background image

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

17 

experiment and when the system will not 
be operated again for more than one day. 
See section 3.16 for instructions. 

 
E. 

 

Achieving 

the 

highest 

possible 

combinations  of  %  elongation  and 
frequency: 

 

a)

 

Shorten  the 

FLEX  IN

FLEX  OUT

and 

SYSTEM

 

tubing 

to 

their 

minimum 

possible 

lengths. 

See 

section 2.2 for minimum lengths. 

 
b)

 

Use a more powerful vacuum source if 
recommended  specs  are  not  being 
reached  with  the  current  vacuum 
source (see section 2.4). 

 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

5. SYSTEM TROUBLESHOOTING 

 

NOTE:  If the Flex Jr.

 system is not functioning 

properly and the solution is not clearly outlined or 
defined  below,  please  contact  Flexcell

®

  before 

attempting any repairs.  

 

Problem 1: Regimen wave is flatlining 

 

Solution 1:   

Remove  the 

SYSTEM

  vacuum  line  at  the 

back  of  the  FlexLink

®

  and  check  to  be  sure 

that there is vacuum coming through the line. 
Check  the 

VENT

  port  at  the  back  of  the 

FlexLink

®

 when the system is running to see 

if air is moving in or out. If not, it is possible 
that  the  valves  are  locked  due  to  residue 
buildup. Try applying positive pressure to the 

VENT

 port to free the valves.  

 
If the regimen wave is flatlining but the plates 
are still cycling air pressure, try the following: 
 
Disconnect  the  blue  1/4"  (6.4mm) 

FLEX  IN

 

tubing from the back of  the unit and place  a 

finger  over  the  end  of  the  tubing  while  a 
regimen  is  running.  If  air  is  cycling  at  the 
end  of  the  tubing  then  the  problem  is  with 
the  Flex  Jr.

  Tension  FlexLink

®

.  Contact 

Flexcell

®

 

International 

for 

further 

instructions.  
 

Problem 2: Regimen wave is fluttering or 

not smooth 

 

Solution 2: 

This  problem  could  be  due  to  water  in 

the valves or miscalibration. Run the drying 
regimen with the drying filter (as described 
in the manual) to dry the system and valves. 
If  this  does  not  resolve  the  problem  try 
running the drying regimen once more. If the 
problem  continues,  contact  Flexcell

®

  for 

further instructions. 
 

Summary of Contents for Flex Jr.

Page 1: ...sion 13 1 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexce...

Page 2: ...k 5 2 8 Using Additional Flex Jr Tension FlexLinks 5 3 Operation Instructions 6 3 1 Starting the Flex Jr Program 6 3 2 Using the Flex Jr Tutorial 6 3 3 Main Display Window 7 3 4 Configuring Users 8 3...

Page 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Page 4: ...ument case Doing so will void the warranty provided through Flexcell International Corporation The Acer Aspire Laptop that is provided with the Flexcell Dynamic Culture System Flex Jr Tension system c...

Page 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Page 6: ...ngth of 9 5 mm tubing to the baseplate labeled BPLT side of the water trap c Cut the tubing to a length that will reach the baseplate in the incubator and attach the provided quick disconnect to the o...

Page 7: ...The plate should begin flexing Make a visual check to verify membrane movement 19 To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop when the regimen is c...

Page 8: ...computer and monitor 15 Surge protected outlet strip 16 Water Trap 17 Vacuum source optional 2 SETUP OF THE FLEXCELL FLEX JR TENSION SYSTEM Do not power up the Flexcell Flex Jr Tension system until al...

Page 9: ...of the system is 16 4 lbs 7 44 kg Figure 3 A Acer Aspire laptop right side view B Acer Aspire laptop left side view C Connected Ethernet cable 2 3 SETUP OF THE COMPUTER AND THE FLEX JR TENSION FLEXLIN...

Page 10: ...d securing clips Connect the system tubing as follows Fig 1 4 1 Connect the 9 5 mm O D green tubing from the pressure reservoir or vacuum source to the SYSTEM port on the back of the FlexLink 2 Attach...

Page 11: ...lex Jr tubing adapter 2 5 CONNECTING THE SYSTEM VACUUM LINES Connect the blue 9 5 mm vacuum inlet tubing to a vacuum source A vacuum pump capable of producing a maximum vacuum of 100 kPa and a flow ra...

Page 12: ...downloaded again It is recommended that the monitor be put in sleep mode when not in use and that the computer be allowed to run at full power during an experiment 2 7 LCD DISPLAY ON THE FLEX JR TENS...

Page 13: ...Jr System including limits of maximum elongation in the silicone elastomer membrane rebound of the membrane friction and frequency all waveforms cannot be reproduced accurately at every frequency and...

Page 14: ...beled Smoothing One expects data patterns to represent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should...

Page 15: ...the FlexLink during regimen activity When using a platform configuration that uses vacuum to generate a downward stretching of the membrane elongation the vertical scale represents increasing elongati...

Page 16: ...sequence of steps below 1 Type a regimen name in the box labeled Regimen Name 2 Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop do...

Page 17: ...ick on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deleti...

Page 18: ...erall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be M...

Page 19: ...s the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g risi...

Page 20: ...form the user the regimen name and a sequence number If a tension regimen is run three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 respective...

Page 21: ...ta 3 16 SHUTTING DOWN THE SYSTEM A system drying filter Fig 13 has been supplied with the Flex Jr This device must be used whenever the system has been operated for more than one day and will not be o...

Page 22: ...n the drying filter turn pink the filter is saturated and the beads needs to be regenerated See Tech Report 200 Use of Water Trap and Drying Filter with the Flexcell Tension Systems for instructions o...

Page 23: ...hen the Water Trap becomes half full On the first trial monitor the Water Trap every 15 minutes for the first two hours to be sure that a significant amount of water is not accumulating If so there ma...

Page 24: ...he line Check the VENT port at the back of the FlexLink when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to residue buildup Try applyin...

Page 25: ...ate See the solution to Problem 1 to correct this Be sure to empty the Water Trap frequently if this problem occurs Water accumulation in the FlexLink can damage the valves Problem 6 Cells are not att...

Page 26: ...x with a quick transition at the min Sine Wave This waveform provides a smooth physiological variation in pressure from min to max Triangle Wave This waveform provides a linear ramped rise to the max...

Page 27: ...ongation Applies a defined controlled static or cyclic deformation to cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified strain regimen Cap...

Page 28: ...tion User manual Vacuum Source Connection User Manual Water Trap and Drying Filter Tech Report and other ancillary manuals for computer Surge protected power outlet strip FLEX JR TENSION COMPUTER SPEC...

Page 29: ...1 9 Maximum achievable elongation with the Flex Jr 13 4 StageFlexer 31mm Diameter Loading Stations equibiaxial strain Minimum achievable elongation with the Flex Jr 2 1 Maximum achievable elongation...

Page 30: ...n off the baseplate see 6 Application Notes 4 Open the water trap drain to remove any accumulated moisture and then close the water trap drain 5 Connect drying filter and run the 4 hour drying regimen...

Page 31: ...ect gmail com Australia New Zealand Cell Systems Biology enquiries cellsystemsbiology com www cellsystemsbiology com Taiwan Nature Opera Biotechnology Inc sales naturebiotech com tw www naturebiotech...

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