GE HEALTHCARE Biacore T100, Handbook

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Biacore T100 Software Handbook BR-1006-48 Edition AE
93
Methods 5
information that is relevant to the assay but not required by an
application-specific evaluation procedure (an example might be the
sample batch number). Evaluation variables may only be defined for
Sample and General commands.
Variables are set in the list at the right in the command panel. For method
variables, check a parameter to set it as variable.
Sample solution is checked by
default for Sample commands. For evaluation variables, choose the purpose of
the assay to display an appropriate list of predefined variables and check the
variables you want to use. Click
Add to set up user-defined variables.
Note: For specific assay purposes, you should generally check all suggested
variables. If you leave some variables unchecked, the assay-specific
evaluation may not work.
Predefined evaluation variables for different assay purposes are described in
the table below (see also Section 5.10).
Evaluation purpose: General, Kinetics/Affinity, Thermodynamics
Conc Analyte concentration. Multi-cycle kinetics requires a
single concentration variable. Single-cycle kinetics
requires one concentration variable for each sample
injection in the cycle.
MW Analyte molecular weight: required for molecular
weight adjustment of report points, and for kinetic
evaluation when the concentration is entered in
weight-based units.