86
Gentier Real-Time PCR System
a.
Delta T
m
Discrimination
: the default Delta T
m
discrimination sensitivity provided by the
amplification software is 0.5; reduce this value, the application software will grouping the
melting curve at a higher temperature resolution.
b.
Curve Shape Discrimination
: the default curve shape discrimination sensitivity provided
by the amplification software of is 0.5; reduce this value, the application software will
grouping the melting curve at a higher shape resolution.
▶
Other:
a.
Pos/Neg Threshold
: user can use
and
keys to set the threshold value for the
application software to detemine the positivity or negativity of samples
b.
Temperature Compensation
: user can check
Temperature Compensation
check box and
the application software will automatically calculate the temperature compensation value
between sample block wells. User can use
and
keys to change the temperature
compensation value.
c.
Baseline Group
: user can set the baseline group gene for the
Diference Plot
functional
module.
II.
Gene and Sample
tab: the introduction of relevant parameters and function keys is same
with those introduced in
D-3.3.4.1Abs Quant - Abs QuantAnalysis Setting.
3.3.4.5 Genotyping
The Genotyping analysis adopts two sequence specific probes labeld with different dyes to
identify the wild type and mutantion alleles, respectively. After running the experiment
program, the application software will automatically detect the end point fluorescence and
distinguish different genotypes based on the distribution of two dyes on the scatter plot.
The Genotyping analysis interface consists of seven functional modules:
Amplification Curve,
Scatter Plot, Sample Setting, Result Table, Raw Curve, Raw Fluorescence
and
Heat Map
. The
Genotyping analysis interface is divided into four areas, the application software displays four
functional modules (
Amplification Curve, Scatter Plot, Sample Setting, Result Table
) by
default, as shown in figure D 16.
Reminding
: the parameter descriptions of
Amplification Curve, Sample Setting, Raw
Curve, Raw Fluorescence
and
Heat Map
functional modules are same with those
introduced in
D-3.3.4.1Abs Quant
.
