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USER MANUAL

Cat. No. B03000002

IP-Star

®

Compact

Fully Automated System for Epigenetic Applications

Version 1

I

08.2016

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Summary of Contents for B03000002

Page 1: ...USER MANUAL Cat No B03000002 IP Star Compact Fully Automated System for Epigenetic Applications Version 1 I 08 2016 ...

Page 2: ...expenses to return the repaired or replacement product This warranty covers only manufacturing defects and does not cover any damage caused by misuse lack of compliance to recommendations stated in the manual neglect accidents abrasion or exposure to extreme temperatures chemical solvents or acids We strongly recommend that maintenance or repairs of Diagenode s products are performed by our approv...

Page 3: ...ponents 10 Front View 10 Right Side View 10 Internal Components 11 Installation 13 Accessories and Disposables 14 Accessories 14 Disposables 15 Disposables delivered by Diagenode 15 Reagents 16 Basic for Hardware Operation 17 Front Door Operation 17 Putting On and Taking Out Waste Tip Box 17 Setting Racks and Disposables in Place 18 Setting PCR Plate onto Heat Cool Unit 19 Basic Software Operation...

Page 4: ...k nearby the instrument a quick reference for all the operators as needed Do not operate the instrument without sufficiently understanding instructions described in this book Always keep this book nearby for easy reference at any time Below is a list of the different devices included in the Instrument Package No Description Quantity Remarks 1 SX 8G Compact Main Body 1 2 Grip of Front Door 1 w two ...

Page 5: ...s in operating the instrument for your safety according to the description in this book Pay close attention in handling samples with high risk of biohazard When operating the instrument under low environmental temperature keep the instrument powered on Be sure no foreign materials get into the instrument from outside Do not scratch or press the operation panel using knives or materials with sharp ...

Page 6: ...xtraordinary heat smoking abnormal smell etc immediately unplug the power cable from wall outlet to avoid fire or electric shock to you Please contact us for technical support If foreign material or water is splashed inside the instrument immediately unplug the power cable from wall outlet to avoid fire or electric shock Please contact us for technical support If power cable becomes damaged core w...

Page 7: ... software Simultaneous processing of up to 8 samples by 8 nozzles Performance Instrument type Desktop Throughput Samples 1 8 samples batch Processing time About 30 min 1 8 samples Temperature Control Two Heat Cool Blocks 4 95 C Handling Volume 5 200 µl Dispensing Accuracy 5 10 µl less than CV 20 11 20 µl less than CV 10 21 200 µl less than CV 2 Accuracy with distilled water at room temperature 20 ...

Page 8: ...More than 70kPa min Transport Conditions Temperature 25 60 C Relative Humidity 15 75 Non Condensing Barometric Pressure More than 70kPa min Environmental Requirements Temperature 15 30 C Relative Humidity 15 75 Non Condensing Altitude Up to 2 000 m Space Keep distances from walls as shown below for ventilation maintenance work etc Also keep more than 200mm space over the instrument Wall SX 8G Comp...

Page 9: ...he instrument on horizontal surface Do not place the instrument where it will get direct sunlight or vibration When operating instrument in a cold chamber or cold room keep the instrument power on to avoid condensation The part where this label is attached becomes hot Be sure not to touch while working The part where this label is attached inside cover has the possibility of biohazard contaminatio...

Page 10: ...strument The Power Switch and Communication Port Connector are located at the front and Power Inlet Connector and Fuse Unit are located at the back Note Replacement of the fuse shall be made by field service personnel authorized by Diagenode Contact us for fuse replacement Waste Tip Box Access Door Front Door Operation Touch Panel Power Lamp Front Back Power Switch Communication Port Connector RS ...

Page 11: ...th accurate and rapid aspiration dispensing Magnet Eight magnets are aligned with 8 tips to simultaneously separate magnetic particles for up to 8 samples 2 Stage Heat Cool Unit Two Heat Cool Units are equipped and can hold 8 and 12 tubes stripes Temperature can be set for 4 to 95 C depending on protocols Rack Holder Racks and Disposables to be used vary by protocols Refer to Application Manual fo...

Page 12: ...ontainer M or L Reagent Tip Rack 1 Holds 12 Tips for single pipetting and 12 Screw Capped Tubes of 1 5ml or 2 0ml Reagent Tip Rack 2 Holds 8 Tips for single pipetting 4 Screw Capped Tubes of 1 5 ml or 2 0 ml and 4 Screw Capped Tubes of 5 0 ml Microtiter Plates Holds 96 Wells Microplates Waste Chute Place where waste tips etc are discarded Note The above layout is an example and it could vary depen...

Page 13: ...s Then replace Right Side Cover and fix it by two screws 7 Open Left Side Cover by removing 2 fixing screws 8 Remove Y Axis fixing jig to left side base by removing 4 fixing screws Then replace Left Side Cover and fix it by two screws 9 Connect Power Cable to Power Inlet Connector on the right side of the instrument Then turn Power Switch on assuring that Front Door and Waste Tip Box Door are clos...

Page 14: ...AC110V 1 O ring 8 Silicon Grease 1 Fuse 1 96 Tip Rack It holds Tips up to 8x12 pieces Reagent Reservoir Rack It holds up to 8 Medium Reagent Container or up to 4 Large Reagent Container Container L is double sized of Container M Reagent Tip Rack 1 for 1 5 2 0ml tube It holds 12 Tips for single pipetting and 12 Screw Capped Tubes of 1 5ml or 2 0ml Reagent Tip Rack 2 for 1 5 2 0 5 0ml tube It holds ...

Page 15: ... SX 8G Compact Reagent Reservoir Rack WA 007 0020 Large Reagent Container for SX 8G Compact Reagent Reservoir Rack WC 001 1000 Tips bulk 96 Tip Rack Reagent Tip Rack 1 2 WC 002 0960 Tips box 96 Tip Rack Reagent Tip Rack 1 2 WA 003 0010 96 well microplates Microplates Module WA 001 0080 200 µl tube strips 12 tubes strip cap strips Heat Cool Units WA 002 0120 200 µl tube strips 8 tubes strip cap str...

Page 16: ...iagenode com Reagents Refer to each Application Manual for reagents to be used and directions for use Do not reuse any disposables reagents to avoid contamination or instrument malfunction Do not use disposables reagents other than specified to avoid instrument malfunction When discarding disposables follow the instructions defined by your facility or regional laws ...

Page 17: ...r it completely finished its operation Putting On and Taking Out Waste Tip Box Gently open and close the access door to avoid damage Waste Chute Waste Chute can be removed by taking it out from Waste Tip Discard Hole for cleaning Waste Tip Box Waste Tip Box can be removed for discarding Tips or for cleaning Open Front Door first and then open access door for Waste Tip box Take out Waste Tip Box by...

Page 18: ...es on front side When Racks are not correctly positioned Tip pickup failure inaccurate aspiration dispensing or instrument malfunction may occur When Reagent Tip Racks 1or 2 are not correctly positioned Tips will not be picked up resulting instrument malfunction Setting 96 well microplates Set Microtiter Plates in positions designated by the selected application taking care of positioning directio...

Page 19: ...ol Unit taking care of the positioning direction and push it down to fit completely Cut 1 2 3 A B C When PCR Plate is not positioned correctly adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purification If non recommended PCR plate is used adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purif...

Page 20: ...e Tip Box and 7 1 Front Door Operation Turn on Power Switch located on the right side of the instrument When the instrument is turned on the Touch Panel screen is activated Shut Down Turn instrument power off making sure that the instrument is not operating If it is operating stop operation by pressing Protocol Stop button Touch Panel Operation Using Stylus Pen select desired function Operate Touc...

Page 21: ...een disappears the Diagenode splash screen is displayed for several seconds and then disappears Start Screen Top menu After the Digenode splash screen disappears the start screen is displayed This is the first active window it allows the user to enter into three different parts of the software USER ACTIONS Buttons Protocols Maintenance Information Protocols screen All available protocols are displ...

Page 22: ...shall turn executable Buttons The user presses the Back button Returns the user to the display of the Protocols screen they came from The user presses the Shutdown button Then screen shall be changed to Power Off The user presses the Run button Then screen shall be changed to Sample number Page up the list box Page down the list box Defined protocol name lists Screen Sample number After the user p...

Page 23: ...Protocol List screen they came from The user presses the Next button Then screen shall be changed to Layout information The user presses the Save Parameter button Then screen will be changed to Save Parameter Confirmation OK Current parameters shown in the Display View will be stored to the Protocol ptd And returns the user to the display of the Configuration screen No Returns the user to the disp...

Page 24: ...en the Layout Information screen appears Buttons The user presses the Back button Returns the user to the display of the screen they came from The user presses the Next button Then screen shall be changed to Set confirmation When the user presses a block that block is magnified on the work surface layout background The magnified view provides a better display of the correct method setup for that b...

Page 25: ...ey came from The user presses the Run button This is the expected action when user gets to this display after reviewing blocks Runs the protocol Screen Running After the user presses the Run button in the Set confirmation screen the Running screen appears Buttons The user presses the Stop button Then screen shall be changed to Stop Dialog Status screen is preferred as a progress bar that moves acr...

Page 26: ...he Startup screen When OK is pressed then the Startup screen appears and the user can immediately begin to remove their sample and prepare for the next run At this point user is expected to be ready to press RUN Buttons The user presses the OK button Then screen shall be changed to Categories Name Protocol List Stop Dialog Buttons The user presses the Stop button Then screen will be changed to Fin...

Page 27: ...tion Unit should be cleaned after every run Cleaning Procedure Stage Magtration Unit All Racks Waste Chute Waste Tip Box In addition to the list above the inside of the instrument and the accessories should be cleaned after each use Cleaning Method Choose cleaning liquid depending on the contamination status and wipe the contaminated surface using paper such as Kim Wipe moistened with the cleaning...

Page 28: ...in proper tip attachùent and avoid liquid leakage 1 Remove old grease 2 Wear gloves and put small amount of Silicon Grease on the fingertip Then spread Silicon Grease on fingertips by rubbing fingertips together 3 Put Silicon Grease on O rings black rubber rings on Nozzles 4 Place a tip on the nozzle to spread the grease evenly around the O ring Be sure not to put an excess of Silicon Grease as it...

Page 29: ...he same error occurred in the next run of the protocol Contact Diagenode when countermeasure taken does not resolve the problem Suspending or Aborting Protocol Run When you want to suspend a protocol run operation select Suspend button in the display to suspend instrument operation It may take some time before suspending its operation depending on the process under execution Select START button to...

Page 30: ... Be sure to confirm appropriate setting of reagents and disposables before starting protocol run If you want to add disposables after protocol started press STOP to abort instrument operation It is strongly recommended to restart the protocol run from the beginning after completion of adding disposables If you want to continue the protocol run from halted point after adding disposables you will be...

Page 31: ... restarted from the beginning after replacing all disposables needed 200008 Axis Endlimit error Axis other than Z was moved beyond its movable range 200001 Axis error Error other than errors described above Note The errors listed above are usually caused by inadequate setting of disposables accessories or if the door is not shut properly Be sure to check the placement of accessories disposables an...

Page 32: ...PE LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Belgium Tel 32 4 364 20 50 Fax 32 4 364 20 51 orders diagenode com info diagenode com Diagenode Inc USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 USA Tel 1 862 209 4680 Fax 1 862 209 4681 orders na diagenode com info na diagenode com For a complete listing of Diagenode s international distributors visit http www diagenode c...

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