Biometra TRIO
Protocol Wizard
Version 03_19
67
ometra TRIO protocol wizard parameter screen (see Figure 43) to access the primer
annealing temperature calculator screen (see Figure 44).
C
ALC
T
A
In the annealing temperature calculator screen enter the sequences for the forward
and reverse primer using the button below the input fields. The sequence length is
counted by the software and displayed in brackets left to the input fields.
Figure 46 Biometra TRIO primer annealing temperature calculator screen
To adjust the primer or salt concentration press:
E
DIT CONCENTRATION
In the next screen the primer concentration, salt concentration and magnesium con-
centration can be adjusted. Touch the corresponding input field and enter values using
the keypad. The concentrations have the following default values and ranges:
Function
Default value
Range
Primer concentration
200 nM
0-2.000 nM
Salt concentration
50 mM
0-200 mM
Magnesium concentration
0 mM
0-200 mM