Fisher Scientific accuSpin 3 Operating Instructions Manual Download

Page: 3 / 82

speed/RCF

Quick run

run time

open lid

stop

start

The

mains switch

is located on the right-hand

side panel.
Pressed up = ON

Pressed down = OFF 0

(only for instruments with refrigeration unit)

temperature

program selection

Bucket selection

rpm/RCF

switch key

display

"set" keys

pre-temperature-regulation

Before switching on the centrifuge
please read this manual

deceleration
profiles

acceleration
profiles

Summary of Contents for accuSpin 3

Page 1: ...accuSpin 3 3R OPERATING INSTRUCTIONS...

Page 2: ...nnot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessorie...

Page 3: ...l Pressed up ON I Pressed down OFF 0 only for instruments with refrigeration unit temperature program selection Bucket selection rpm RCF switch key display set keys set keys pre temperature regulation...

Page 4: ...ature in C in temperature equilibrium only for instruments with refrigeration unit Keys start normal start of the centrifuge stop manual stop of a run open lid open lid possible only with the instrume...

Page 5: ...t operation 25 Checking of aerosol tight bio containment 27 Operation 29 Switching on the centrifuge 29 Actuating the lid 29 Opening the lid 29 Closing the lid 29 Installing the rotor 30 Loading the r...

Page 6: ...trifuging with a program 42 Quick Run 43 Removing the rotor 43 Audible alarm 44 Turning the centrifuge off 44 Maintenance and care 45 Maintenance to be performed by the customer 45 Cleaning 45 Disinfe...

Page 7: ...imes When damages at the power cord or at casing were noticed the centrifuge has to be set out of operation Proper use The centrifuge is designed to separate liquid suspended materials having differen...

Page 8: ...riginal accessories for the centrifuge The only exception are common glass or plastic centri fuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the roto...

Page 9: ...a tions for all voltages IEC 61010 for 120 V only for 230 V only Details of the test standards take you please from the technical data Safety instructions in this manual This symbol denotes potential...

Page 10: ...For your safety 6 Notes...

Page 11: ...s more rapidly than competitive instruments and can process nominally three liters of sample in a single run There are various rotors with high RCF values that can ac commodate a wide range of accesso...

Page 12: ...and the rotor has come to a stop You can start the centrifuge only if the lid is properly locked Automatic rotor identification Electronic imbalance detection as a function of rotor SMARTspinTM Do no...

Page 13: ...riven by Easycontrol II Main memory Recalls last run parameters Program memory data are stored until new values are entered Advanced features RCF programming quick run pretemp temperature control duri...

Page 14: ...increments RCF selection upon activation of RCF switch the RCF value can then be entered Time selection adjustable in minutes from 1 min to 9 h 59 min or extended time mode from 1 min to 99 h hld mode...

Page 15: ...ivates maximum acceleration up to the maximum permissible speed of rotor upon key release centrifuge stops with maximum deceleration power Diagnostic messages alternating display Rotor maximum speed o...

Page 16: ...Description of the accuSpin 12 Notes...

Page 17: ...and secure it properly Handle the centrifuge carefully Proper location The centrifuge may only be used indoors Its location must meet the following criteria A safety zone of at least 30 cm 12 inches...

Page 18: ...n connection Connect the centrifuge only to an grounded main power supply Make sure that the power cord is com patible with the safety regulations valid and that your main voltage and frequency corres...

Page 19: ...s part of a accuSpin 3 or 3R centrifuge In addition there are adapters and reduction sleeves for a variety of commercially available tubes and bottles Please consult our sales documentation for a com...

Page 20: ...Rotors and accessories 16...

Page 21: ...ad g 4 x 800 4 x 750 4 x 500 Maximum speed nmax rpm 4150 4350 3750 4350 4150 4350 Maximum RCF value at nmax 3 696 4083 2 986 4020 3061 3364 Radius max cm 19 2 19 0 15 9 Acceleration deceleration time...

Page 22: ...4 x 800 4 x 750 4 x 500 Maximum speed nmax rpm 4150 4600 3750 4600 4150 4600 Maximum RCF value at nmax 3696 4566 2986 4494 3061 3761 Radius max cm 19 2 19 0 15 9 Acceleration deceleration time s 60 35...

Page 23: ...ml 7500 3337 Maximum permissible load g 24 x 4 Maximum speed nmax rpm 15 000 Maximum RCF value at nmax 21 885 Radius max min cm 8 7 5 9 Tube angle 45 Acceleration deceleration time s 30 35 Min temper...

Page 24: ...wn 7500 8137 12 x 14 15 ml with flange 18 3 x 125 21 5 48 brown 7500 6494 7 x 25 ml DIN 25 0 x 125 31 0 28 orange 7500 8138 7 x 45 50 ml flat and round bottom 29 5 x 125 31 0 28 blue 7500 6493 4 x 50...

Page 25: ...14 15 ml with flange 18 3 x 117 25 0 36 brown 7500 6492 6 x 25 ml DIN 25 0 x 117 28 0 24 orange 7500 6457 4 x 25 50 ml universal container 25 5 x 117 32 0 16 blue green 7500 6459 4 x 45 50 ml flat and...

Page 26: ...iter Rotor 7500 3337 max tube dimensions d 1 x length mm tube capacity ml number per set color order no Reduction sleeve PCR 6 2 x 20 0 2 24 grey 7600 3750 Reduction sleeve 8 x 43 5 0 5 0 6 24 turquoi...

Page 27: ...ing bucket rotors at regular inter vals apply a light coating of lubricant to the rotor body trunnion pins and to the corre sponding mating surfaces on the buckets Lubricant 7000 6692 is supplied with...

Page 28: ...before loading that the rubber bottom is placed in the cut outs of the bottom of plate carrier Deepwell plates can also be inserted in the carrier without using plate holder Insert the individual pla...

Page 29: ...equisites for aerosol bio containment Before use the seals in the rotors and rotor covers as well as the aerosol tight caps have to be checked for abrasion or damage and slightly greased Replace damag...

Page 30: ...n the cover clockwise by 1 grip area approx 15 Use the setting marks on the bucket as a guide Micro Liter Rotor 24 x 2 0 ml Attention Please check that your sample containers are suitable for the cent...

Page 31: ...ng tested for wear and damages like cracks scratches and embrit tlement carefully As a quick test there is the possibility to check the aerosol tight buckets and fixed angle rotors according to the fo...

Page 32: ...Rotors and accessories 28 Notes...

Page 33: ...ning run time and speed should both read 0 The display of the acceleration deceleration curve depends on the last set value The following figure gives an example of possible read ings A detailed descr...

Page 34: ...socket wrench supplied 3 Place the rotor on top of the collet chuck so that the rotor is located precisely above the center 4 The rotor must glide freely down the collet chuck until it hits the lower...

Page 35: ...to centrifuge samples that together with the adapters exceed the maximum permissible load you must either reduce the sample volume or calculate the permissible speed nperm according to the following f...

Page 36: ...ging bucket rotor 7500 4393 this amounts to at least 20 g in opposite carriers The values refer to a weight difference on the bottom of the tube If there is a weight difference resulting from inhomoge...

Page 37: ...Operation 33 Swinging bucket rotors These examples are to be applied to the other rotors in an analogous manner Proper loading Improper loading...

Page 38: ...Operation 34...

Page 39: ...ed display is set Use the speed mode selection key to switch speed entry and display between rpm and RCF Bucket selection for swinging bucket rotors When running a swinging bucket rotor the automatic...

Page 40: ...lease the key as soon as you have reached the desired value and adjust if necessary by repeatedly pressing the key The decimal place flashes for a number of seconds then changes to permanent display T...

Page 41: ...eases depending on the tubes and adapters used You may take this into account when calculating the RCF value for your application Selecting run time There are two time modes standard and extended In t...

Page 42: ...n to the continuous mode you must press the key until the display reads hLd With this setting the centri fuge keeps run until stopped manually Extended time mode You can optionally switch to the exten...

Page 43: ...elease the key as soon as you have reached the desired value and adjust if necessary by repeatedly pressing the key The temperature display flashes for a number of sec onds then changes to the current...

Page 44: ...inserted rotor Within 15 seconds you may adopt this value by again pressing the start key the centrifugation is then con tinued Otherwise the centrifuge stops and you must enter a permissible value Yo...

Page 45: ...tinuous operation you must stop the centrifuge manually Press the Stop key in the control panel The centrifuge starts deceleration with the preset deceleration profile The display reads End and you ca...

Page 46: ...ing After entering all set point values the desired pro gram selection key has to be pressed for approx 3 seconds the new program is being stored Repeat the process to set values to additional program...

Page 47: ...uring acceleration the time is counted forward in sec onds The display remains until the centrifuge lid is opened Removing the rotor 1 Open the centrifuge lid 2 Remove the rotor cover 3 Unscrew the cl...

Page 48: ...or off By actuating the upward key in the time panel the signal function can be switched on or off When the message rotor flashes pressing the start key once is sufficient to turn off the warning sig...

Page 49: ...ke sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor cham...

Page 50: ...please disconnect the centrifuge from the mains supply Please pull mains plug Disinfection If a centrifuge tube containing infectious material leaks during a run you have to disinfect the centrifuge...

Page 51: ...mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the centrifuge tubes and adapters and disinfect them or dispose of them...

Page 52: ...y autoclave the rotor and the adapters at 121 C Maximum permissible autoclaving cycle 20 min at 121 C The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor...

Page 53: ...Without power the brake does not function and deceleration takes much longer than normal Proceed as follows 1 Make sure that the rotor is at a stand still observe through window in the cover During a...

Page 54: ...ord back into the instru ment and reinserting the plastic plug Once the power is restored you can connect the in strument to the main supply and turn it on Following the self test of the centrifuge th...

Page 55: ...the main connection is OK contact service Displays fail briefly The drive stops suddenly The rotor stops without deceleration Brief interruption of main supply 1 Turn off main switch 2 Check whether t...

Page 56: ...or or motor malfunction is responsible for the noise If you cannot locate and solve the problem yourself contact service Message bAl appears in display Rotor stops with deceleration Imbalance switch a...

Page 57: ...ion you must wait until the rotor has stopped By opening and closing the lid you reset the message rotor After entering a permissible speed you can press start Display OPEN appears although lid is clo...

Page 58: ...r persists contact service E 15 E 16 Rotor stops with deceleration to standstill Instrument cannot be operated Temperature measurement error Switch the instrument off and on again If the problem persi...

Page 59: ...be permitted for the operation in the respective rotor Please note the hints of chapter Bucket selection of swinging bucket rotors page 35 and compare the permitted rotors and rotor buckets in table 1...

Page 60: ...using the main switch E 22 E 23 Rotor stops without brake to standstill Instrument cannot be operated Error in speed measurement Switch the instrument off and on again If the error persists contact se...

Page 61: ...r stops without brake to standstill or does not start Overtemperature in the motor 1 Turn instrument off and unplug mains power cord 2 Check and clean ventilation slots if necessary 3 After about 60 m...

Page 62: ...e intermediate circuit Switch the instrument off and on again If the error persists contact service E 35 Rotor stops without brake to standstill or does not start Overcurrent in the intermediate circu...

Page 63: ...to standstill Instrument cannot be operated Acceleration of rotor too slow Open the lid and check the load Significant imbalance caused by missing bucket Tubes or adapter do not fit rotor is rubbing...

Page 64: ...ch the instrument off 2 Keep selection key pressed and switch on the instrument For about 1 sec all displays read Subsequently the following readings will be dis played for 5 seconds each numbers are...

Page 65: ...6 F to 95 F run time standard mode 1min 9 h 59 min hold permanent extended mode 1min 99 h hold maximum speed nmax 15 000 rpm rotor dependent adjustable in steps of 10 minimum speed nmin 300 rpm maximu...

Page 66: ...R 89 kg 196 lb 131 kg 288 lb Testing standards all devices manufactured and examined in agreement also for 120 V only for 230 V only IEC 61010 1 1990 amendment 1 1992 amendment 2 1995 IEC 61010 2 020...

Page 67: ...nstrument safety fuse thermic excess current release Fuse protection of building accuSpin 3 7500 4391 120 V 60 Hz 10 2 A 900 W 15 A 15 AT accuSpin 3 7500 4386 230 V 50 60 Hz 8 6 A 1250 W 10 A 16 AT ac...

Page 68: ...64 Notes...

Page 69: ...65 Appendix Acceleration and deceleration profiles On the following pages you find acceleration and deceleration profiles for each rotor type respectively...

Page 70: ...66 Acceleration profiles Swinging bucket rotor with rectangular buckets 7500 4395 time s speed rpm 9 1...

Page 71: ...67 speed rpm time s 1 9 Deceleration profiles Swinging bucket rotor with rectangular buckets 7500 4395...

Page 72: ...68 Acceleration profiles Micro liter Rotor 7500 3337 speed rpm time s...

Page 73: ...69 Deceleration profiles Micro liter Rotor 7500 3337 speed rpm time s 9 1...

Page 74: ...s nmax max speed Speed RCF diagram Swinging Bucket Rotor 7500 4393 10 100 1000 10000 100 1000 10000 RCF x g speed rpm Micro plate swing nmax 4150 rpm RCFmax 3061 Round bucket Rectangular bucket nmax 4...

Page 75: ...71 1 10 100 1000 10000 100000 100 1000 10000 100000 rmax 8 7 cm rmin 5 9 cm nmax 15 000 rpm RCF rmax nmax 21 885 Speed RCF diagram Micro liter Rotor 7500 3337 RCF x g speed rpm...

Page 76: ...72...

Page 77: ...ng cycle permissible maximum 48 B bAL 40 Basic unit function 9 C centrifuge exceptionally noisy 52 Centrifuge transport and installation 13 Centrifuging with a program 42 changing settings during run...

Page 78: ...t control panel 7 emergency lid release 49 Entering parameters 35 entering changing a program 42 error codes Lid 53 OPEN with lid closed 53 rotor 53 error codes bAl 52 E 01 E 40 54 F filling the centr...

Page 79: ...M maintenance 45 manual lid unlocking 49 manual stopping 41 max tube dimensions 20 Maximum loading 31 maximum permissible RCF 40 maximum permissible speed 40 Maximum RCF value 17 maximum sample densit...

Page 80: ...lay 35 RCF value 18 19 readings of control panel during run 7 relative centrifugal force 37 remain dark 51 rotor cover 30 rotor identification 8 rotor identification 36 and RCF display 36 Rotor progra...

Page 81: ...41 with continuous operation 41 Stopping with preset run time 41 substructure 13 Switching from speed to RCF display and vice versa 35 symbols for hazards and dangers 5 T Technical Data 61 test aeros...

Page 82: ...inuous product development we reserve the right to make changes without express notice 2003 Fisher Scientific All Rights Reserved Printed in Germany 20057884 accuSpin 3 3R _US Rev 03 04 For Ordering o...

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