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OligoPilot 400

Operating Instructions

Original instructions
Translation disc included

Summary of Contents for OligoPilot 400

Page 1: ...OligoPilot 400 Operating Instructions Original instructions Translation disc included cytiva com...

Page 2: ...andcommunicationconnections 55 3 3 8 Inertgasdeliverysystem 58 3 4 Containersforreagentsandsolvents 60 3 5 Columns 61 3 6 Flowchart 62 3 7 UNICORN controlsystem 64 4 Installation 65 4 1 Unpackingandtr...

Page 3: ...ctinformation 136 8 5 2 EuropeanUnionandEuropeanEconomic Area 137 8 5 3 EurasianEconomicUnion 138 8 5 4 RegulationsforNorthAmerica 140 8 5 5 Regulatorystatements 141 8 5 6 DeclarationofHazardousSubsta...

Page 4: ...tion descriptionsofsafetynotices intendeduseof OligoPilot 400 andlistsofassociateddocumentation Inthischapter Section Seepage 1 1 Importantuserinformation 5 1 2 Aboutthismanual 7 1 3 Associateddocumen...

Page 5: ...edinanyclinicalprocedures orfordiagnosticpurposes WARNING Donotoperatetheproductinanyotherwaythandescribedinthe userdocumentation Prerequisites Inordertooperate OligoPilot400 safelyandaccordingtothein...

Page 6: ...rdoussituationwhich ifnotavoided couldresultinminorormoderateinjury Itisimportantnotto proceeduntilallstatedconditionsaremetandclearlyunderstood NOTICE NOTICEindicatesinstructionsthatmustbefollowedtoa...

Page 7: ...way Scopeofthismanual ThisOperatingInstructionmanualisvalidfor OligoPilot400 Typographicalconventions Softwareitemsareidentifiedinthetextbybolditalictext Hardwareitemsareidentifiedinthetextbyboldtext...

Page 8: ...eneralSpecifica tion GS Technicaldataforthesystem AssemblyDrawing AD Physicallayout Provides dimensionaldata BillofMaterial BOM Descriptionofprocess related components includingwetted materialsandspec...

Page 9: ...Themanualscontaindetailedinstructionson howtoadminister UNICORN workwith methods performrunsandevaluateresults TheOnlinehelpcontainsdialogdescriptionsfor UNICORN TheOnlinehelpisaccessedfromthe Helpme...

Page 10: ...ergencyandrecoveryprocedures Inthischapter Section Seepage 2 1 Safetyprecautions 11 2 2 Labels 23 2 3 Emergencyprocedures 27 Important WARNING Beforeinstalling operatingormaintainingtheproduct all use...

Page 11: ...eproductandtheoperationalprocessesmayhaveontheclassi ficationofthehazardousarea Theprocessmightcausetheareato increaseorthezoneclassificationtochange Implementtherisk reductionmeasuresneeded including...

Page 12: ...ayresultinhighpressuresremainingin thesystemflowpath WARNING Beforeconnectingacolumn readtheinstructionsforuseofthe column Toavoidexposingthecolumntoexcessivepressure make surethatthepressurelimitisse...

Page 13: ...oductusespotentiallyexplosiveliquids Consultlocalauthoritiesregardinglocalrulesandregulations beforeinstallingoroperating WARNING Explosionhazard Afterclosedownofthesystem makesurethat thereardooriscl...

Page 14: ...athandcreateanexplosiveatmos pherewhenmixedwiththevaporizedsolvent Personalprotection WARNING PersonalProtectiveEquipment PPE Wheneverpacking unpacking transportingormovingtheproduct wear Protectivefo...

Page 15: ...tingequipmenthas thecapacitytosafelyliftthecrateweight Makesurethatthecrate isproperlybalancedsothatitwillnotaccidentallytipwhenmoved WARNING Heavyobject Thecraterampisnotreinforcedinthecenter Do notu...

Page 16: ...sopropanol and80 H2O water atdelivery Thedenaturedalcoholmixturecanbehazardoustohumans ifconsumed Flushoutthedenaturedalcoholbeforeassembling testingorinte gratingtheproductintotheintendedprocessconte...

Page 17: ...ctpowerfromtheinstru mentbeforereplacingfuses WARNING Forcontinuedprotectionfromfirehazard replaceonlywithsame typeandratingoffuse WARNING Accesstopowerswitchandpowercord Thepowerswitch mustalwaysbeea...

Page 18: ...S outside therange100to240V ForULmarkedsystems donotuseaUPS supplyexceeding120V CAUTION ConnectionofanexternalUPSforsystemoperationshallonlybe performedbyauthorizedpersonneltoavoidmismatchingor connec...

Page 19: ...lacesolventcontainersontopofthevalvedoor Ifthisis done containersmayfalldownwhenthevalvedoorisopened WARNING Onlyusecolumnsthatwithstandexpectedpressures Ifnot the columnmightrupture resultingininjury...

Page 20: ...exposedtopotentiallyhazardoussubstances makesurethatthe OligoPilot400 isproperlydecontaminatedandsanitizedbefore maintenanceorservice WARNING Allrepairsshouldbedonebyservicepersonnelauthorizedby Cyti...

Page 21: ...tainingorservicing OligoPilot400 WARNING Decontaminatetheequipmentbeforedecommissioningtoensure thathazardousresiduesareremoved WARNING ReleasetheInertgaspressureinthebottlesbeforeattemptingto replace...

Page 22: ...ingsystemmustbetestedforleakageat maximumpressureforcontinuedprotectionagainstinjuryrisks duetofluidjets burstpipesorexplosiveatmosphere 2 Safetyinstructions 2 1 Safetyprecautions 22 OligoPilot400Oper...

Page 23: ...or OligoPilot400 TheEXmarkandthetypecertificatenumberonthesystemlabelshowthatthesystem fulfillstherequirementsoftheEuropeandirectiveforequipmentintendedforusein potentiallyexplosiveatmospheres ATEX201...

Page 24: ...era ture Pneumaticsupply Pneumaticsupply Minpneumaticflowrate Minimumpneumaticflowrate Minamountofpurgeair Minimumamountofpurgeair Theleastair volumethatmustbetransferredthroughthe cabinettoreachthesa...

Page 25: ...ategoryisdesignedto becapableoffunctioninginconformitywiththe operatingparametersestablishedbythemanu facturerandensuringanormalprotectionlevel Equipmentofthiscategoryisintendedforusein areasinwhichex...

Page 26: ...for 8 min at a flow rate of 450 l m WARNING Powermustnotberestoredafterenclo surehasbeenopeneduntilenclosurehasbeenpurged for8minataflowrateof450L m WARNING PROTECTIVE GAS SUPPLY VALVE This valve must...

Page 27: ...lot400 in thiscase Precautions WARNING Emergencyshutdownmayresultinhighpressuresremainingin thesystemflowpath WARNING Emergencystop PressingtheEMERGENCYSTOPdoesnot automaticallydepressurizetheflowpath...

Page 28: ...thelocalsafetysystemisconnected itautomaticallyshutsoffanyexternal mediasupply suchasacetonitrile thereby preventingexplosiveliquidsfromleaking out 2 Ifrequired alsoswitchoffthemainspower supplyusingt...

Page 29: ...eumatic signal needs to be blinded using a 6 mm plug Inaddition anexternalsignalcanbeconnectedtothejunctionbox suchasanalarm signalfromthefumehoodventilationoradoor Whenthealarmsignalisissued itcan be...

Page 30: ...ecommunicationerroronthedisplays Therun willcontinueontheprocesssystemanddatawillbe savedintheCU 960 Whenthecomputerisrestarted datawillberecoveredfromtheCU 960 UniNetnetworkinterruption Intheeventofi...

Page 31: ...ructionsbelowtorestart OligoPilot400 afteremergencyshutdownor powerfailure Step Action 1 Checkthatthecompressedairsupplyisturnedon 2 Iftheprocesssystemisnotturnedon turntheMAINSSWITCH locatedon thejun...

Page 32: ...ts includingthe UNICORN controlsystem Inthischapter Section Seepage 3 1 Overview 33 3 2 Illustrationsof OligoPilot400 34 3 3 Systemcomponents 42 3 4 Containersforreagentsandsolvents 60 3 5 Columns 61...

Page 33: ...ide Note Completely anhydrous conditions are essential for the efficiency of this step 3 Oxidationofthephosphitetriestertoaphosphotriesterusingiodine Phos phorothioatelinkagescanbeintroducedatthisstag...

Page 34: ...tion Systemoverview Theillustrationbelowshowsafrontviewofthesystemwithalloptions 3 2 4 1 5 Part Description 1 Processcabinet 2 Columnholder 3 Standforcontainersforreagentsandsolvents 4 Manifold 5 Tilt...

Page 35: ...open 1 2 3 4 5 6 7 8 9 Part Function Description 1 Valve XV 051 ColumnInletValve 2 Valve XV 023 ReagentValvePumpP 201B 3 Valve XV 022 ReagentValvePumpP 201B 4 Valve XV 021 ReagentValvePumpP 201A 5 Val...

Page 36: ...Valve XV 001 Amidite1Valve PumpsandSensors TheillustrationbelowshowsthepumpsandsensorswithintheProcessCabinet The illustrationislocatedatthefrontoftheProcessCabinetwiththedooropen 1 2 3 4 5 7 8 9 6 P...

Page 37: ...sCabinet 7 UVcellAE 131 8 ConductivitycellCE TE 101 9 PressuresensorPT 111 Junctionbox TheillustrationbelowshowsthefrontoftheJunctionboxwithalloptions 4 5 1 2 3 SERVICE SWITCH MAINS SWITCH PURGE ALARM...

Page 38: ...tchlocatedonthefrontoftheJunction Box 1 Servicemode nopurgerequired 0 Off 2 Normalmode purgerequired WARNING Explosionhazard ServiceorMaintenanceof OligoPilot400 must beperformedinanon classifiedarea...

Page 39: ...LARM indicator Red green Systemandjunc tionbox Theindicatorisredafterpoweringup thesystemandremainsreduntilthe prepurgingprocedureoftheprocess cabinethassuccessfullyfinished Aftersuccessfulpurge thein...

Page 40: ...onbox 2 Pneumaticsupply 3 OpticalEthernetcommunicationfromjunctionbox 4 Inertgasinletconnection 5 Purgecontrolsignaltothejunctionbox 6 OpticalPROFIBUS industrystandardcommunicationtothejunction box 7...

Page 41: ...netpurgecompleted 2 Ethernetcommunicationfromthecomputer 3 Externalsignals 4 OpticalPROFIBUScommunication 5 OpticalEthernetcommunicationwiththesystem 6 Powersupplyfromthejunctionboxtothesystem 7 Mains...

Page 42: ...section Section Seepage 3 3 1 Cabinetpurgingsystem 43 3 3 2 Systempumps 45 3 3 3 Metersandsensors 48 3 3 4 Valves 52 3 3 5 Tubing 53 3 3 6 Cooler 54 3 3 7 Powerandcommunicationconnections 55 3 3 8 Ine...

Page 43: ...oltage Afterthesystemcabinethasbeensuccessfullypurged voltageissuppliedto thesystemaswell Overridingthisinterlockisonlypossiblebyusingtheserviceswitch Purgingmodes Thepurgingsystemoperatesintwomodes 3...

Page 44: ...allygoestopurgingmodeandthepowerindicator onthejunctionboxandthesystemisturnedon Purgingmode Purgingmodeisusedundernormaloperationtomaintaina certainoverpressureinthecabinettopreventflammable gasesfro...

Page 45: ...ionmodeandcreatespulsesintheflowrate whichisnormalfortheapplication Thepumpsareequippedwithpressuresensorslocatedintheoutletmanifold P 201A andP 201BshareacommonsensorconnectedtopumpmoduleP 201A Thepr...

Page 46: ...01AandP 201B P 201A P 201B P 202 Flowrate 800mL min Note P 907 and P 908together can produce a total flow rate up to 1200 mL min 400mL min Pressure range 0to15bar 0to15bar 3 Systemdescription 3 3 Syst...

Page 47: ...ads Thefollowingflowdiagramshowsthetubingconfigurationofthepistonrinsing system A B P 907 P 908 1 2 3 Part Function 1 Rinsingsolutionacetonitrile 2 Three portvalve 3 Three portvalve 3 Systemdescriptio...

Page 48: ...adeningand timedelaybetweenthedetectors Theflowcellsareeasilyaccessiblefromthefront paneltofacilitatemaintenance Conductivitymeter Theconductivitymeterprovidesaccurate inlinemonitoringofconductivityan...

Page 49: ...berstogetherwiththeflowcellprovideahighsignal tonoiseratiowithminimaldriftandrefractiveindexeffects Thewavelengthcanbechangedusingthe UNICORN softwareduringarun eitherfrom themethodorbymanualinstructi...

Page 50: ...manifoldblocksoftherespectivepumps Thethirdpressuresensor PT 111 islocatedafterthecolumn Thepressurerangeis0to25bar 2 5MPa 362psi Part Function 1 PressuresensorPT 201 2 PressuresensorPT 111locatedafte...

Page 51: ...rdertomaintainagooddistributioninthecolumnandtoavoidcavitationin theUVcell Thebackpressurecanbecontrolledintwoways eitherbydefiningavalvepositionin percentageorbydefiningadesiredbackpressurevalue Refe...

Page 52: ...containingthesteppermotors camsandactuatingpistons Anelectricalswitchinsidethevalveblockindicatesifavalveisopenorclosed No powerisrequiredtokeepthevalveintheopenorclosedposition Underneaththevalvebloc...

Page 53: ...mnfittingsand connectingtheinletandoutlettubing Changetubing Whenchangingtubingandconnectors followtherecommendationsintheProduct Documentationpackageforappropriatelengths Themanifold Reagents solvent...

Page 54: ...esystemcabinetthereisacoolerexhaust Thecoolerissettokeepthe temperaturebelow40 Cinsidethecabinet Theoutcomingairishot Toavoidheating theprocessroom thisexhaustcanbeconnectedtotheventilationsystem elep...

Page 55: ...mentareroutedthroughthejunctionbox Thepowerandsignalcablesbetweenthe junctionboxandtheprocesssystemarebundledandprotected Allcablesare connectedbyquickconnections sothatthesystemandjunctionboxcanbese...

Page 56: ...ter 5 Powersupplycabletojunctionboxfromfixedpowersupply Aneasy accesscircuit breakermustbeavailable 6 Communicationcabletocomputer 7 Externalsignalcable 8 PowersupplycableviaMAINSSWITCHonjunctionbox 9...

Page 57: ...ting Systembuzzer response UNICORNdisplayresponse Enabled default setting Alarm NoAirSupply Disabled Noalarm NoAirSupply Note In the event that the ethernet connectivity with the system is lost prior...

Page 58: ...gandmixing Thegas regulatorsallowfineadjustmentoftheinertgaspressureto0 1to0 2bar Bothregula torsshouldbeadjustedtogiveapproximatelythesamepressure WARNING Donotconnecttheauxiliaryinertgasoutlettoagla...

Page 59: ...3 Systemdescription 3 3 Systemcomponents 3 3 8 Inertgasdeliverysystem OligoPilot400OperatingInstructions28960368AH 59...

Page 60: ...veatmosphere Storetheglassbottlesandothercontainersinthestandundertheprocesscabinet Forsafetyreasonsthemaximumvolumeofeachbottleis5Landthemaximumtotal volumeofthebottlesallowedinthestandis25L Thestand...

Page 61: ...bletobeusedwiththesystemalsoincludesAxiTide 50and AxiTide140 Thecolumnholderatthefrontoftheprocesssystemcanholda150mmcolumn weighingupto15kg Thecolumnholdercanbeswung180 Ctoallowtheuserto emptyandclea...

Page 62: ...ystemflowpath 22 A B XV 001 XV 002 XV 003 19 3 4 XV 022 XV 023 XV 021 20 21 5 2 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 XV 051 4 3 1 XV 052 4 3 2 1 XV 061 1 2 3 4 8 15 6 7 17 16 18 9 10 11 12...

Page 63: ...etonitrile 8 Detritylationsolution 9 Oxidationreagent 10 Activator 11 Extra1 12 Thiolationreagent 13 CappingA 14 CappingB 15 Stop 16 Stop 17 Stop 18 Waste W1 19 PressuresensorPT 202 20 PumpP 202 21 Pr...

Page 64: ...sunderMicrosoft Windows operatingsystem UNICORN issupplied withanumberofready mademethodtemplatesthatprovidecreationofmethodsfor synthesis FormoreinformationaboutUNICORNcontrolsystem seetheUNICORN use...

Page 65: ...onsinthe Safetyinstructionschapterregardingflammableliquidsandexplo siveenvironmentbeforeproceedingwithanyactionsorproce duresdescribedinthischapter WARNING Beforeattemptingtoperformanyoftheprocedures...

Page 66: ...Explosionhazard Theproductusespotentiallyexplosiveliquids Consultlocalauthoritiesregardinglocalrulesandregulations beforeinstallingoroperating WARNING Afumehoodorsimilarventilationsystemshallbeinstall...

Page 67: ...ysbe easytodisconnect WARNING Alwaysdisconnectpowerfromthe OligoPilot400 instrument beforeaninstrumentmoduleisremovedorinstalled oracableis connectedordisconnected NOTICE Donottouchthetipsoftheoptical...

Page 68: ...ning temperature is 18 22 C for the maximum lifespan of the components and the optimal chemistry conditions Placement Wellventilatedareainthelab 0 5m s Humidity 0 to70 non condensing PneumaticInstru m...

Page 69: ...pressedair Inertgas Power Reagents Columnwithsolidsupport Makesurethat UNICORN isinstalledonthecomputer ForadescriptionofpowersupplyandcommunicationconnectionsrefertoIllustration on page 56 Transport...

Page 70: ...ping andcolumnareconnectedtotheplantgroundingnetwork Usingonlynon sparkingtoolsratedforexplosiveatmospheres Alwaysusingantistaticcleaningcloths WARNING Atleasttwopersonsarerequiredformovingthesystem N...

Page 71: ...Raisethecabinet Raisethecabinetwhenthesystemisinpositioninsidethefumehood Followthe instructionbelowtoraisethecabinet 4 Installation 4 2 Setup OligoPilot400OperatingInstructions28960368AH 71...

Page 72: ...fore lowering the cabinet always make sure that no bottles are placed on the stand under the cabinet Powersupplyandcommunication WARNING Beforeconnectingthepowercordmakesurethatthesupply voltageatthew...

Page 73: ...systemStrategyfrom UNICORN 7 Note Enabled antivirus programs can cause the communication with OligoPilot 400 to fail Sometimes re installation of UNICORN might even be necessary if the antivirus progr...

Page 74: ...inletandoutletconnectionsontheprocesssystem seeProduct Documentationfortubingdrawings Checkthattheinletandoutlettubingdimensionsmeetthespecification see Product Documentation Flushingandfillingthesyst...

Page 75: ...inletpressuretothesystemishigherthan normal ThePCV 341settingsareoptimizedforafeedinletpressureof0 3bar Toadjustthezeroflowset pointlowertheMinPossettinginstepsof5 untiltheflow iscompletelystopped Ifr...

Page 76: ...ertgaspressureinthebottlecreateaflow 3 SlowlyclosethePCV 341valveusingManPCV 341settings Result Whentheflowstops youknowwhatthe0 opensettingshouldbe 4 StartahighflowratewiththePCV 341valve100 openands...

Page 77: ...sive environment on page 13 Personal protection on page 14 System operation on page 18 WARNING Beforeoperation allprocessconnectionsandthepipingsystem mustbetestedforleakageatmaximumpressureforcontinu...

Page 78: ...andthefeedtankregularly NOTICE Waterwillseriouslydamagetheresultofanoligonucleotide synthesis NOTICE Ifthepressureistoolowinthebottlesitmaycausecavitation NOTICE Tetrahydrofuran THF isnotcompatiblewit...

Page 79: ...toluenemaynotbeused WARNING Whenusinghazardouschemicals allsuitableprotectivemeasures suchaswearingprotectiveglassesandacetonitrile resistant gloves mustbetaken NOTICE Oligonucleotidesynthesisrequires...

Page 80: ...bottle Prerequisites Before OligoPilot400 istakenintooperation makesurethatallrelevantproceduresin Chapter 4 Installation on page 65havebeenperformed Safetydistanceforsourcesof ignition Itisimportantt...

Page 81: ...penedandwastetubing isconnectedtoawastecontainer 3 Turnonthepowerto OligoPilot400 byturningtheMAINSSWITCH located onthejunctionbox toposition1 Thepre purgingprocedureoftheprocesscabinetstarts Whensucc...

Page 82: ...nthe UNICORN Logondialogappears selectauserfromtheUserslist andenterthepassword Ifyoulogonfortheveryfirsttime selectdefaultandenterthepassword default 4 ClickOK 5 IntheSystemControlmodule selectSystem...

Page 83: ...olecularsieves notinBeaucagereagent Inertgassupply Checkthattheinertgassupplyisfreefromwaterandcarbondioxide bothofwhichcan seriouslyreducethesynthesisefficiency Whenchangingtheinertgascylinder make s...

Page 84: ...dAxiTide140aremanufacturedtocustomorderandhaveindividual productcodes Contactyourlocal Cytiva representativeforfurtherinformation 5 Operation 5 1 Preparethesystem 84 OligoPilot400OperatingInstructions...

Page 85: ...non sparkingtoolsratedforexplosiveatmospheres Alwaysusingantistaticcleaningcloths WARNING Explosionhazard Makesurethatthepowertotheprocess systemisturnedoffwhenpackingandconnectingthecolumn WARNING Ex...

Page 86: ...ryinapproximately500mL FineLINE 70 or1000mL FineLINE100 ofacetonitrile 4 Fromtheamountofslurry calculatetherequiredbedheight Bed height gram support 0 111 gram mL radius2 Note Primer Support 5G 111 mg...

Page 87: ...cefromthetopofthepiston equaltothebedheight a a 8 Lockthecolumnverticallyinthe OligoPilot400 columnholder 9 Connecttheearthcablebetweenthecolumnandthesystemhousing 5 Operation 5 2 Columnpackingprocedu...

Page 88: ...out2to3cmfromthecolumntubeupperedge just belowwherethecolumntubewidens 4 Connectoneendofa2mtubingtotheadapter columninlet 1 andthe otherendtothebottlecaponanempty2literbottle Connectaventilation tubet...

Page 89: ...e Ifthisdoesnot happen applyalittlemanualdownwardpressureontheadapter 5 Whenthemarkhasreachedthetoplid openthehydraulicoutletballvalve 4 completelyandswitchofftheflowrate 6 Closethehydraulicoutlet 4 7...

Page 90: ...orinconnectionstoit Beforestartingaruncheckthat Themanualcolumninletandoutletvalvesareopen Thecolumnisproperlyconnectedbetweenthetwocolumnvalves Itshouldbe connectedtoport3oneachvalve Port1isthebypass...

Page 91: ...d variables The main purpose is to prevent siphoning of solvents in the column and to create a 1 to 3 bar backpressure during the coupling step Performingarun Seethe UNICORN usermanualsforthefollowing...

Page 92: ...Section Seepage 5 4 1 Unpackthecolumn 93 5 4 2 Proceduresafterarun 96 Precautions NOTICE Avoidcondensationbylettingtheunitequilibratetoambient temperature 5 Operation 5 4 Proceduresafterarun 92 Oligo...

Page 93: ...on 3 6 Flowchart on page 62forlocationof thetubing 4 Connecttubingfromcolumnoutlet 2 toa1literwastebottlewithabottle cap Note Make sure that the tubing is securely tightened to the bottle cap and that...

Page 94: ...inletvalve 3 Makesurethatthereisenoughfreespaceabovethecolumnfortheadapter tomoveuptotheuppermostposition 12 Apply2to3barinertgaspressure Theadaptermovesupwardsuntilit reachesthewiderpartofthecolumn 1...

Page 95: ...andwillcomeoutafterafewseconds the puckmethod Note Hold the beaker securely since the support will come out with high speed from the column 5 Stoptheinertgaswhenunpackingisfinished 5 Operation 5 4 Pro...

Page 96: ...rotectionandpostdeprotection Fordetaileddescriptionofdeprotectionreferto Cytiva user manuals andapplication notesforoligonucleotidesynthesis Forpostdeprotection washthesupportwith1CVethanol waterfollo...

Page 97: ...epresentativeformoreserviceinformation Inthischapter Section Seepage 6 1 Usermaintenanceschedule 101 6 2 Componentmaintenance 103 6 3 Cleaning 118 6 4 Storage 119 Precautions WARNING Beforeattemptingt...

Page 98: ...WARNING Explosionhazard Onlyuseinertgasforflushingsolventsoutof thesystem andbeforefillingitwithsolvents Otherwise airmight entertheflowpathandcreateanexplosiveatmospherewhen gettingmixingwithsolvent...

Page 99: ...rtgaspressureinthebottlesbeforeattemptingto replaceanyitemonthesystem WARNING Donotperformanytypeofmaintenanceworkwhilethesystemis poweredelectricallyorwhenthepipingsystemispressurized Note thatthepip...

Page 100: ...ageat maximumpressureforcontinuedprotectionagainstinjuryrisks duetofluidjets burstpipesorexplosiveatmosphere WARNING Forcontinuedprotectionfromfirehazard replaceonlywithsame typeandratingoffuse 6 Main...

Page 101: ...Ifthereissignofliquidleakingbetweenapumphead andthesystempanel orincreasedordecreased volumeofrinsingsolution contact Cytiva represen tativetoreplacethepistonassembly ceramictube and orO ringsinthepum...

Page 102: ...ringsand sealings Thereplacementshouldbeperformedbypersonnel authorizedby Cytiva Valves Replacethemembranes SeeSection 6 2 2 Membrane valve block on page 113 andSection 6 2 3 PCV valve on page 117 Thi...

Page 103: ...provedorsuppliedby Cytiva maybeusedformaintainingorservicing OligoPilot400 Tubingandconnectors NOTICE Alwaysusenewtubingandferrules Tubingandferrulesthathave beenusedordisconnectedshouldnotbere used S...

Page 104: ...necessary Ifadamagedpistonhasbeeninoperation theceramictubemightbedamagedaswell andshouldalsobereplaced Ifcleaningofafaultycheckvalvedoesnotimproveitsperformance itshouldbe replaced Note Do not disass...

Page 105: ...chamber 6 Ceramictube 7 O ring thin 8 Rinsechamber 9 Pistonrod 10 Returnspring 11 Pistonassembly 400mL 12 Adapter Torque3to3 5Nm 13 Checkvalveinner rinsingsystem Torque3to3 5Nm 14 Piston 15 Screw 2pcs...

Page 106: ...onotremovethemcompletely 5 Usingthehexkey unscrewthetwoscrewslockingthepumpheadinposi tion Loosenthescrewscrosswise halfaturnatatime whilepushingfirmly onthefrontfaceofthepumpheadtocompensateforthepre...

Page 107: ...ged thepistonassemblyshouldbereplaced 11 Wipethepistonwithacleancloth Inspectthepistonwithamagnifyingglass forscratches Replacethepistonassemblyifanyscratchesorcracksarefound 12 Scrapeoffanydepositswi...

Page 108: ...hthetriangularhole 2 Inspectthenutsandthetubesfordirtordamage 3 Replacewithanewcheckvalveifanydirtordamageisfoundandcleaning doesnotimprovetheperformanceoftheoldcheckvalve Tightenthenewcheckvalveusing...

Page 109: ...head Step Action 1 MakesurethatthenewO ringsareinstalledonthepumpheadfront thick O ring andtherinsechamber thinO ring 2 Withthepumpheadfrontfacingdownwardsonthebench placetheliquid chamberontothepumph...

Page 110: ...Useatorque wrenchtotightenthecomponents Installthepumphead Step Action 1 Turnthepumpheadsothattheinnercheckvalveisfacingupwardsandthe drainageholedownwards Mountthecompletepumpheadoverthelocating pins...

Page 111: ...ouschemicalsare inuse Connectthetubingbyfirstinsertingthetubingfully then tighteningtheconnectorfingertight DonottightenETFE ethylene tetrafluoroethylene tubingfurtherasthiswilldamage theendofthetubin...

Page 112: ...nlettubingtotheinletcheckvalve 3 Connecttherinsingsystemtubing SeeSection 3 6 Flowchart on page 62 4 Thepumpisreadytobeprimed 6 Maintenance 6 2 Componentmaintenance 6 2 1 Pumps 112 OligoPilot400Operat...

Page 113: ...ntconnectionblockshaveseparatecode numbers refertotheProductDocumentationpackagedeliveredwiththeproduct Valvemembranekitcontaining4membranes 7mmwrench Twopersonsarerequiredtoreplacethewettedpartsofthe...

Page 114: ...nectalltubingfromthevalve 5 Removethesixattachmentscrewsusingthewrench 6 Carefullyloosentheoldconnectionblock 7 Pulloutanddiscardtheoldmembranes Note Be careful not to scratch the mechanical housing o...

Page 115: ...fore insertion 2 Lockthepositionofthemembranesinthevalveseatbypressingthe connectionblockagainstthemembranes 3 EnableSetValvePositionunderSettings Specials 4 SetthevalvetoopenunderSetting Special SetV...

Page 116: ...ion 7 DisableSetValvePositionunderSettings Specials Thevalveswillreturn totheirnormalpositions 6 Maintenance 6 2 Componentmaintenance 6 2 2 Membranevalveblock 116 OligoPilot400OperatingInstructions289...

Page 117: ...hebackpressureisfoundtobehaveabnormallyduringtheoperation i e notaccordingtothebackpressurespecifiedforthePCV 341 oneofthefollowing problemshasprobablyoccurred IPconvertercontrollingthePCV 341ismalfun...

Page 118: ...lationinletlinesshouldbewashedwithacetonitrileaftereachruntoavoidtherisk ofprecipitationinthelines Cleaningbeforeplanned maintenance service Toensuretheprotectionandsafetyofservicepersonnel allequipme...

Page 119: ...tesolutionsat4 Candactivatorsolutionatroomtempera ture Note Some activators might crystallize at temperatures below 18 C 4 Connectacetonitrilebottlestotheamidite activatorandthesolventlines Note Check...

Page 120: ...4 Power supply on page 17 Maintenance on page 20 WARNING Explosionhazard Avoiddischargefromstaticelectricitywhen workinginpotentiallyexplosiveatmospheresby Alwaysusingsuitableclothing Makingsurethatth...

Page 121: ...nthecolumn frompreviousruns Cleanthecolumnaccordingtothecolumninstruc tions NoisyUV signal signal driftorinsta bility BadUVfiberconnec tions ChecktheconnectionsoftheUVcellopticalfiber Replaceifnecessa...

Page 122: ...actoris inuse SeeMonitor pH C 900 User Manual Pressurecurve Error symptom Problem Correctiveaction Erraticflow noisybaseline signal irregular pressuretrace Airbubblespassing throughortrappedinthe pump...

Page 123: ...Leakingconnection and orcrystallizedmate rialaroundthetubing connector Unscrewtheconnectorandcheckifitiswornor incorrectlyfitted Ifrequired replacetheconnector Tightentheconnectorwithyourfingersonly E...

Page 124: ...ththe valvebody fromthedrain holes Thevalvemembrane mightbewornor damaged Changethevalvemembrane SeeSection 6 2 2 Membrane valve block on page 113 Highbackpres sure Dirtintheflowpath Cleanthevalvebyfl...

Page 125: ...ushingwithacetoni trile Purgesystem Error symptom Problem Correctiveaction Thespecified pressureinthe cabinetcannot bemaintained Thereardoorisnot closed Closethedoor Thecompressedair supplyisnotsuffic...

Page 126: ...maintainingandtroubleshooting OligoPilot400 Inthischapter Section Seepage 8 1 Specifications 127 8 2 Chemicalresistance 129 8 3 Recommendedreagents 133 8 4 Recyclinginformation 134 8 5 Regulatoryinfo...

Page 127: ...roperation 18 Cto22 C Note The recommended ambient tempera ture is for the maximum lifespan of the components and the optimal chemistry conditions Wettedmaterialsdata Thefollowingcomponentsin OligoPil...

Page 128: ...sensor AE 131 UVcell2mm Lenshousing O ring5x1 35 Housing Lens P 201A P 201B Pumphead PumpHead CeramicTube O ring Sealing Valvehousing Ballstopper Rubyball Lowerlid PT 201 PT 202 Pressuresensor FT 141...

Page 129: ...hyde OK OK Aceticacid 5 OK OK Aceticacid 70 OK OK 64 19 7 200 580 7 Acetonitrile OK OK 75 05 8 200 835 2 PPandPEswell Acetone 10 OK Avoid PVDFisaffected bylongtermuse Ammonia 30 OK OK 7664 41 7 231 6...

Page 130: ...OK Avoid Siliconenot resistant Pres surelimitfor PEEKdecreases Ethyleneglycol 100 OK OK 107 21 1 203 47 3 3 Formicacid 100 OK OK 64 18 6 200 579 1 Siliconenot resistant Glycerol 100 OK OK 56 81 5 200...

Page 131: ...glassareaffected bylongtermuse Potassium carbonate OK OK 584 08 7 209 52 9 3 Potassium chloride OK OK 7447 40 7 231 2 11 8 Pyridine OK OK Sodiumacetate OK OK Sodiumbicar bonate OK OK Sodiumbisul phate...

Page 132: ...void Avoid Silicone PPand PEarenot resistant Tetrahydrofuran Avoid Avoid ETFE CTFE PP andPEarenot resistant Toluene OK OK Trichloroacetic acid 1 OK OK 76 03 9 200 927 2 Trifluoroacetic acid 1 OK OK 17...

Page 133: ...tsService CAS numbers Chemical CASNo Diethylamine 109 89 7 Acetonitrile 75 05 8 Iodine 7553 56 2 Pyridine 110 86 1 Water 7732 18 5 1 Methylimidazole 616 47 7 Aceticanhydride 108 24 7 Lutidin 108 48 5...

Page 134: ...thedifferentmaterialsmustbeseparatedand recycledaccordingtonationalandlocalenvironmentalregulations Recyclingofhazardoussubstances Theproductcontainshazardoussubstances Detailedinformationisavailable...

Page 135: ...opeanUnionandEuropeanEconomicArea 137 8 5 3 EurasianEconomicUnion 138 8 5 4 RegulationsforNorthAmerica 140 8 5 5 Regulatorystatements 141 8 5 6 DeclarationofHazardousSubstances DoHS 142 8 5 7 Notifica...

Page 136: ...Manufacturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB Bj rkgatan30 SE75184Uppsala Sweden Telephonenumb...

Page 137: ...notincludedwiththeproduct acopyoftheEUDeclarationofConformityisavailable onrequest CEmarking TheCEmarkingandthecorrespondingEUDeclarationofConformityisvalidforthe instrumentwhenitis usedaccordingtothe...

Page 138: ...ingtableprovidessummaryinformationaboutthemanufacturerand importer inaccordancewiththerequirementsoftheTechnicalRegulationsofthe CustomsUnionand or theEurasianEconomicUnion Requirement Information Nam...

Page 139: ...thesystem label ThisEurasiancompliancemarkindicatesthattheproductis approvedforuseonthemarketsoftheMemberStatesofthe CustomsUnionoftheEurasianEconomicUnion 8 Referenceinformation 8 5 Regulatoryinforma...

Page 140: ...topart15oftheFCCRules Theselimitsaredesignedtoprovide reasonableprotectionagainstharmfulinterferencewhentheequipmentisoperatedin acommercialenvironment Thisequipmentgenerates uses andcanradiateradio f...

Page 141: ...ts EMCemission CISPR11 Group1 ClassAstatement NOTICE Thisequipmentisnotintendedforuseinresidentialenvironments andmaynotprovideadequateprotectiontoradioreceptionin suchenvironments 8 Referenceinformat...

Page 142: ...leakormutateundernormal operatingconditionssothattheuseofsuchelectricalandelectronicproductswillnotresultin anysevereenvironmentalpollution anybodilyinjuryordamagetoanyassets Theunitofthe periodis Yea...

Page 143: ...edinallofthehomogeneous materialsforthispartisbelowthelimitrequirementinGB T26572 X Indicatesthatthishazardoussubstancecontainedinatleastoneofthe homogeneousmaterialsusedforthispartisabovethelimitrequ...

Page 144: ...alandrecycling pleasereferto https www ec gc ca mercuremercury Default asp lang En n DB6D2996 1 Ifadditionalsupportisneeded pleasecontactyourCytivarepresentative Thisproductshouldbedisposedoforrecycle...

Page 145: ...BiologicalReactivityTests InVivo EMA 410 01 Guidanceonminimisingtheriskoftransmitting animalspongiformencephalopathyagentsviahuman andveterinarymedicinalproducts Animalorigin freematerial 21CFR177 Ind...

Page 146: ...ME BPE GMPandcGMPsinformation refertotheproduct specificspecificationinformationprovidedinthedocumentation packagedeliveredwitheachequipment 8 Referenceinformation 8 5 Regulatoryinformation 8 5 8 Othe...

Page 147: ...r instrument service repair or installation In some cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have be...

Page 148: ...must be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equi...

Page 149: ...ackage 8 software 9 E Emergency 27 28 Procedures 27 Shutdown 28 Stop 28 Emergencystop 38 39 F FCCcompliance 140 Flowchart 62 G Glassbottle 60 I Importantuserinformation 5 Inertgas 58 83 J Junctionbox...

Page 150: ...tion 134 decontamination 134 disposalofelectricalcompo nents 134 recyclingofhazardous substances 134 Referenceinformation 126 Regulatoryinformation 135 S Safetylabels 26 Safetynotices 6 Safetyprecauti...

Page 151: ...Pageintentionallyleftblank...

Page 152: ...igonucleotidesonsolidsupportsiscoveredbyUSpatent number6 887 990andequivalentpatentsandpatentapplicationsinothercountries andalicensetheretoisonlygivenwhen synthesisofoligonucleotidesisperformedonCyti...

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