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Operating Instructions UVD 170S/340S 

 

 

20

Standard Flow Cell with PEEK Capillaries (Order no. 5065.1820) 

The standard flow cell with PEEK capillaries has a volume of 10

µ

l and a path length of 9mm. 

Inlet and outlet are PEEK capillaries. The flow cell is 100% biocompatible. The maximum 
pressure rating of the PEEK flow cell is 100bar (10,000kPa). 

 

  Important:

 

When cleaning the flow cell (

 section 6.3.4), please note that only the 

lens retaining plates and the lenses may be removed. 

Do not

 attempt to 

remove the flow cell body from its housing! Clean the flow cell body 
together with the housing. 

 

Preparative Flow Cell (Order no. 5065.1800P) 

The preparative flow cell for the 

UVD 170S/340S 

has a volume of 6

µ

l and a path length of 

2mm. The cell body is composed of PEEK and is thus 100% biocompatible. The maximum 
pressure rating of the flow cell is 100bar (10,000kPa). 
 

  Important:

 

When cleaning the flow cell (

 section 6.3.4), please note that only the 

lens retaining plates and the lenses may be removed. 

Do not

 attempt to 

remove the flow cell body from its housing! Clean the flow cell body 
together with the housing. 

 

Micro/Glass Capillary Flow Cell  (Order no. 5065.1800M) 

The micro flow cell has a volume of 0.14 

µ

l at a path length of 8 mm. The flow cell body is 100 

% biocompatible. The maximum pressure rating of the micro flow cell is 400bar (40,000kPa).

 

 

  Important:

 

Please note that it is 

not possible to disassemble the micro flow

 cell for 

cleaning. Clean the micro flow cell by flushing with 0.2M nitric acid, 
followed by water and methanol. 

Summary of Contents for UVD 170S

Page 1: ...UV VIS Detector UVD 170S 340S Operating Instructions Version 3 01 Date July 14 2000 Dionex Softron 2000 Doc UVD170SUVD340_OI_E_V301 doc...

Page 2: ...ric emissions standard Part 1 Residential commercial and light industry EN 50082 1 1992 Electromagnetic Compatibility EMC Generic immunity standard Part 1 Residential commercial and light industry EN...

Page 3: ...n the Front Panel 8 4 3 Installation and Operation within CHROMELEONTM 9 4 3 1 Communication Port 9 4 3 2 Required CHROMELEONTM Modules 9 4 3 3 Installation with the CHROMELEONTM Installation Tool 9 4...

Page 4: ...and labour ex works Please note that wear parts i e needle needle seat syringe and valve seals cannot be covered by the warranty The warranty period for the deuterium lamp is six months from delivery...

Page 5: ...accompanying information may result in damage to the instrument Warning Indicates that failure to take note of the accompanying information may result in personal injury 1 4 Intended Use of This Instr...

Page 6: ...The alteration procedure is as follows Switch off the unit and disconnect the power cable Draw out the fuse cartridge using a small screwdriver fig 1 Using small pliers tweezers to pull out the small...

Page 7: ...using a small screwdriver fig 1 replace fuses as appropriate Important Only use fuses as indicated in fig 1 min voltage 250 V or those listed in the original parts list If using US type fuses follow t...

Page 8: ...hnical Specifications page 23 For the standard flow cell the pressure rating is 100 bar 10 000kPa Important The flow cell is primed with 2 propanol 2 4 Rear Panel Connections The UVD 170S 340S is cont...

Page 9: ...installation Warning Ensure that the PC is off and the power cable disconnected before attempting installation Important The interface board must be protected against static charge at all times If di...

Page 10: ...bined with extremely low noise electronics enable highest sensitivity Simultaneous detection on 4 wavelengths recording of 3D data fields and the comprehensive data analysis capabilities within the CH...

Page 11: ...and allow 5 minutes before switching the deuterium lamp on again Otherwise the lamp becomes too hot and could be damaged message Lamp too hot In the event that the PC is switched off or the connection...

Page 12: ...d then the CHROMELEONTM Installation Tool Server Configuration Select the timebase to which you want to assign the detector UVD170S 340S Via the Edit menu or the menu of the right mouse button choose...

Page 13: ...ge to view the following signal parameters Signal parameter Description Signal Name Signal name Unit Signal unit of the recorded raw data Factor current amplification factor Tab Dialog Box Error Level...

Page 14: ...signal specific and global commands are available Signal specific commands Signal type Parameters UV channel Step Average Wavelength Bandwidth Ref Wavelength Ref Bandwidth Signal type Parameters 3D Fi...

Page 15: ...yl acetate Ensure for the calibration that the deuterium lamp is already warm because its spectrum changes much during the first minutes after switching on the lamp If these conditions are not met the...

Page 16: ...r not switched on Switch on detector Fuse blown Replace fuse Replaced fuse blows immediately Call Dionex service Connecting cable between UVD 170S 340S and PC not correctly installed Connectors on the...

Page 17: ...voir on the same level as flow cell Wrong wavelength Select suitable wavelength Connecting cable between UVD 170S 340S and PC not correctly installed Connectors on the detector and the PC must be inse...

Page 18: ...in the original packing If no original packing is available this can be acquired from Dionex or your local Dionex representative Please note The warranty coverage shall become invalid in any case ide...

Page 19: ...Operating Instructions UVD 170S 340S 16 Fig 6 Side view UVD 170S 340S side panel open...

Page 20: ...2 5mm Allen key and carefully remove the lamp The Allen key is contained in the Accessories Warning During operation the lamp becomes extremely hot and remains so for some time after the unit is swit...

Page 21: ...glass capillary flow cell order no 5065 1800M Standard Flow Cell Order no 5065 1810 The standard flow cell of the UVD 170S 340S has a volume of 10 l and a path length of 9mm The flow cell body is com...

Page 22: ...lens 1343 0511 9 Lens retaining plate2 5065 1812 11 Single part hand tight fitting included in 5065 1815 12 Capillary tube 1 58x0 25 ID included in 5065 1815 13 PEEK double ferrule 2261 0122 Order No...

Page 23: ...he UVD 170S 340S has a volume of 6 l and a path length of 2mm The cell body is composed of PEEK and is thus 100 biocompatible The maximum pressure rating of the flow cell is 100bar 10 000kPa Important...

Page 24: ...low cell cover Replace the side panel The detector may now be switched on and communication with CHROMELEONTM restored using the Connect command 6 3 4 Flow Cell Cleaning Important In contrast to the o...

Page 25: ...ts as this may lead to irreparable damage Replace the lenses onto either side of the flow cell body so that the flat faces are on the inside Replace the retaining plates 6 and 9 taking care to tighten...

Page 26: ...l 9mm path Preparative flow cell 6 l 2mm path Micro capillary flow cell 0 14 l 8mm path Flow cell pressure rating Standard flow cell 100bar 10 000kPa Standard flow cell PEEK cap 100bar 10 000kPa Prep...

Page 27: ...1051 Spanner x 5 16 1 2146 2625 Allen key 2 5mm 1 2261 0121 PEEK fitting screw 1 16 15 mm 2 2261 0122 PEEK double ferrule 2 2309 1100 Accessories container 1 6007 9100 Silicon tubing 2 8 x 1 3 3m 1 8...

Page 28: ...M3x23 DIN 965 3 pcs Quartz lens 2 pcs Lens retaining plate1 1 pc Lens retaining plate2 1 pc 6065 9001 UVD 340S 170S Repair Kit Quartz lenses 2 pcs PEEK capillary tubing 1 16 0 50 mm 2 m PEEK fitting...

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