Document Title:
Remote Tomo5 operation guide for eBIC microscopes
Author(s):
Kyle Morris
Julika Radecke
Andrew Howe
Version:
2.0.3
Date:
27 Jun 2022
If you have any questions at any point during set-up, please ask your Local Contact for assistance Page 26 of 29
5.2 Start automated acquisition
5.2.1 Check your tomography acquisition parameters
Tomography > Data Acquisition
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Set up data acquisition parameters:
o
Tilt step, max positive and negative angle, tracking scheme
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Standard settings are shown in the image below.
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Please leave the “Adjust ZLP” button unchecked as this might fail and has just been
calibrated using the AutoFunctions
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Please select ‘Close Column Valves’
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You may wish to select ‘Skip Eucentric’ if you have refined positions or your grid is flat
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You may wish to select
‘Adjust Exposure Time’ if you want to increase your dose at
higher tilts
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You may also select specific positions for collection
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You may also ‘Update Defocus’ to batch modify the defocus series of a set of batch
positions
You should refer to your dose calibration in 3.1.5 to double check those collection parameters for your
Exposure Preset are reasonable.
From your calculation, will each tilt receive sufficient dose for the imaging and tracking to work?
If you are unsure, you could move to an unused area and acquire test data acquisition images at zero and
increasing tilts that reflect your collection.
!