Endress+Hauser 30-5015-100-24 Operating Manual Download

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Operating Manual

CyBio FeliX

Summary of Contents for 30-5015-100-24

Page 1: ...Operating Manual CyBio FeliX...

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Page 3: ...n may be duplicated photocopied saved to a storage system or transferred to electronic media without prior written permission of the publisher Publisher Analytik Jena AG Konrad Zuse Strasse 1 07745 Je...

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Page 5: ...used EN ISO 12100 Sicherheit von Maschinen Allgemeine Gestaltungsleits tze Risikobeurteilung und Risikominderung Safety of Machinery General principles for design Risk assessment and risk reduction EN...

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Page 9: ...n auf begr ndetes Verlangen die speziellen Unterlagen zu dem unvollst ndigen Produkt ber unsere Dokumentationsabteilung zu bermitteln Das unvollst ndige Produkt darf erst in Betrieb genommen werden we...

Page 10: ...X 2018...

Page 11: ...2 Standards Guidelines 12 3 3 Safety Labeling Provided at the System 13 3 4 Mechanical Danger Zones 17 3 5 Protective Devices 18 3 6 Requirements for Operating Personnel 18 3 7 Safety Requirements fo...

Page 12: ...equirements 43 6 1 1 Installation Requirements 43 6 1 2 Footprint Requirements 43 6 1 3 Energy Supplies 44 6 2 Initial Start Up Configuration 45 6 3 Function Tests 46 6 3 1 Precision Test 46 6 3 2 Acc...

Page 13: ...s 76 8 4 1 Replacement of Inlet Fuse s 76 8 4 2 LED Blinks red 77 8 4 3 Restoring Readiness to Operate After Break Events Expl Power Failure 77 8 4 4 Manual axis movement 77 9 Maintenance Care 79 9 1...

Page 14: ...XIV 2018 CyBio FeliX 11 5 Further Accessories 96 11 6 Spare Parts 96 12 Waste Disposal 97 12 1 Consumables 97 12 2 Reagents 97 12 3 System Accessories 97...

Page 15: ...1 Fig 23 Removal of pipetting head 51 Fig 24 Head mount with clamping lever in front position 51 Fig 25 Directions of move and coordinate pattern view 52 Fig 26 Commands 58 Fig 27 Settings Set tool in...

Page 16: ...XVI 2018 Fig 1 Clean bench and door monitoring set 102 Fig 2 Door monitoring set 103 Fig 3 Clean bench 104 Fig 4 Safety light curtain 104 Fig 5 Pin assignment for connecting ESTOP IN 107...

Page 17: ...d take appropriate measures for fault removal This User Manual must be readily available to operating and maintenance personnel at all times 1 2 Target Group This User Manual addresses Qualified and p...

Page 18: ...to the table below Table 1 Graphic design styles of cross references Type of cross reference Graphic style Explanatory note Reference to an illustration Fig 1 Illustration is on the same page Referenc...

Page 19: ...May result in death or serious injury if not avoided CAUTION Dangerous situation Potential consequences light or moderate physical injury NOTICE Dangerous situation Potential consequences material da...

Page 20: ...rch Its basic functions are the aspiration and dispensing of liquid from and into a microplate reservoir column single well or tube The term conforming use also presupposes that the system is operated...

Page 21: ...mental protection and waste disposal The user is advised to issue special operating instructions where the system is to be operated with the involvement of hazardous substances Accordingly this User M...

Page 22: ...damage In the event of personal injury or material damage no claim for warranty and liability will be accepted unless the system is operated as specified in section Conforming Use on page 4 1 7 Scope...

Page 23: ...h approx 650 x 665 7001 x 450 mm 1 From the Serial Number SN 305015 10 1001 Weight Basic unit 30 5015 100 24 Pipettor in operational state approx 50 kg approx 58 kg Specifications regarding selectable...

Page 24: ...650 x 6451 6652 x 450 mm 1 Model 30 5015 500 24 2 Model 30 5015 401 24 Weight 30 5015 500 24 30 5015 401 24 approx 39 kg approx 50 kg Specifications regarding selectable microplates Formats SBS stand...

Page 25: ...acity Fuse Buerklin 42 G 1786 Power consumption 2 A Interfacing points Sub D 9 pole jack RS 232 IN Sub D 9 pole plug RS 232 router USB jack type B RJ 45 Ethernet Airborne sound emission 70 db A Operat...

Page 26: ...10 2018 CyBio FeliX Technical Specifications...

Page 27: ...a defective state or with safety and protection devices installed in a nonconforming manner Operate the system only at a line voltage that complies with the label specifications Observe prescribed ma...

Page 28: ...appropriate an until the EC declaration of conformity according to annex II A is issued 2 All specifications relating to safety reference the European Union regulations in their latest binding revisi...

Page 29: ...or its components and must be strictly observed Before switching on the device check that warning notes and safety symbols are com plete and intact Do not put the device into operation if warning not...

Page 30: ...ions Pull the mains plug Wear hand protection Define type and quality of the protection mea sure3 within the frame work of the workplace evaluation 3 If necessary refer to EN ISO 374 Protective gloves...

Page 31: ...ing China RoHS label The device contains substances subject to regulation according to the directive Man agement Methods for the Restriction of the Use of Hazardous Substances in Electrical and Electr...

Page 32: ...16 2018 CyBio FeliX Safety Notes Fig 2 Warning signs at the system Fig 3 Warning sign at the pipetting head...

Page 33: ...ve motorized positional shifts in X and Z 2 Deck move range motorized positional shifts in Y Follow these general rules Do not place your hands or fingers including objects you are holding into a mech...

Page 34: ...riate personal protective equipment should be applied if necessary Before a break in operation or on completion of work adequate measures should be taken for skin cleaning and skin protection 3 7 Safe...

Page 35: ...re inside of the operating room Operating personnel must be duly informed about the locations and the proper handling of fire extinguishing equipment in the operating room 3 8 3 Electrical Work on ele...

Page 36: ...on measures is shown in the chapter Safety Labeling Provided at the System on page 13 The operator of the device is the sole responsible for ensuring compliance with all safety requirements to protect...

Page 37: ...ompatible with the materials listed The following components have direct contact with the processed substances Table 7 Components Component Material Pipette tips PP Piston seals1 1 Aerosols may lead t...

Page 38: ...ine Acetone Cleaning spray Ozone Oxidative solutions Sodium hypochlorite Halogens Highly concentrated alkaline solutions Disinfection Method Disinfectant1 1 Depending on the application it may be poss...

Page 39: ...QUA MAGNUM FLX Enhanced Universal Magnet Plate OL3317 11 285 Waste box I small 844 00430 0 Tip transfer tool 96 250 l DW filter 96 1000 l OL3396 352 25 OL3396 25 354 TipRack 96 1000 l OL3317 11 140 96...

Page 40: ...personnel must be aware of the locations and the proper handling of safety devices accident and hazard alarms as well as first aid kits and emergency rescue equipment The system owner operator will be...

Page 41: ...ting State Indicator on page 31 6 Head mount Head Mount on page 33 7 Barcode reader connection see accessories manual 8 Clamping lever Fig 14 on page 33 9 Nameplate Nameplate on page 27 10 ON Off swit...

Page 42: ...ng On Readiness for Operation on page 47 6 Connection values sticker Summary Table of Operating Data Conditions on page 9 7 Connector panel Connector panel on page 36 8 Nameplate Nameplate on page 27...

Page 43: ...decontamination device UV The blind are part of the enclosure their front face consisting of transparent perspex in order to enable the operator to observe running processes The purpose of the blind...

Page 44: ...d tips Further information Deck Configuration on page 55 The upper level decks decks B and C of the CyBio FeliX are intended for receiving or dispensing of CyBio RoboTipTrays for depositing the protec...

Page 45: ...re lying on Zero deck level height observation is required because of the optimized raster Deck heights Instrument with 55 mm height Deck B C Fig 10 Heights Deck A B and C Please see the illustration...

Page 46: ...detailed information Table 11 Compilation Deck Height Description Command A 115 mm Height 110 mm Usable height accessories B and C 70 mm Height 65 mm Usable height labware All tip types except 1000 l...

Page 47: ...lind are down closed Table 12 Red green LED Table 13 Yellow LED Display Operating state GREEN1 1 OFF The system is not ready for operation System is ready for action Can be operated via control comman...

Page 48: ...The upper LED displays the operating status the lower LED provides an extra pushbutton function for manual operation Table 14 Upper LED Table 15 Lower LED Display Operating state GREEN System is read...

Page 49: ...int 3 Clamping lever Please note The clamping lever Pos 3 Fig 14 must be transferred into front position when no there is no fixed pipetting head a pipetting head may not be pushed in unless the clamp...

Page 50: ...io FeliX pipetting head R This pipetting head provides a mechanism for automated mounting of CyBio RoboTipTrays and liquid handling adapters CHOICETM pipetting head This pipetting head provides a mech...

Page 51: ...Rear side view rear panel removed fan marked The fans are located in the rear position of the system NOTICE The pipettor must be installed in a position that warrants adequate air exchange rates at an...

Page 52: ...the rear panel are the various terminals and interfacing ports Fig 16 Terminals 1 USB jack type B 2 Sub D jack 9 pole COM IN 3 Networking jack Fig 17 Terminals 1 Sub D 9 pole plug COM OUT 2 M8 4 pole...

Page 53: ...IN RS 232 Communication interface HOST PC ETHERNET Network connection NOTE Please consult the manufacturer regarding special interfaces Fig 17 on page 361 1 Special interfaces Description COM OUT Forw...

Page 54: ...wing modes are available for operation PC control Software Controlled Operation on page 56 Manuel mode Control of the Z direction by means of button Pipetting Head Movement in Z Direction on page 53 N...

Page 55: ...destroy individual system components Adequate precautions should be taken to protect all components from environmental impacts mechanical shock or formation of condensed water during transportation o...

Page 56: ...are duly installed 1 Please check that head mount is in mid range position 2 Check that lower deck has moved to rearmost position and upper decks are fully apart 3 Install shipping retainer for the d...

Page 57: ...h related screws and washers The system is in completely packed condition 1 Place pipettor on the bottom of the shipping case 2 Please note recess pocket for handle 3 Use protective foil for covering...

Page 58: ...after arrival of product shipment or not required for a longer period of time it should preferentially be stored in its original packing case Climatic requirements on facilities for system storage are...

Page 59: ...ed by heaters Provide air conditioning for the room if necessary Provide easy access to all system parts at all times and do not obstruct ventilation slots by placing other equipment or fixtures on su...

Page 60: ...protective conductor wiring there is life threatending danger due to electrical shock Insert the main power plug only into a mains socket with a grounded PE contact Make sure that the protection effe...

Page 61: ...duly authorized expert techni cians Initial start up essentially includes Installation and adjustment of system components Providing cable connections and connecting power supply cables Software insta...

Page 62: ...ensure that the pipetting head does not leak liquid A leak test is performed drawing a particular volume of dye solution into the pipette tips and watching their liquid level over a time of 30 minute...

Page 63: ...ates Red steady blinking Internal error is reported error analysis LED Blinks red on page 77 Green steady Reports completion of init routine and readiness for operation 5 Make sure that PC is correctl...

Page 64: ...Turn it on again after a short while Restore PC to operating condition if necessary and trigger empty CyBio Composer method Pipettor performs an init routine Follow instructions on the display regard...

Page 65: ...are recommended to select an offset position 30 mm below uppermost vertical position CAUTION Dangerous situation There is danger of crushing pinching during move to raise or lower the blind1 1 Does n...

Page 66: ...g 19 Head mount with clamping lever in front position 3 Mount pipetting head Fig 20 Insertion of pipetting head 4 Secure pipetting head i e swing clamping lever back again Fig 21 Pipetting head ready...

Page 67: ...osition Fig 22 Pipetting head fixed 4 Take the pipetting head off Fig 23 Removal of pipetting head Fig 24 Head mount with clamping lever in front position 5 Check for proper fixation of the head and c...

Page 68: ...nd electrical move capabilities For manual move please note that move is possible is powered on and powered off state only moderate force should be used to achieve move you should proceed with care El...

Page 69: ...watch pipetting head as you do this head performs move Pipetting head moves into uppermost vertical position moves down again into limit position Stop pipetting head move 1 Release arrow button or For...

Page 70: ...software controlled safe opera tion with the blind in open position for example where apipetteheightinamicroplate needs to be determined or a shift against a defined position be calculate This functi...

Page 71: ...e recommended to use positions 1 2 4 or 5 for tip positioning and waste bin positioning Microplates and reservoirs can be positioned on decks B and C A total of ten positions are available for column...

Page 72: ...in standard mode or with residual eject blow out function Kolben in Nullposition Piston to zero position Pistons move into their zero positions Sp len Mischen Rinse Mix Pipette tips are rinsed or a vo...

Page 73: ...ent of CyBio RoboTipTrays and liquid handling adapters in a selected position Discarding of tips into waste Werkzeuginformation setzen Set tool info Selection of components installed at the pipetting...

Page 74: ...ands to proceed as follows 1 Check that power is available to the pipettor and the PC turned on state 2 Check pipetting head for correct mounting 3 Select Remove command 4 Define a position on deck A...

Page 75: ...follows 1 Select Load command Fig 29 Components window 2 Make setting as indicated below Fig 30 Mounting the transportation lock NOTICE Dangerous situation Remember to install the transportation lock...

Page 76: ...ion referenced to X and Y axis Z axis uppermost vertical position Shift selectable for each axis Fig 31 Positioning window Move to labware Select position referenced to X Y and Z axis Z axis reference...

Page 77: ...For rearward move Y becomes positively signed Example 3 5 mm Following entry of these coordinate positions the pipette tips will be positioned near the upper left edge Depending on the type of plate...

Page 78: ...on lock This is achieved with the help of a Load or a Unload command respectively Only the specified CyBio TipTrays are used in all cases refer to chapter Accessories in the pipetting head manual To p...

Page 79: ...ead is exclusively extended for operation with CHOICE adapters Only column by column line by line and single channel processing capability are available for microplates As a necessary precondition for...

Page 80: ...on deck A After mounting an adapter the information on the attached pipette tips must be set as follows 1 Select Set tool info command 2 Make a selection under the appropriate adapter type If an adapt...

Page 81: ...ing head The same applies to pipette tips with filter filter tips that provide only little net volume Type of tips Designation CyBio TipRack 96 1000 l 1 8 12 Channel CHOICETM Adapter 10 l 1000 l CyBio...

Page 82: ...ette tips need to be placed down in a first step which may be fol lowed by placing down of adapters as necessary In either case a Unload command must be selected To be able to place down tips an adapt...

Page 83: ...ds is adequate for the large majority of acqeous solutions A full volume cycle includes the commands Aspirate Dispense sub volumes where necessary aBlow out step combined with Dispense or separately a...

Page 84: ...in the tip to the dispensed into a reservoir at the end of the process Reverse pipetting is particularly suited where small volumes or foaming or viscous liquid samples have to be processed Where seve...

Page 85: ...ips and or LH adapter down using the function described on Remove of pipette tips liquid handling adapter and CHOICETM adapter on page 66 3 Use On Off switch on the lateral side to turn the CyBio Feli...

Page 86: ...70 2018 CyBio FeliX Operation...

Page 87: ...is may render potential claims for warranty null and void WARNING Danger as a result of electric voltage Observe all safety rules when you inspect cabling for possible faults Error Cause Action for re...

Page 88: ...the error persists you should containt your service contractor or Analytik Jena AG E 5 Kein Spitzenmagazin oder Liquid Handling Adapter vorhanden No tip magazine or liquid handling adapter existent C...

Page 89: ...he method E 24 Kommando zur Zeit nicht ausf hrbar Command not practicable at moment Check software method i e make sure that specifications inputs are in agreement with actually installed hardware If...

Page 90: ...ng adapter not working Notify your service contractor or contact Analytik Jena AG E 39 Unbekannter Liquid Han dling Adapter Unknown liquid handling adapter Turn power supply off Turn on the power agai...

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Page 92: ...for absense of voltage in the pipettor Description Type Rated specs1 1 High Breaking Capacity Fuse Summary Table of Operating Data Conditions on page 9 Line power fuse T 4 A 250V AC 5x20 mm 1 Turn po...

Page 93: ...ndition will create no potential hazards 8 4 4 Manual axis movement The pipetting head can be shifted in X direction with little physical force in manual mode Similarly the decks can be moved with man...

Page 94: ...78 2018 CyBio FeliX What to do if...

Page 95: ...ts Maintenance repair or adjustment of system modules under electrical voltage may only be carried out by a qualified electrician CAUTION Penetrating liquid may cause material damage to electrical and...

Page 96: ...s and hence an increased probability of failure Check for signs of wear and tear on assemblies under mechanical strain and initiate necessary replacements promptly on identifying a case of wear and te...

Page 97: ...st once in a week Clean all liquid holding vessels and the outside of tubes with disinfectant x Perform Head Mount Mainte nance on page 84 x Check O rings at cones for proper condition and replace if...

Page 98: ...formation regarding this topic in chapter Chemical resis tance on page 21 Processing biological samples of a risk group Take particular care when using the device for processing biological samples of...

Page 99: ...applying pressure 8 Wait until all cleaned surfaces have completely dried 9 Reinsert the pipetting head and put all accessories which were removed for clean ing back into the decks 10 Close the blind...

Page 100: ...ways rails must be inspected for clean condition edge burr warping etc 2 An ultra thin layer of silicon grease must be applied on the rail surfaces Wipe off any excessive amount of grease 9 3 3 Cleani...

Page 101: ...ere a maintenance contract has been concluded replacement of O rings will be performed by Analytik Jena AG service per sonnel as part of each on site maintenance package Generally O rings should be re...

Page 102: ...rt moderate pressure and finally remove the O ring mount ing tool and the O ring from the cone 2 If your attempt at O ring removal fails there is still an alternative method for removal of the O ring...

Page 103: ...urn the pipettor on 2 Trigger blind move and make sure that move runs easily unobstructed all currently running move is broken on raising of the blind 1 Turn the O ring mounting tool until the O ring...

Page 104: ...88 2018 CyBio FeliX Maintenance Care...

Page 105: ...using main switch or socket bar for this purpose 5 Disconnect line power cable from socket bar or line power socket 6 Remove all microplates reservoirs and accessories 7 Clean and disinfect the pipet...

Page 106: ...90 2018 CyBio FeliX Shutting Down...

Page 107: ...e factory matched to each other Use only accessories recommended by the manufacturer The manufacturer will refuse any warranty in the event of damage or function failure as a result of system operatio...

Page 108: ...25 441 CyBio RoboTipTray 96 60 l PCR certified pre sterilized OL3810 25 444 CyBio RoboTipTray 96 60 l net volume 18 l PCR certified pre sterilized filter OL3810 25 449 CyBio RoboTipTray 96 40 l OL381...

Page 109: ...terilized OL3811 25 635 S CyBio TipBox 96 50 l PCR certified pre sterilized filter OL3811 25 935 F Head R 96 250 l CHOICETM Head CyBio TipBox 96 250 l PCR certified pre sterilized OL3811 25 637 S CyBi...

Page 110: ...4 755 Description Ordering code number 1 Channel CHOICETM Adapter 10 1000 l OL3316 11 300 8 Channel CHOICETM Adapter 10 1000 l OL3316 11 330 8 Channel CHOICETM Adapter 0 5 50 l OL3316 11 332 12 Channe...

Page 111: ...Head R 384 OL3317 11 380 24 Channel Adapter Head R 384 OL3317 11 390 Description Height mm Ordering code number Comment Support 49 OL3317 11 100 for 384 well tips 60 l and 40 l Support 70 OL3317 11 1...

Page 112: ...ough 96 well DW connection below OL3317 11 180 Tip wash trough 384 well DW connection below OL3317 11 190 NOTE Detailed information about available accessories can directly be obtained from Analytik J...

Page 113: ...fety and environmental provisions of law NOTE Biological samples must be treated in accordance with locally binding regula tions for the handling of infectious material Hazardous substances and relate...

Page 114: ...98 2018 CyBio FeliX Waste Disposal...

Page 115: ...equirements 43 Function Test 46 Functioning 38 Fuse High Breaking Capacity Fuse 9 19 Replacement 76 G Gasoline 82 I Immersion disinfection 23 Incidin Liquid 22 Initial start up 45 Installation require...

Page 116: ...et tool info 57 Shipping retainer 40 Shock indicator 41 Site requirements 43 Software installation 45 Special operating instruction 5 Substances 22 T Transportation lock Packaging 41 Pipetting head 58...

Page 117: ...clean bench with light curtain3 Ordering code number OL5015 505 25 Description page 104 Both automation solutions offer a comprehensive customer protection NOTE Always notice the safety Observe the d...

Page 118: ...nch and door monitoring set1 1 Clean bench 2 CyBio FeliX 3 Door monitoring set Table 1 Components 1 Principe Fig 1 Designation Description 1 Clean Bench Model Safe 2020 Maxisafe 20201 1 Please consult...

Page 119: ...iption 1 Transponder PSEN cs1 11 1 Distance to the switch 20 mm 2 Switch PSEN cs1 1p2 2 LED POWER Fault shows green ready for action 3 Nameplate Nameplate specifications Manufacturer details Product d...

Page 120: ...2 Variant II This variant is equipped with an active optoelectronic protective device safety light curtain Fig 3 Clean bench 1 Clean bench 2 Safety light curtain Fig 4 Safety light curtain1 1 Curtain...

Page 121: ...el Safe 2020 Maxisafe 2020 1 Principe variant OL3317 23 460 Fig 4 Designation Description 1 2 Light curtain 3 Protected area For CyBio FeliX 30 5015 500 24 4 Nameplate Nameplate specifications Manufac...

Page 122: ...n Maschinen Allgemeine Gestaltungsleits tze Risikobeurteilung und Risikominderung Safety of machinery General principles for design Risk assessment and risk reduction DIN EN 61010 1 2011 07 Sicherheit...

Page 123: ...kroom must be limited To minimize the risk of injury by moving parts the use of an external safety device is highly recommended For connection of the external safety device to the CyBio FeliX enclosur...

Page 124: ...on An opening switching element between PIN 2 and PIN 4 interrupts the current flow re sulting in an E STOP Designation Description comment PIN 1 5 V power PIN 2 Receiving line signal line PIN 3 GND P...

Page 125: ...n auf begr ndetes Verlangen die speziellen Unterlagen zu dem unvollst ndigen Produkt ber unsere Dokumentationsabteilung zu bermitteln Das unvollst ndige Produkt darf erst in Betrieb genommen werden we...

Page 126: ...110 2018 CyBio FeliX...

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