Power switch im mediately and contact your GE Healthcare representative The light source is lit or the motor is running when the door to the instrument is open an interlock failure has occurred Operation is not stopped when the door i...

Page 100: ...all connections between the instrument and external computer are secure The instrument is not con nected to the external computer The instrument does not appear to respond to soft ware control 100 Amersham ImageQuant 800 User Manual 29383813 AA 6 Troubleshooting 6 1 Instrument problems ...

Page 101: ...ly on the sample tray and place the tray in the correct tray position The size of the object ex posed does not coincide with the exposed area Close the door to the instrument and ex pose the image again The door to the instrument is not completely closed Light leaks on the image Move the instrument to a place that is not in direct sunlight The instrument is exposed to direct sunlight Turn off the ...

Page 102: ...vious images are visible Place a single membrane in a central posi tion in the imaging area Use local back ground correction to reduce the gradient background see Sample positioning for IRlong on page 69 Membrane is placed out side of central imaging area 80 110 cm Gradient background in IR long images of membrane samples Capture additional fluorescence images for analysis without using the White ...

Page 103: ...rk See Amersham ImageQuant 800 Operating Instructions Click sync to refresh the list of instruments Instrument connection problem Instrument is not visible in the instrument drop down menu in Amersham ImageQuant CONNECT Return to Home screen and check if instru ment is connected MakesurethattheAmershamImageQuant 800 is switched on Instrument is switched off Click sync and re enter the booking Netw...

Page 104: ...ective action Image Description The selected save location cannot be found Tap Close and select a new folder in which to save the image Alert Save location Tap OK Make sure that system doors are firmly shut and take the image again Capture was interrupted Tap OK change the IP address of the new instrument to a unique identifier IP address al ready exists 104 Amersham ImageQuant 800 User Manual 293...

Page 105: ...tray Tap OK and set a longer exposure time in the method settings Set interval time to 10 s or longer The images captured in this session have not been saved To return to the software and save the image tap No To exit the software without saving and new images or methods tap Yes Save prompt Amersham ImageQuant 800 User Manual 29383813 AA 105 6 Troubleshooting 6 4 Error messages ...

Page 106: ...87 correction files 20 editing 81 histogram 82 Image view screen 80 Measure signal intensity 82 open 87 print 88 save 21 Image editing 81 M Multi well plates adjust focus 16 imaging 49 N Network connect instrument 31 sync instruments 34 Non parallax lens imaging 49 Notes and tips 6 O Optical density measure ment 65 P Password 22 change password 26 reset password 28 Pre capture 50 70 S Signal to no...

Page 107: ...ct to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Björkgatan 30 751 84 Uppsala Sweden www gelifesciences com...

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