PN-010046 REV G
21 September 2017
PRODUCT INSERT
:
tissueTUBEs and Plugs (TT1, TT1-XT and TT1-P)
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain an infectious agent.
Laboratories handling specimen samples are advised to comply with applicable parts of the following governmental and clinical standards, or their
equivalent in the country of use:
•
Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other Blood borne Infections, published 1987, updated
1996
•
Clinical and Laboratory Standards Institute (CLSI), GP17-A3 Clinical Laboratory Safety; Approved Guideline - Third Edition, published 2012, ISBN 1-56238-
797-9
•
Clinical and Laboratory Standards Institute (CLSI), M29-A4 Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline,
Fourth Edition, published 2014, ISBN 1-56238-961-0
•
Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Blood borne Pathogens
•
International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety
CRYOGENIC HAZARD
The
cryoPREP
system requires the use of low temperature materials and equipment, including -80°C freezers, dry ice, and/or liquid nitrogen.
Personnel must be trained to safely handle these materials and associated equipment.
Storage Conditions Prior to Use
TT1 and TT1-XT tissueTUBEs and tissueTUBE Plugs (TT1-P) may be stored at room temperature until employed and are stable for at least one
year under these conditions. There is an expiration date on all packaging.
Limitations on in vitro Usage
TT1, TT1-XT, and TT1-P are developed, designed, and sold for research use only. They are not to be used for human diagnostic purposes or
treatment unless expressly cleared for that purpose by the Food and Drug Administration in the USA or the appropriate regulatory authorities in the
country of use.
Product Warranty Guarantee
Covaris guarantees the performance of all products when used in accordance with our written instruction, under normal operating conditions, and
during the expiration period. The user must determine the suitability of the product for its particular use. Should any product fail to perform
satisfactorily due to any other reason than misuse, Covaris will replace it free of charge. We reserve the right to change, alter, or modify any product
to enhance its performance or design. If a product does not meet your expectations, please contact Covaris Technical Assistance.
Technical Assistance
On-going assistance with the operation or application of any of our products is provided via:
•
Telephone during the hours of 9AM to 5PM, Monday through Friday, (GMT-05:00) Eastern Time (US & Canada) +1 781 932 3959
•
E-mail queries to
INSTRUCTIONS FOR USE
(Please refer to the
cryoPREP
User Manual for further instructions on use of this instrument)
TissueTUBEs will be referred to as TT1 for the remainder of this insert.
Purpose
The cryoPREP system employs a programmable impact hammer to pulverize cryogenically frozen tissue samples, employing the brittleness of
frozen biological samples for reproducible pulverization. The process increases sample surface area and breaks extra-cellular matrices. The TT1
tube and plug are designed to allow easy transfer of the pulverized material to a 16mm diameter tissue vial. During subsequent treatment in Covaris
AFA instruments such as the S-Series and E-Series, homogenization times are reduced and the extraction efficiency of target bio-molecules
improved, enabling larger sample size and higher throughput.
Equipment Notes
The TT1 tissueTUBE is designed to be used with the Covaris cryoPREP CP02 instrument. The CP02 is available in either a 100-120 volt model
(PN 500001) for use in the Americas or Japan or in a 200-240 volt model (PN 500000) for use in Europe, Asia and Australia.
The CP02 must be equipped with a TT1 Holder (Covaris PN 500095) to accept TT1 sample tubes.
The TT1 is designed for use with biological tissue samples of 100 to 1000 milligram mass. Typical samples range from 250mg to 500mg.
Appropriate tissues to use with the TT1 include liver, kidney, skeletal muscle, heart, dermal, lung, brain, and adipose.
The TT1-XT is a thicker walled tube designed to withstand greater stress. The TT1-XT is recommended for hard tissue such as: bone, seeds, plant
material, and tablets. Cell culture pellets are not appropriate for use in either version of TT1 tube. Do not exceed 1000mg of mass.
The TT1 employs a 15-415 threaded top to mate with a standard 16x100 mm culture tube. Covaris recommends a disposable, borosilicate, round
bottom glass tube with polypropylene cap (Covaris PN 520011, Fisher Scientific PN 14-962-26F or VWR PN 60825-837). For applications that
employ other tubes sizes, please contact Covaris.