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Regenova

®

A Bio 3D Printer

Start up Manual 

Ver.3

Feb.2016

●

This Manual is subject to change without notice.  

●

Information is as of Feb, 2016.

Cyfuse Biomedical K.K 

ICN Building-5A 2-27-17, Hongo, Bunkyo-ku, Tokyo, 113-0033, JAPAN

TEL

：

+81-3-4455-7872  E-mail

：

[email protected]

URL

：

http://www.cyfusebio.com

1

Summary of Contents for Regenova

Page 1: ...3 Feb 2016 This Manual is subject to change without notice Information is as of Feb 2016 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sa...

Page 2: ...4 4 Setting up Cell Accumu 16 5 3D printing 20 6 After 3D printing 25 Maturation 1 Maturation 27 2 Removing tissue construct from Kenzan 28 FAQ 29 Advanced Operation 31 Cyfuse Biomedical K K ICN Build...

Page 3: ...e for 9X9 Kenzan CY0 104 00 1 1 500 Kenzan Base Plate for 26X26 Kenzan CY0 105 00 1 1 500 Kenzan Container CY0 103 00 1 500 Nozzle 25G CY0 107 00 60 units 250 Nozzle 26G CY0 102 00 5 Nozzle 27G CY0 10...

Page 4: ...uantity 676 needles 26 x 26 Material Stainless Steel Heat resistant resin Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm co...

Page 5: ...mmended for regular spheroids Recommended single usage due to potential clogging Kenzan Base Plate For 9X9 CY0 104 00 For 26X26 CY0 105 00 Kenzan Container CY0 103 00 Air System CY0 101 00 Waste Bin C...

Page 6: ...ulture enough cells for your 3D printing project It is recommended to use cells in near a log growth phase 6 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3...

Page 7: ...es cyfusebm com URL http www cyfusebio com Prior to 3D Printing 3 Designing your 3D structure 7 For more details please refer to the manual of B3D software Designs recommended for beginners Tube Use 9...

Page 8: ...ding 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Prior to 3D Printing 4 Sterilizing Accessories and Tools Before sterilizati...

Page 9: ...N TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Prior to 3D Printing 4 Sterilizing Accessories and Tools For the nozzle and Kenzan it is recommended to prepare and autoclave...

Page 10: ...it is recommended to sterilize them together in the following manner Air bubbles can disrupt camera images and cause failure to detect needle tips Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo B...

Page 11: ...al K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com 3D Printing 1 Powering on 1 START 2 STOP 3 ALARM STOP 4 ABNO...

Page 12: ...ion power supply to ON and wait a minute until it is fully initialized 5 Power the PC on Stop alarm with ALARM STOP Main power operation power supply ON OFF Cyfuse Biomedical K K ICN Building 5A 2 27...

Page 13: ...uidlines for Regenova The sash of the clean bench must be closed down to the bottom When an alarm is activated press ALARM STOP and ABNORMAL RESET Then operation can resume Buttons on the right tab of...

Page 14: ...der Nozzle Holder Kenzan Container stage Air Tube Step Place the Waste Bin Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm c...

Page 15: ...sure to avoid touching the tip of the nozzle to keep it clean 3 Tighten the screw at the nozzle stand Step 6 Close the front panel of the clean bench down to the bottom and hold down Home Return 15 3...

Page 16: ...Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Step 3 Inspecting Kenzan Make sure Home Return is pink If not hold down the button until it...

Page 17: ...low Press total then the system will calculate how many layers are possible to us for the print Then press Apply Step 6 Adjust z direction interval 17 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hon...

Page 18: ...checks again to confirm successful pick up Press Inspection Parameters Select Type 1 or Type 2 tab Adjust set parameters or previous data can be inserted from File Parameters on Type 1 tab should be b...

Page 19: ...dle Array Map page and adjust Ring Light of upper camera Press Apply to activate Recommended settings of lights for spot and ring light are as follows 9X9 standard around 100 9X9 tube around 80 26X26...

Page 20: ...m 3D Printing 5 3D Printing 1 In the Needle Array Map page place 3 4 spheroids on the top layer 2 Press and hold START 3D printing only for those few spheroids 3 Press and hold STOP 3D printing after...

Page 21: ...r with following steps Adjust diameter Uncheck capture image Press Execute Set Diameter to cover the diameter of the nozzle Adjust Threshold Uncheck Auto set Increase Threshold 10 Threshold column wil...

Page 22: ...stop the alarm 3 Cover the Kenzan container to avoid contamination 4 Replace the current nozzle with a new one 5 Remove the cover from the Kenzan container and close the sash 6 Hold down Home Return a...

Page 23: ...se Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com 3D Printing 5 3D Printing Troubleshooting 3 No or...

Page 24: ...1 needle 1 and well 2 to start can be selected 24 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio...

Page 25: ...OT2000 and then shut the PC down 25 To save all data File Save Experimental Parameters All This data can now be used for future printing by loading it from File Cyfuse Biomedical K K ICN Building 5A 2...

Page 26: ...Turn off Operation power supply with the key ON OFF 1 Open the air bleed valve of the compressor by turning counter clockwise and drain water Typically Main Power remains ON Step 7 Cleaning Wash all a...

Page 27: ...n Chamber Type 1 CY1 004 00 is strongly suggested Please refer to the following directions for using the Perfusion Chamber Type 1 27 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo...

Page 28: ...ce grid plate 2 on grid plate 1 Move both plates down to Kenzan base Maturation 2 Removing tissue construct from Kenzan Remove the construct after maturation on Kenzan Re insert the grid plate which w...

Page 29: ...fter Needle Array Check alarm Check the selection of Kenzan Type page to ensure the correct sized Kenzan is chosen Stack S1 Bubbles are observed each time spheroids are skewered Check the Air System c...

Page 30: ...not to contaminate the nozzle S13 Skewering to the wrong needle or place Press EMERGENCY STOP immediately There are several reasons for this Bubbles in the Kenzan Remove bubbles with pipette and see...

Page 31: ...mirror to see the display Unlock You will only need to unlock once each time the machine is turned on 1 Hold down key until Loc on the display starts to blink 2 Press S Key firmly to unlock the displa...

Page 32: ...ic needle to penetrate Press Setting 2 and point to the tip of the desired needle Then press Setting 2 again and press Apply 3 Then the nozzle will pick up and penetrate the spheroid onto the needle 3...

Page 33: ......

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