4
Gentier Real-Time PCR System
3.1.2 Instrument Technical Parameters
Thermal Parameters
▶
Temperature Accuracy
: ≤ 0.1°C;
▶
Temperature Uniformity
: ± 0.1°C;
▶
Temperature Precision
: ≤ 0.1°C;
▶
Max Heating and Cooling Ramp
: 6.1°C/s
Optical Parameters
▶
Excitation Light Source
: LED light sources;
▶
Detect System
: photodiodes;
▶
Optical Channel
:
Dyes
Instrument Models
96E
96S
96R
96C
1
FAM, SYBR Green I ,etc.
2
VIC, HEX, TET, JOE, etc.
3
ROX, Texas Red, etc.
4
Cy5, etc.
5
Alexa Fluor 680, etc.
6
FRET Probe
Reminding
: channel 6 is for FRET probe, which takes the report group of channel 1 as the
energy donor and the report group of channel 2 as the energy receptor.
Detective Parameters
▶
Throughputs
: simultaneously detect 96 samples.
▶
Repeatability
: CV ≤ 0.5%.
▶
Linear Correlation
:
│
r
│
≥ 0.999, within the scope of no less than ten magnitudes
concentration gradients.
▶
Throughputs
: simultaneously detect 96 samples.
