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5.6

Autozero

The autozero function sets the detected absorbance to zero when the 

autozero key is

pressed. Autozero is recommended before a sample is injected.
Press the 

autozero key. The absorbance value is then shown in the main operating

menu.

5 Operation

5.6 Autozero

50

 Monitor UVis-920 Operating Instructions 29055049 AF

Summary of Contents for Monitor UVis-920

Page 1: ...Monitor UVis 920 Operating Instructions Original instructions Translation disc included cytiva com ...

Page 2: ...Pageintentionallyleftblank ...

Page 3: ...ll2mmandUVFlowCell10mm 31 4 5 2 UVFlowCellÄKTApilot1 2 5mmandIndustrialFlowCell8mm and1inch 37 4 6 Electricalconnections 39 5 Operation 42 5 1 Menuselection 43 5 2 Startingtheinstrument 46 5 3 Mainmenuoverview 47 5 4 Settingwavelength 48 5 5 Customfilters 49 5 6 Autozero 50 5 7 Readingabsorbancevalues 51 5 8 Setupmenu 52 5 9 Checkmenu 57 5 10 UVcellcalibration 60 5 11 Changingflowcell 61 6 Mainten...

Page 4: ... 80 8 3 2 EuropeanUnionandEuropeanEconomicArea 81 8 3 3 EurasianEconomicUnion Евразийскийэкономическийсоюз 82 8 3 4 RegulationsforNorthAmerica 84 8 3 5 Regulatorystatements 85 8 3 6 DeclarationofHazardousSubstances DoHS 86 8 4 Orderinginformation 88 8 5 HealthandSafetyDeclarationForm 91 TableofContents 4 MonitorUVis 920OperatingInstructions29055049AF ...

Page 5: ...fsafetynotices regu latoryinformation intendeduseoftheMonitorUVis 920instrument andlistsofasso ciateddocumentation Inthischapter Section Seepage 1 1 Aboutthismanual 6 1 2 Importantuserinformation 7 1 3 Associateddocumentation 9 1 Introduction MonitorUVis 920OperatingInstructions29055049AF 5 ...

Page 6: ...ll operate andmaintaintheproductinasafeway Typographicalconventions Softwareitemsareidentifiedinthetextbybolditalictext Hardwareitemsareidentifiedinthetextbyboldtext Inelectronicformat referencesinitalicsareclickablehyperlinks 1 Introduction 1 1 Aboutthismanual 6 MonitorUVis 920OperatingInstructions29055049AF ...

Page 7: ...dures orfordiagnosticpurposes Prerequisites InordertooperateMonitorUVis 920inthewayitisintended Theusermustbeacquaintedwiththeuseofbioprocessingequipmentandwiththe handlingofbiologicalmaterials TheusermustreadandunderstandtheSafety instructionschapterintheOperating Instructions ThesystemmustbeinstalledaccordingtotheinstructionsintheInstallation chapteroftheOperating Instructions Safetynotices This...

Page 8: ...TICEindicatesinstructionsthatmustbefollowedtoavoid damagetotheproductorotherequipment Notesandtips Note A note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your proce dures 1 Introduction 1 2 Importantuserinformation 8 MonitorUVis 920OperatingInstructions29055049AF ...

Page 9: ... containingdetailedspecificationsandtraceabilitydocuments Themostimportantdocumentsinthedocumentationpackage withregardtotech nicalaspectsofMonitorUVis 920 arelistedinthetablebelow Documentation Maincontents Monitor UVis 920 Operating Instruc tions Instructionsneededtoinstall operate andmaintainMonitorUVis 920inasafe way DeclarationofConformity DeclarationofConformityforEUand or otherregions 1 Int...

Page 10: ...lsthatareattachedtothe equipment Inaddition thechapterdescribesemergencyandrecoveryprocedures andprovidesrecyclinginformation Inthischapter Section Seepage 2 1 Safetyprecautions 11 2 2 Labels 15 2 3 Emergencyprocedures 16 2 Safetyinstructions 10 MonitorUVis 920OperatingInstructions29055049AF ...

Page 11: ...pmentinthearea Thesafetyprecautionsinthissectionaregroupedintothefollowingcategories Generalprecautions Personalprotection Installingandmovingtheproduct Operation Maintenance Generalprecautions WARNING Donotoperatetheproductinanyotherwaythandescribedinthe userdocumentation WARNING Onlyproperlytrainedpersonnelmayoperateandmaintainthe product Personalprotection WARNING AlwaysuseappropriatePersonalPr...

Page 12: ...thenationalcodeofpracticefor biosafety Installingandmovingtheproduct WARNING HighintensityUVlight Thisproductuseshighintensityultra violetlight Donotdisconnecttheopticalfiberswhilethelampis on WARNING Protectiveground Theproductmustalwaysbeconnectedtoa groundedpoweroutlet WARNING Powercord Onlyusepowercordswithapprovedplugsdelivered orapprovedbyCytiva WARNING Accesstopowerswitchandpowercordwithplu...

Page 13: ...insideandtheoutsidebeforereconnecting thepowersupply Maintenance WARNING Electricalshockhazard Allrepairsshouldbedonebyservice personnelauthorizedbyCytiva Donotopenanycoversorreplace partsunlessspecificallystatedintheuserdocumentation WARNING Disconnectpower Alwaysdisconnectpowerfromtheinstru mentbeforeperforminganymaintenancetask WARNING Hazardouschemicalsorbiologicalagents Whenusing hazardousche...

Page 14: ...alth Whenusinghazardouschemicals avoidspillageand wearprotectiveglassesandothersuitablePersonalProtective Equipment PPE CAUTION HazardouschemicalsorbiologicalagentsinUVflowcell Makesurethattheentireflowcellhasbeenflushedthoroughly withbacteriostaticsolution e g NaOH anddistilledwater before serviceandmaintenance 2 Safetyinstructions 2 1 Safetyprecautions 14 MonitorUVis 920OperatingInstructions2905...

Page 15: ...serdocumentationbeforeusingthesystem Donotopenanycoversorreplacepartsunlessspecificallystated intheuserdocumentation CAN ICES 1 NMB 1 CANICES 1 NMB 1indicatesthatthisproductcomplieswiththe CanadianstandardICES 001concerningtechnicalrequirements relativetoradiatednoiseemissionsfromIndustrial Scientificand Medicalradiofrequencygenerators Codeno Instrumentassemblynumber Serialno Instrumentserialnumbe...

Page 16: ...rtotheinstrumentbyswitchingtheOn offswitchonthe rearpaneltothe O position Result Therunisinterruptedimmediately 2 Disconnectthemainspowercord Powerfailure Intheeventofpowerfailuretherunisinterruptedimmediately Restartafterpowerfailure Ifthepowersupplytotheinstrumentisinterrupted theinstrumentautomatically restartsitselfanddisplaysthemainoperatingmenu Allsetvaluesareretainedinthe instrumentbutthein...

Page 17: ...vesanoverviewoftheMonitorUVis 920instrument andabriefdescrip tionofitsfunction Inthischapter Section Seepage 3 1 Instrumentoverview 18 3 2 Monitorprinciple 21 3 3 Flowcellprinciple 23 3 Instrumentdescription MonitorUVis 920OperatingInstructions29055049AF 17 ...

Page 18: ...angebetween200 700nmbychangeablefixedwavelengthfilters The instrumentcontainsnointernaluserreplaceableitems Illustrationoftheinstrument TheillustrationbelowshowstheMonitorUVis 920instrument 1 2 Part Function 1 Mainunit 2 UVcellholderwithcover 3 Instrumentdescription 3 1 Instrumentoverview 18 MonitorUVis 920OperatingInstructions29055049AF ...

Page 19: ... 920 1 2 3 4 5 6 7 8 9 10 Part Function 1 Ejectbuttonforfilterunit 2 OKkey 3 run endkey 4 Display 5 Arrowupkey 6 Arrowdownkey 7 eventmarkkey 8 autozerokey 9 Esckey 10 Filterunitinsert 3 Instrumentdescription 3 1 Instrumentoverview MonitorUVis 920OperatingInstructions29055049AF 19 ...

Page 20: ...quency 50 60 Hz Power max 35 VA 1 2 3 4 5 Part Function 1 Analogout0 1V Recorderoutput 1channel0Vto1V 2 Forserviceuseonly 3 Remote Digitalcontrolsignals 4 Instrumenton offswitch 5 Mains Supplyvoltage grounded 3 Instrumentdescription 3 1 Instrumentoverview 20 MonitorUVis 920OperatingInstructions29055049AF ...

Page 21: ...romatortotheflowcellandfromthecelltothedetectorelectronicsisguided byopticalfiberswhichfocusitsfullintensityontheliquidflowpath maximizingthe sensitivityofthemonitoring Beforeenteringtheflowcell themonochromaticlightis splitinabeamsplitter with50 ofthelightpassingthroughthesamplefiber S and theflowcell and50 beingdirectedthroughthereferencefiber R Twophotodiodes withidenticalcharacteristicsmonitor...

Page 22: ...t Function 4 Interferencefiltermonochromator 5 Reference 6 Microcontroller 7 Signal 8 Opticalfibres 9 Flowcell 3 Instrumentdescription 3 2 Monitorprinciple 22 MonitorUVis 920OperatingInstructions29055049AF ...

Page 23: ... Thedesignoftheflowcellabovepreventstheformationofdistinctinterfacesbetween eluentcomponentswithdifferentrefractiveindicesandeliminatesthenegativeinflu encethesewouldcause Theprecisionofmonitoringisenhancedbytheconstruction oftheflowcell whichensurestotalreflectanceoflight Thismaintainsahighintensity oflighttothedetector Thelongpathlengthcombinedwithasmallcellvolume increasessensitivity 3 Instrume...

Page 24: ...G Beforeattemptingtoperformanyoftheproceduresdescribedin thischapter youmustreadandunderstandallcontentsofthe Safetyinstructionschapter Inthischapter Section Seepage 4 1 Siterequirements 25 4 2 Unpacking 26 4 3 Transport 27 4 4 Installingthecellholder 28 4 5 Installingtheflowcells 30 4 6 Electricalconnections 39 4 Installation 24 MonitorUVis 920OperatingInstructions29055049AF ...

Page 25: ...ces draughtsanddirectsunlight Toensurecorrectventilationafreespaceof0 1misrequiredbehindandinfrontofthe instrument Donotuseanysoftmaterialundertheinstrument toensurethatthe ventilationinletinthefrontisnotblocked Theinstrumentshouldnotbeusedinacorrosiveatmosphereorinanatmosphere wheredepositsareliabletoformontheopticalsurfaces Electricalpowerrequirements Parameter Requirement Supplyvoltage 100 240V...

Page 26: ...arenoloosepartsinthetransportcrate Checkthatallthepartsareeithermountedonthesystemorlocatedintheacces sorykitbox Ifanydamageisfound documentthedamage andcontactyourlocalCytivarepresen tative Itisrecommendedthatallpackingmaterialsshouldberetainedifonwardtransportof theinstrumentisexpected 4 Installation 4 2 Unpacking 26 MonitorUVis 920OperatingInstructions29055049AF ...

Page 27: ...4 3 Transport Beforemovingtheequipment disconnectallconnectedcablesandtubing 4 Installation 4 3 Transport MonitorUVis 920OperatingInstructions29055049AF 27 ...

Page 28: ...ctuseshighintensityultra violetlight Donotdisconnecttheopticalfiberswhilethelampis on NOTICE Toavoiddamagingtheopticalinletandoutletthetwoblackrubber capsattheopticalfiberconnectorsmustbeinplacewheninstal lingthecellholder Step Action 1 Unpackthecellholder 2 SlidethecellholderinplaceontherighthandsideoftheMonitorUVis 920 instrument 4 Installation 4 4 Installingthecellholder 28 MonitorUVis 920Opera...

Page 29: ...Step Action 3 Fastentheflowcellholderwiththetwoscrewsincluded Useahexwrench 4 Installation 4 4 Installingthecellholder MonitorUVis 920OperatingInstructions29055049AF 29 ...

Page 30: ...UVFlowCell10mm UVFlowCellÄKTApilot 1 2 5mm IndustrialFlowCell8mm tubingi d 8mm IndustrialFlowCell1inch tubingi d 1inch Inthissection Section Seepage 4 5 1 UVFlowCell2mmandUVFlowCell10mm 31 4 5 2 UVFlowCellÄKTApilot1 2 5mmandIndustrialFlowCell 8mmand1inch 37 4 Installation 4 5 Installingtheflowcells 30 MonitorUVis 920OperatingInstructions29055049AF ...

Page 31: ...ectivecapsontheopticalfiberconnectors 2 Slidetherearwhitecliponthecellholdertoitsinnerpositionfora2mmcell andtoitsouterpositionfora10mmcell 3 Placetheflowcellintheopeningbetweenthewhiteclips Toimprovethe accesstothetubingconnectionsthecellcanbepositionedwiththetextand serialnumberfacingupwardsorsideways Pressthecellintotheclipsto fastenit Normalposition Theserialnumberisfacingupwards 4 Installatio...

Page 32: ... Theserialnumberisfacingsideways Connectingtheopticalfibers Followthestepsbelowtoinstalltheopticalfibers 4 Installation 4 5 Installingtheflowcells 4 5 1 UVFlowCell2mmandUVFlowCell10mm 32 MonitorUVis 920OperatingInstructions29055049AF ...

Page 33: ...NOTICE Donottouchthetipsoftheopticalfiberwithanythingotherthan lenspaper NOTICE Donottwisttheopticalfibersduringtightening Step Action 1 Removethetwoblackprotectivecapsfromtheopticalfiberconnectors 4 Installation 4 5 Installingtheflowcells 4 5 1 UVFlowCell2mmandUVFlowCell10mm MonitorUVis 920OperatingInstructions29055049AF 33 ...

Page 34: ...thefiberdetach menttoolsupplied Donotovertighten Fiberdetachmenttool Note The optical fiber end with black shrinking tubing shall be connected to the rear socket connector on the side of Monitor UVis 920 4 Slidetherubbersleevesonthetwoopticalfibersontotheconnectors Make surethatthesleevesarepushedtighttothehousingtopreventdust fluidor lightenteringtheconnection 4 Installation 4 5 Installingtheflow...

Page 35: ...doutletoftheflowcelland connectthetubingwith1 16inchfingertightconnectors 6 Mountthecellholdercoverbypushingitontothecellholder 4 Installation 4 5 Installingtheflowcells 4 5 1 UVFlowCell2mmandUVFlowCell10mm MonitorUVis 920OperatingInstructions29055049AF 35 ...

Page 36: ...smalllugsonthecoverintheholesatthefrontandrearofthecell holder 8 Lowerthecoveroverthecellholder 4 Installation 4 5 Installingtheflowcells 4 5 1 UVFlowCell2mmandUVFlowCell10mm 36 MonitorUVis 920OperatingInstructions29055049AF ...

Page 37: ...achtheflowcelltoanappropriateflowcellholder 4 Connecttheinletandoutlettubingtotheflowcell Connectingtheopticalfibers Followthestepsbelowtoinstalltheopticalfibers WARNING HighintensityUVlight Thisproductuseshighintensityultra violetlight Donotdisconnecttheopticalfiberswhilethelampis on NOTICE Donottouchthetipsoftheopticalfiberwithanythingotherthan lenspaper NOTICE Toavoiddamagingtheopticalfibers pr...

Page 38: ...hesocket andtighteningthenutfingertight fitsonlyoneofthetwosockets 5 AttachtheotherconnectortothetransmitterconnectorofMonitor UVis 920 Donotovertighten Note The optical fiber with the gold colored knurled nut is the transmitter fiber and must not be mixed up with the other fiber the receiver fiber 6 Connecttheotheropticalfibertotheemptysocketontheflowcellandto therearfiberconnectoronthemonitor 4 ...

Page 39: ...edtoa groundedpoweroutlet WARNING Powercord Onlyusepowercordswithapprovedplugsdelivered orapprovedbyCytiva WARNING Accesstopowerswitchandpowercordwithplug Donot blockaccesstothepowerswitchandpowercord Thepowerswitch mustalwaysbeeasytoaccess Thepowercordwithplugmust alwaysbeeasytodisconnect Analogoutconnector 1 2 3 4 5 6 4 Installation 4 6 Electricalconnections MonitorUVis 920OperatingInstructions2...

Page 40: ...e the protective covers from unused connections as a short circuit may disturb the measurements Set the recorder to 0 V to 1 V input full scale 0 V offset Connectingtoauxiliaryequipment if used NOTICE Anycomputerusedwiththeequipmentmustcomplywith IEC 60950orIEC 62368 1andbeinstalledandusedaccordingto themanufacturer sinstructions Connectanyauxiliaryequipmenttothe9 poleD SubfemaleRemoteconnector 5V...

Page 41: ...edgeselectsRun mode starts PositiveedgeselectsEnd mode stops 9 EventMark In Negativeedgemakesaneventmarkon recorder Connectingtosupplyvoltage Followthestepsbelowtoconnecttosupplyvoltage Step Action 1 Makesuretheon offswitchisintheOFFposition O 2 Connectamainscablebetweentheinstrumentandagroundedmains socket TheinstrumentisdeliveredwithbothEuropeanandUStypemains cables asstandard Anyvoltage100Vto24...

Page 42: ...ndunderstandallcontentsofthe Safetyinstructionschapter Inthischapter Section Seepage 5 1 Menuselection 43 5 2 Startingtheinstrument 46 5 3 Mainmenuoverview 47 5 4 Settingwavelength 48 5 5 Customfilters 49 5 6 Autozero 50 5 7 Readingabsorbancevalues 51 5 8 Setupmenu 52 5 9 Checkmenu 57 5 10 UVcellcalibration 60 5 11 Changingflowcell 61 5 Operation 42 MonitorUVis 920OperatingInstructions29055049AF ...

Page 43: ...on page 19 Menunavigation Aspecificmenuisselectedbythefrontarrowdownkey Whentherequiredmenuis visible themenuorselectionisacceptedbypressingtheOKkey Navigatinginsubmenus Ifamenuhassublevels thesubmenuisdisplayedbypressingtheOKkey Pressingthe Esckeymovesbackonemenulevel 5 Operation 5 1 Menuselection MonitorUVis 920OperatingInstructions29055049AF 43 ...

Page 44: ...turnstothemainmenuandthemainoperating window Setup ESC ESC ESC ESC ESC Check Selectvalue Acursorbelowatextornumericalvalueshowswhatisaffectedbyakey Toincrease thevalue pressthearrowupkey Todecreasethevalue pressthearrowdownkey 1 2 3 Part Function 1 Parameter 2 Currentvalue 3 Newvaluetobeset 5 Operation 5 1 Menuselection 44 MonitorUVis 920OperatingInstructions29055049AF ...

Page 45: ...ngarrowdownkeycontinuously Eachcharacterstepsdowntozeroandthenthe cursormovestoleftandstopsatthedesireddecadewhenthekeyisreleased Errormessages Ifanerrorisoccurred thedisplayshowsanerrormessage 1 2 Part Function 1 Errortype 2 Userinformation Tohandleproblemsthroughthedisplayinterfaceevenifanerrormessageisindicated itispossibletoreturntothepreviousdisplay PressOKorEsc Iftheerrorremains a newdisplay...

Page 46: ...einstrumentstartsanddisplaysthemainoperatingwindow Foroptimum performance30minuteswarm uptimeisrecommended AnotherwaytoswitchtheXenon lampon offistosetarunorendtimer SeeSetting run and end timers on page 55 5 Operation 5 2 Startingtheinstrument 46 MonitorUVis 920OperatingInstructions29055049AF ...

Page 47: ...units AU andthemodeofoperation STANDBYorRUNNING Themenuisreachedfromany othermenubypressingEscrepeatedly Setupmenu Setupmenu Setupofanalogout averagingtime serialnumberetc Usethearrow keystoreachthesetupmenu Checkmenu Checkmenu Checkinternaloperatingvalues Usethearrowkeystoreachthecheck menu 5 Operation 5 3 Mainmenuoverview MonitorUVis 920OperatingInstructions29055049AF 47 ...

Page 48: ...adummyfilterisinplaceforprotection Followthestepsbelowtochangethefilterunit Step Action 1 Presstheejectbuttonandremovethefilterunit 2 Insertanewfilterunitinthemonitor Result Theinstrumentreadsthecodetagoftheinsertedfilterunitanddisplayits wavelengthinthemainmenu Emptyfilterholdersforcustomfiltersareavailable Whenusingacustomfilter its wavelengthcanbestoredintheinstrumentbytheoperator SeeSetting wa...

Page 49: ...gwavelengthforcustomfilter Ifacustomfilterisinserted thewavelengthcanbeset Followthestepsbelowtosetthewavelengthforacustomfilter Step Action 1 Insertacustomfilter Result ThedisplayshowsChangeWaveLength 2 SelectyesandpressOK 3 Enterthewavelengthofthecustomfilterinnanometer PressOK Resettingfiltertime Thetimethecurrentfilterhasbeenusedcanbeshowninhoursandresettedtozero Thereisonetimecounterforeachfi...

Page 50: ...tectedabsorbancetozerowhentheautozerokeyis pressed Autozeroisrecommendedbeforeasampleisinjected Presstheautozerokey Theabsorbancevalueisthenshowninthemainoperating menu 5 Operation 5 6 Autozero 50 MonitorUVis 920OperatingInstructions29055049AF ...

Page 51: ...bsorbance values Themainoperatingmenushowstheabsorbancevalues Themenuisreachedfromany othermenubypressingtheEsckeyrepeatedly 5 Operation 5 7 Readingabsorbancevalues MonitorUVis 920OperatingInstructions29055049AF 51 ...

Page 52: ...theabsorptionrangeforrecorderoutputinAUFS pressOK Onlyfixedstepsbetween5 0AUFSand0 001AUFScanbeset 4 SelectmenuSetZeroLevel pressOK Thezeroleveliswhereonthepaper the0AUvaluewillbepositioned Values0 99 5 UsethearrowkeystosetthevalueandpressOK Automaticoverrange Theinstrumenthasanautomaticoverrangefunction Ifthemonitorsignalreachesthe fullscalevalueontherecorder thesignalwillinstantlydropto0Vandgive...

Page 53: ...el absorbanceunits 2 Maximumrange absorbanceunits 3 Overrange 4 Signaltorecorder V Theautomaticoverrangecanbedisabled enabledintheSetupmenu Followthestepsbelowtodisableorenabletheautomaticoverrange Step Action 1 SelectmenuAutomaticOverrange pressOK 2 UsethearrowkeystoselectYesorNo thenpressOK Eventmark 5 Operation 5 8 Setupmenu MonitorUVis 920OperatingInstructions29055049AF 53 ...

Page 54: ...noiseefficiently butitwillalsodistortthepeaks Peaksnarrowerthantheminimumpeakwidthvaluemaybedistorted Becauseofthis theaveragingtimeshouldbeasshortaspossible seethetablebelow Ondeliverythe averagingtimeissetto2 56s Followthestepsbelowtosettheaveragingtime Step Action 1 SelectmenuSetAveragingTime pressOK 2 Usethearrowkeystosetthevalue Usethefixedvaluesbetween5 12 secondsand0 08seconds Averagingtime...

Page 55: ...lowcell PressOK Theserialnumbermustbe1000orhigher 3 SelectmenuSetCellType Checkthecelltypevalue Ifthevalueiscorrect usethearrowkeystogotonextmenu Ifthevaluehastobechanged press OKtochangeit 4 SelectmenuSetCellPathLength pressOK 5 Enterthecalibratedvalueofthecellpathlengthifavailable pressOK The celltypemustalwaysbesetbeforethecalibratedcellpathlength Settingrunandendtimers Followthestepsbelowtoset...

Page 56: ...reshown 3 Setendtime pressOKtoselectaction ChoosetheEndmodetime1 minutesto999minutes Timerisdisabledifsetto0 Iftimerisenabledby OK theremainingminutestorunareshown 4 PressEsckeytoreturntotheRunTimerEndTimermenuwhichnowshows thecountdowntimeandendtime Settingunitnumber Forserviceuseonly 5 Operation 5 8 Setupmenu 56 MonitorUVis 920OperatingInstructions29055049AF ...

Page 57: ...ypeand number Thismenushowsthetypeandserialnumberoftheflowcellstoredinthemonitor Followthestepsbelowtocheckthetypeandserialnumberoftheflowcell Step Action 1 SelectmenuCheck pressOK 2 SelectmenuFlowCell Ifserialnumberisnotset isshown Theserial numbermustbe1000orhigher Checkanalogoutput Thefunctionoftheconnectedchartrecordercanbetested Followthestepsbelowtocheckthefunctionoftheconnectedchartrecorder...

Page 58: ...datthelightdetectors Thesevoltagesare proportionaltothelightintensitiesineachchannel TheR valuegivesanindicationof theconditionofthelamp filterandopticalfibers Followthestepsbelowtocheckthevoltagesgeneratedatthelightdetectors Step Action 1 SelectmenuCheck pressOK 2 SelectmenuLightIntensity IftheR valueis 750mV checkfilterusedtime Changefilteriftheused timeis 2500hours IftheR valuestillis 750mVafte...

Page 59: ...thefilterusedtimeis 2500hours itisrecommendedtochangethefilter Lampusedtime Followthestepsbelowtocheckthelampusedtime Step Action 1 SelectmenuCheck pressOK 2 SelectmenuLampUsedTime Ifthelampusedtimeis 4000hours contactCytivaforlampreplacement Softwareversion Followthestepsbelowtocheckthesoftwareversion Step Action 1 SelectmenuCheck pressOK 2 SelectmenuSoftwareversion 5 Operation 5 9 Checkmenu Moni...

Page 60: ...ntsofthenominalflowcellpathlength thepathlengthintheUV flowcellcanbecalibrated Thisisnotnecessaryforstandardoperations ContactyourCytivarepresentativeforadvice 5 Operation 5 10 UVcellcalibration 60 MonitorUVis 920OperatingInstructions29055049AF ...

Page 61: ...mustchangeduetoasmallamountofsample beingapplied orfroma10mmto2mmwhenalowersensitivityisdesired dueto outputsignallimitation SeeSection 4 5 Installing the flow cells on page 30 Dataforup tofiveflowcellsaresavedinmemoryinMonitorUVis 920 5 Operation 5 11 Changingflowcell MonitorUVis 920OperatingInstructions29055049AF 61 ...

Page 62: ...tingtoperformanyoftheproceduresdescribedin thischapter youmustreadandunderstandallcontentsofthe Safetyinstructionschapter NOTICE ThemainspowertoMonitorUVis 920mustbeswitchedoffbefore connectingtheinstrumenttoanycellsorexternalequipment Inthischapter Section Seepage 6 1 Cleaningbeforeplannedservice 63 6 2 Cleaningtheinstrumenthousing 64 6 3 Cleaningtheflowcellandopticalconnectors 65 6 4 Storage 67 ...

Page 63: ...e Health and Safety Declaration FormortheHealth and Safety Declaration Form for Product Return or Servicing dependingonwhethertheinstrumentisgoingtobeservicedonsiteorreturnedfor service respectively Healthandsafetydeclarationforms HealthandsafetydeclarationformsareavailableforcopyingorprintingintheRefer ence informationchapterofthismanual orondigitalmediasuppliedwiththeuser documentation 6 Mainten...

Page 64: ... 1 Wipetheinstrumenthousingregularlywithadampcloth Donotallow spilledliquidtodryontheinstrument 2 Removedirtfromthesurfaceusingaclothandamildcleaningagent 3 Lettheinstrumentdrycompletelybeforeusingit 6 Maintenance 6 2 Cleaningtheinstrumenthousing 64 MonitorUVis 920OperatingInstructions29055049AF ...

Page 65: ...aticsolution e g NaOH anddistilledwater before serviceandmaintenance NOTICE KeepUVflowcellclean Donotallowsolutionscontaining dissolvedsalts proteinsorothersolidsolutestodryoutintheflow cell Donotallowparticlestoentertheflowcell asdamagetothe flowcellmayoccur Cleaningtheflowcell Followthestepsbelowtocleantheflowcell Step Action 1 Connectasyringetotheinletoftheflowcellandsquirtdistilledwater throug...

Page 66: ... Rinsethesyringeandflushthecellwithdistilledwater 10mL Cleaningtheopticalfiberconnectors Whenrequired wipetheopticalfiberconnectorswith30 isopropanolonlenspaper 6 Maintenance 6 3 Cleaningtheflowcellandopticalconnectors 66 MonitorUVis 920OperatingInstructions29055049AF ...

Page 67: ...r Overnight Theflowcellcanbeleftfilledwithbuffer Weekendandlongtermstorage Flushtheflowcellwithdistilledwaterandthenfillitwith20 ethanol Theflowcellcanalsobestoreddrybyflushingasabovewithdistilledwaterandthen blowingacompressedinertgassuchasnitrogen N2 throughthecell Replacethe protectivecaps Neverusecompressedairasthismaycontaindropletsofoil 6 Maintenance 6 4 Storage MonitorUVis 920OperatingInstr...

Page 68: ...theproblem oriftheproblemisnot coveredbythisguide contactyourCytivarepresentativeforadvice Safetyprecautions WARNING Beforeattemptingtoperformanyoftheproceduresdescribedin thischapter youmustreadandunderstandallcontentsofthe Safetyinstructionschapter Inthischapter Section Seepage 7 1 Problemsandcorrectiveactions 69 7 2 Errormessages 71 7 Troubleshooting 68 MonitorUVis 920OperatingInstructions29055...

Page 69: ... signal driftorinstability Thebuffermaybeimpure Checkwithwaterifthe signalisstillnoisy Theremaybeairinthe flowcell Ifthereisalotofairinthe water degasthebuffer continuously werecom mendheliumsparging Checktheconnectionsof theUVcellopticalfibers Dirtintheflowcellor fiberconnectors CleantheUVcell see Cleaning the flow cell on page 65 Ghostpeaks Airintheeluents Degasifnecessary we recommendhelium spa...

Page 70: ...roperly setup Checkthechartrecorder inaccordancewithits manual MonitorUVis 920not properlysetup Testtherecorderfunc tionbyselectingrecorder testaccordingtoCheck analog output on page 57 7 Troubleshooting 7 1 Problemsandcorrectiveactions 70 MonitorUVis 920OperatingInstructions29055049AF ...

Page 71: ...e light Internalerror ContactyourCytivarepresentative Lowtransmission Thetransmissionintheflowcellistoolowduetoair bubblesortoohighabsorptioninthebuffer Flushcelland orchangebuffer Lowlightintensity SeeLight intensity on page 58 Serialnonotvalid Serialnumberenteredfromdisplayisnotvalid Entera correctserialnumber Mustbe1000orhigher Error100 TheUV signalisnotstableduringcalibration See Section 7 1 P...

Page 72: ...includesorderinginformationandHealthandSafetyDeclarationformsforservice Inthischapter Section Seepage 8 1 Specifications 73 8 2 Recyclinginformation 78 8 3 Regulatoryinformation 79 8 4 Orderinginformation 88 8 5 HealthandSafetyDeclarationForm 91 8 Referenceinformation 72 MonitorUVis 920OperatingInstructions29055049AF ...

Page 73: ...tion Digitalinputs 5V 1mAcurrentsinking lampon off autozero eventmark Display 2rowswith20characterseach Dimensions HxWxD 200x160x262mmwithoutcellholder 200x300x262mmwithcellholder Weight 5 1kg Acousticnoiselevel 70dBA Enclosureprotectiveclass IP20 Environmentalrequirements Parameter Requirement Ambienttemperature 4 Cto40 C Relativehumiditytolerance non condensing 20 to95 Atmosphericpressure 84to10...

Page 74: ...calvaluesatroomtemperatureafter2hourswithlampon UVflowcell 2and10mm Parameter Specification Recommendedmaximumflowrate 100mL min Maximumpressure 2MPa 20bar 290psi Backpressure Maximum0 5barat2mL minwith waterat25 C Liquidtemperaturerange 4 Cto40 C Opticalpathlength 2mmcell 2mm Opticalpathlength 10mmcell 10mm Cellvolume 2mmcell 2μL Cellvolume 10mmcell 8μL Wettedmaterials PTFE polytetrafluoroethylen...

Page 75: ...re 2MPa 20bar 290psi Backpressure Max 0 05barat400mL minwithwater at25 C Liquidtemperaturerange 4 Cto40 C Opticalpathlength 1 2and5mm Cellvolume 300μL Wettedmaterials PEEK polyetheretherketone Titanium palladiumalloy FFKM perflorelastoner orEPDM Quartz syntheticfusedsilica pHstabilityrange 1 13 13 14 1daysexposure Chemicalresistance Thewettedpartsareresistantto organicsolventsandsaltbuffers common...

Page 76: ...exposure Chemicalresistance Thewettedpartsareresistantto organicsolventsandsaltbuffers commonlyusedinchromatographyof biomolecules except100 ethyl acetate 100 hexane and100 tetra hydrofuran THF or15 THFinacetoni trile Tubingconnections TC25connector IndustrialFlowCell1inch Parameter Specification Recommendedmaximumflowrate Maximumpressure 1MPa 10bar 145psi upto40 C Backpressure Max 0 4barat600L hw...

Page 77: ...posure Chemicalresistance Thewettedpartsareresistantto organicsolventsandsaltbuffers commonlyusedinchromatographyof biomolecules except100 ethyl acetate 100 hexane and100 tetra hydrofuran THF or15 THFinacetoni trile Tubingconnections TC50connector 8 Referenceinformation 8 1 Specifications MonitorUVis 920OperatingInstructions29055049AF 77 ...

Page 78: ...and recycledaccordingtonationalandlocalenvironmentalregulations Recyclingofhazardoussubstances Theproductcontainshazardoussubstances Detailedinformationisavailablefrom yourCytivarepresentative Disposalofelectricalcomponents Wasteelectricalandelectronicequipmentmustnotbedisposedofasunsortedmunic ipalwasteandmustbecollectedseparately Contactanauthorizedrepresentativeof themanufacturerforinformationc...

Page 79: ...page 8 3 1 Contactinformation 80 8 3 2 EuropeanUnionandEuropeanEconomicArea 81 8 3 3 EurasianEconomicUnion Евразийскийэкономическийсоюз 82 8 3 4 RegulationsforNorthAmerica 84 8 3 5 Regulatorystatements 85 8 3 6 DeclarationofHazardousSubstances DoHS 86 8 Referenceinformation 8 3 Regulatoryinformation MonitorUVis 920OperatingInstructions29055049AF 79 ...

Page 80: ... Manufacturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB Björkgatan30 SE75184Uppsala Sweden Telephonenumberofmanufacturer 46771400600 8 Referenceinformation 8 3 Regulatoryinformation 8 3 1 Contactinformation 80 MonitorUVis 920OperatingInstructions29055049AF ...

Page 81: ...fnotincludedwiththeproduct acopyoftheEUDeclarationofConformityisavailable onrequest CEmarking TheCEmarkingandthecorrespondingEUDeclarationofConformityisvalidforthe instrumentwhenitis usedaccordingtotheOperating Instructionsorusermanuals and usedinthesamestateasitwasdelivered exceptforalterationsdescribedinthe Operating Instructionsorusermanuals 8 Referenceinformation 8 3 Regulatoryinformation 8 3 ...

Page 82: ...emanufacturerand importer inaccordancewiththerequirementsoftheTechnicalRegulationsofthe CustomsUnionand or theEurasianEconomicUnion Requirement Information Name addressandtelephone numberofmanufacturer SeeManufacturing information Importerand orcompanyfor obtaininginformationabout importer CytivaRUSLLC 109004 Moscow internalcityareaTaganskymunicipal district Stanislavskystr 21 building3 premisesI ...

Page 83: ...тиваРУС 109004 городМосква вн тер г муниципальныйокруг Таганский улицаСтаниславского дом21 строение 3 помещениеI комната57 РоссийскаяФедерация Телефон 74996091550 Адресэлектроннойпочты rucis cytiva com 8 Referenceinformation 8 3 Regulatoryinformation 8 3 3 EurasianEconomicUnion Евразийскийэкономическийсоюз MonitorUVis 920OperatingInstructions29055049AF 83 ...

Page 84: ...e the equip ment ThisequipmenthasbeentestedandfoundtocomplywiththelimitsforaClassAdigital device pursuanttopart15oftheFCCRules Theselimitsaredesignedtoprovide reasonableprotectionagainstharmfulinterferencewhentheequipmentisoperatedin acommercialenvironment Thisequipmentgenerates uses andcanradiateradio frequencyenergyand ifnotinstalledandusedinaccordancewiththeinstruction manual maycauseharmfulint...

Page 85: ...onments SouthKorea RegulatoryinformationtocomplywiththeKoreantechnicalregulations NOTICE ClassAequipment equipmentforbusinessuse Thisequipmenthasbeenevaluatedforitssuitabilityforuseina businessenvironment Whenusedinaresidentialenvironment thereisaconcernofradio interference 유의사항 A급기기 업무용방송통신기자재 이기기는업무용환경에서사용할목적으로적합성평가를받 은기기 로서가정용환경에서사용하는경우전파간섭의우려가있습 니다 8 Referenceinformation 8 3 Regulatoryinformat...

Page 86: ...ctricalandelectronicproducts ThenumberinthesymbolistheEnvi ronment friendlyUsePeriod EFUP whichindicatestheperiodduringwhichthehazardous substancescontainedinelectricalandelectronicproductswillnotleakormutateundernormal operatingconditionssothattheuseofsuchelectricalandelectronicproductswillnotresultin anysevereenvironmentalpollution anybodilyinjuryordamagetoanyassets Theunitofthe periodis Year In...

Page 87: ...规定的限量要求 此表所列数据为发布时所能获得的最佳信息 0 Indicatesthatthishazardoussubstancecontainedinallofthehomogeneous materialsforthispartisbelowthelimitrequirementinGB T26572 X Indicatesthatthishazardoussubstancecontainedinatleastoneofthe homogeneousmaterialsusedforthispartisabovethelimitrequirementin GB T26572 Datalistedinthetablerepresentsbestinformationavailableatthetimeof publication 8 Referenceinformation 8 3 Reg...

Page 88: ...ct code UVFlowCell2mm includingopticalfibres 1 18111110 UVFlowCell10mm includingopticalfibres 1 18111111 UVFlowCellÄKTApilot1 2 5 including opticalfibres 1 11000850 IndustrialFlowCell8mm 1 18113456 UVFlowCell1 PEEKFPM FKM 1 28959578 IndustrialflowcellshortopticalfibreKit 20 cm 1 18113485 IndustrialflowcellshortopticalfibreKit 50 cm 1 18113486 Filterunits Item Quantityper pack Product code 215nm 1 ...

Page 89: ...0 18111249 Ferrulefor3 16 tubing 10 18111248 Stopplug 5 16 5 18111250 Stopplug 1 16 5 18111252 UnionLuerfemale 1 16 male 2 18111251 Union1 16 female M6male 6 18111257 UnionM6female 1 16 male 8 18111258 Union1 16 male 1 16 male i d 0 25mm 2 18112092 Union1 16 male 1 16 male i d 0 50mm 2 18112093 PEEKtubing i d 0 15mm o d 1 16 2m 18115659 PEEKtubing i d 0 25mm o d 1 16 2m 18112095 PEEKtubing i d 0 5...

Page 90: ... 16 2m 18112136 ETFEtubing i d 0 75mm o d 1 16 2m 18111254 Fingertightconnector1 16 10 18111255 Tools Item Quantityper pack Product code Fiberdetachmenttool 1 18111116 8 Referenceinformation 8 4 Orderinginformation 90 MonitorUVis 920OperatingInstructions29055049AF ...

Page 91: ... some cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have been removed or isolated from the instrument and from any area that may impede access to the instrument All buffer waste vessels are labeled Excess containers have been removed from the area to provide access Provide explanation for any No answers ...

Page 92: ... must be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other specify Equipment type Product No Serial No I hereby confirm that the equipment specified above has been cleaned to remove...

Page 93: ...Pageintentionallyleftblank ...

Page 94: ... DeconexisatrademarkofBorerChemieAG Allotherthird partytrademarksarethepropertyoftheirrespectiveowners 2020 2021Cytiva AllgoodsandservicesaresoldsubjecttothetermsandconditionsofsaleofthesupplyingcompanyoperatingwithintheCytiva business Acopyofthosetermsandconditionsisavailableonrequest ContactyourlocalCytivarepresentativeforthemostcurrent information Forlocalofficecontactinformation visitcytiva co...

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