GE HEALTHCARE Biacore T100, Handbook

Page: 36 / 238

4 Application wizards
4.2 Common wizard components
36
Biacore T100 Software Handbook BR-1006-48 Edition AE
Parameter limits
Flow rates can be set between 1 and 100 µl/min in increments of 1 µl/min.
Permitted ranges for injection contact times are determined by the flow rate
together with the limits for injected volumes, which are 2–350 µl for normal
solutions and 5-100 µl for viscous regeneration solutions (see below).
Note: The injected volume of solution is determined by the combination of flow
rate and contact time, rounded to the nearest whole number. At low flow
rates, this can result in actual contact times that differ from the requested
times: for example, at 1 µl/min a requested contact time of 200 s
(requiring 3.3 µl solution) will result in an actual contact time of 180 s
(solution volume rounded to 3 µl).
Stabilization time after injection
This function is available after a capture injection and after the last injection in
the sequence. For capture injections, a stabilization time can be useful if a
fraction of the ligand dissociates rapidly. Including a stabilization time to allow
for such dissociation can help to improve reproducibility.
A stabilization time may be used after the last injection instead of regeneration
for systems where analyte dissociates completely from the surface.
Exposure of the surface to regeneration solution can often lead to transient
changes in the baseline. Inclusion of a stabilization time after regeneration
helps to ensure a stable baseline for the next cycle.
Sample injection
Normally, the injected sample solution is specified in a separate sample table.
Some wizards (e.g.
Surface Performance ) use only a single sample solution that
is specified together with the other injection parameters in this dialog box.
Regeneration
The parameters for regeneration include a check-box for High viscosity
solution . Check this box if the regeneration solution has a relative viscosity
higher than about 3 (corresponding to about 35% glycerol or 40% ethylene
glycol at 20°C). This will modify the injection procedure for better handling of
viscous solutions. The maximum injected volume is limited to 100 µl for viscous
solutions.
4.2.4 Sample and control sample tables
Details of samples and control samples (where applicable) are entered in the
Sample and Control Samples steps respectively. The details of these steps
differs according to the wizard purpose, but the following general features may
be noted. Further details are given in the respective wizard descriptions later in
this chapter.