background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

Copyright 

©

 2009 Flexcell International 

FLEXCELL, BioFlex, FlexLink and FlexSoft are trademarks of Flexcell  International Corporation.  MS Windows and MS Office are trademarks 
of the Microsoft Corporation.  Adobe Acrobat is a trademark of Adobe Systems Inc. Dell OptiPlex is a trademark of Dell. 
General Notice: Other product names mentioned in this manual are for identification purposes only and may be trademarks of their respective 
owners.  

Flexcell International 

disclaims any and all rights in those marks. 

1.  FLEXCELL

®

 FLEX JR.

 TENSION SYSTEM COMPONENTS 

 
1. 

 

Flex Jr.

 Tension FlexLink

 

2. 

 

Laptop 

computer 

with 

preinstalled 

software: 

 

FlexSoft

®

 

Flex Jr.

™ 

V2.0 

Microsoft Windows

 10 

Do not delete any software or change any 
settings on this computer. It has been pre-
configured  to  operate  the  FlexSoft

®

  Flex 

Jr.

 software. 

3. 

 

FlexLink

 

power cable 

4. 

 

Ethernet cable 

5. 

 

Flexcell

  Single  Plate  BioFlex

®

  vacuum 

baseplate 

6. 

 

Gasket 

7. 

 

25mm Loading Station 

8. 

 

BioFlex

®

 Cells Seeder Loading Station 

9. 

 

Silicone lubricant  

10. System drying filter 
11. 20  ft.  small  blue  tubing  (

¼”  (6.4  mm) 

O.D.)

 for 

FLEX IN

 connection 

12. 20  ft.  clear  blue  tubing  (⅜”  (9.5  mm) 

O.D.) for 

FLEX OUT

 connection. 

13. 20  ft.  clear  green  tubing  (

⅜”  (9.5  mm) 

O.D.)

  for 

SYSTEM

  (vacuum  source) 

connection 

14. User manual and other ancillary manuals 

for computer and monitor 

15. Surge protected outlet strip 
16. Water Trap 
17. Vacuum source (optional) 

 

2. SETUP OF THE FLEXCELL

®

 FLEX JR.

 TENSION SYSTEM 

 

Do  not  power  up  the  Flexcell

®

  Flex  Jr.

 

Tension system until all connections among 
components have been properly made and 
checked! Never power up the computer or 
the  Flex  Jr.

  Tension  FlexLink

®

  with  the 

cover removed - an electrical shock hazard 
exists.  Operation  of  the  computer  without 
the cover is in violation of FCC regulations 
and will void the warranty!   

 

2.1  P

ACKING 

L

IST

 

 
Remove  the  packing  list  and  check  off  the 
instrument  components  as  they  are  removed 
from the shipping containers. If any parts are 
missing, contact Flexcell

®

 International Corp. 

immediately. 
 
 
 
 

2.2  L

OCATION OF THE 

F

LEX 

J

R

.

 

T

ENSION 

S

YSTEM

 

 
Choose a location for the Flex Jr.

 Tension 

system (Fig. 1) that is adjacent and as close 
as  possible  to  the  CO

2

  incubator  (or 

microscope)  and  vacuum  source.  A  shorter 
distance  in  vacuum  lines  decreases  the  air 
flow resistance for elongation and release of 
the flexible bottomed culture plate, allowing 
for  a  greater  range  of  flexing  at  higher 
frequencies.  All  tubing  and  the  Ethernet 
communications cable connect to the rear of 
the 

Flex  Jr.

  Tension  FlexLink

®

  (Fig.  2). 

The length of the tubing from the FlexLink

®

 

to  the  baseplate  (or  microscope)  device 
should  be  10'  (3  m)  long  to  assure  proper 
system performance. This length can vary if 
needed, but no more than 5' (1.5 m) longer 
or shorter. Cut the tubing 

after

 the system is 

fully set up to ensure of the proper length. 

Summary of Contents for Flex Jr.

Page 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Page 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Page 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Page 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Page 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Page 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Page 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Page 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Page 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Page 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Page 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Page 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Page 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Page 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Page 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Page 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Page 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Page 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Page 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Page 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Page 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Page 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Page 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Page 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Page 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Page 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Page 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Page 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Page 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Page 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Page 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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