Covaris microTUBE-500, Manual, Page: 1 / 4

1 Part Number: 010526 Rev A
Covaris
June 2020
Introduction
The microTUBE-500 AFA Fiber Screw-Cap ( PN 520185 ) is a singular vessel designed to hold up to 500 µl of
sample for sample processing. Each tube contains an AFA fiber that improves reproducibility, promotes an
isothermal process, reduces fragmenting times, and allows larger sample volumes to be processed.
The microTUBE-500s have been validated for DNA shearing at volumes of 320 µl and 500 µl. They are
compatible with the M-series, S220, E-series and L-Series Covaris instruments and require the use of an
instrument specific Rack or Holder. Refer to the Covaris-certified Consumable Guide for further information
( covaris.com/wp-content/uploads/M020065.pdf ). DNA shearing protocols with the microTUBE-500 are also
instrument specific. Do not store sheared samples in the microTUBEs.
The microTUBE-500 has a 2D barcode which can be used for sample traceability.
The microTUBE-500s are also a part of the truXTRAC
®
FFPE Plus product line and included in the following kits: PN 520255, PN
520252, PN 520262, and PN 520271. Please refer to the Nucleic Acid Extraction from FFPE Protocols: ( covaris.com/resources/
protocols/nucleic-acid-extraction-from-ffpe/ ).
Recommended instructions are subject to change without notice.
Ordering Information
• microTUBE-500 AFA Fiber Screw-Cap (25) ( PN 520186 )
• microTUBE-500 Screw-Cap Prep Station ( PN 500510 )
• microTUBE-500 Centrifuge and Heat Block Adapter (10) ( PN 500503 )
DNA Shearing Protocols
• Quick Guide: DNA Shearing with M220 ( https://covaris.com/wp-content/uploads/pn_010252.pdf )
• Quick Guide: DNA Shearing with ME220 ( https://covaris.com/wp-content/uploads/pn_010349.pdf )
• Quick Guide: DNA Shearing with S220 ( https://covaris.com/wp-content/uploads/pn_010368.pdf )
• Quick Guide: DNA Shearing with E220 ( www.covaris.com/wp-content/uploads/pn_010308.pdf )
• Quick Guide: DNA Shearing with LE220 ( www.covaris.com/wp-content/uploads/pn_010156.pdf )
• Quick Guide: DNA Shearing with LE220-plus/R-plus/Rsc ( www.covaris.com/wp-content/uploads/pn_010433.pdf )
Product Insert: microTUBE-500 AFA Fiber Screw-Cap