Endress+Hauser Analytik Jena CyBio Well vario, User Manual

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42 Rev B 2022/03
Technical Description CyBio Well vario
Fig. 30: Removing the residual liquid from the pipette tip
1 Residual liquid in pipette tip or capillary
2 Removal of residual liquid by gently pressing the pipette tips onto the fleece of the dabbing station
during residual ejection
3 Resetting the pistons to the zero position
4.4.2 Functionality
Thedabbing fleece is made of glass fibers and is therefore chemically inert to the greatest
possible extent.
Even when wet, the fleece still has sufficient capillarity to "passively" absorb small amounts
of liquid. The fleece should always be used in two layers. On the one hand, this increases
its absorption capacity for liquids, and on the other hand, it makes the dabbing surface
thicker and thus softer. This better ensures that all tips touch down on the fleece and that
the residual liquid thus comes into close contact with the fleece.
This is also supported by the fact that the surface of the dabbing station is mounted on
springs. The fleece itself is placed on a porous glass plate. By means of a vacuum, liquid is
extracted from the fleece and transferred to a waste container. This does not dry the
fleece!
After residual ejection of reagents or test substances on the dabbing station, cross-con-
tamination could also occur when dabbing again. Therefore, it is necessary to clean the
fleece and, at the same time, the tips or capillaries after such a transfer. This is done by
taking up the washing liquid into the tips and discharging it just above the surface of the
fleece, followed by a residual ejection with contact to the fleece and parallel vacuum aspi-
ration. This cleans both the tips and the fleece (
→
Fig. 31 ).