Cole Parmer 81150-30 Operating Instructions Download Page 1

Operating Instructions

 

Handheld Analog Refractometers

Operation

Preparation. Hold the refractometer in a horizontal position. Aim the front end of the 

refractometer in the direction of a bright light, and use the adjusting ring of diopter 

until the reticle can be seen clearly.

Zero % adjust with distilled water. Place a few drops of pure water on the prism  

surface and close the prism cover. If bubbles form, gently pressing the cover will 

remove the bubbles and help disperse the water over the entire surface. 

Hold the refractometer up to natural light or an incandescent bulb to obtain the  

reading. Looking into the eyepiece, you should see a distinct separation between a 

blue and white section, often called a “contrast” line. If the contrast line is not directly 

at zero, then adjust by turning the calibration screw on the top of the refractometer 

until it reads zero. Replace the plastic cap after adjusting the screw to prevent water 

from entering the refractometer. 

Adjust the focus by twisting the eyepiece until the scale can be seen clearly. Once the  

refractometer is calibrated to zero with pure water, dry the surfaces with a clean cloth. 

–  For models with 28% to 62% Brix range: Open cover plate, drop one or two drops of  

saturated sodium chloride (table salt) solution on the prism surface, close cover 

plate. Adjust to 29.9% at 15°C, 29.6% at 20°C, 29.2% at 25°C.

–  For ATC models with 58 to 92% Brix range: Owing to the remarkably consistent 

properties of extra-virgin olive oil, one drop of it on the slide will always read 

between 71% and 72% on the Brix scale. If you set the calibration screw to show any 

such oil at 71.5%, you will have correctly calibrated the water content scale at the 

same time.  

–  For models with multiple Brix scales: These units have been adjusted at the factory. 

To use multiple scales, rotate the adjusting wheel (model 81150-30) or adjusting knob 

(model 81150-33) to change scales. To reduce chromatism, rotate the adjusting ring.

Measurement. Open the cover plate, clean the surface of prism with a piece of soft 

paper or cotton, then drop 1 to 2 drops of the solution to be measured. Close the 

cover plate, and press it lightly, then read the corresponding scale of light and dark 

boundary. The reading is the Brix, salinity concentration, or refractive index (RI) of 

measured solution.

Storage. After measurement, clean the surface of prism and cover plate with moist 

gauze, and store carefully. Do not immerse or dip in water so that water does not 

enter the inside of the unit.

Refractometers with Automatic Temperature Compensation (ATC)

The reference of temperature is 20°C. In operation, the temperature should be made 

according to the Correction Table below. The automatic temperature compensation 

(ATC) function enables users to concentrate on measurements without worrying about 

the temperature. Compensation range is 10 to 30°C.

Abbreviated International Temperature Correction Table for a Refractometer Tested at 20°C

Temperature

Dry substance content in percent

°C

°F

0

5

10

15

20

10

50

Substract 0.50 Brix

Substract 0.54 Brix

Substract 0.58 Brix

Substract 0.61 Brix

Substract 0.64 Brix

15

59

Substract 0.27 Brix

Substract 0.29 Brix

Substract 0.31 Brix

Substract 0.33 Brix

Substract 0.34 Brix

20

68

None required

25

77

Add 0.33 Brix

Add 0.35 Brix

Add 0.36 Brix

Add 0.37 Brix

Add 0.38 Brix

30

86

Add 0.72 Brix

Add 0.74 Brix

Add 0.77 Brix

Add 0.78 Brix

Add 0.79 Brix

Description

1) Prism
2) Cover plate
3) Calibration screw
4) Mirror tube
5) Eyepiece (adjusting ring of diopter)

Accessories (not shown)

Eyeshade
Suction tube
Screwdriver

Adjusting wheel

Adjusting ring

Adjusting knob

Model 81150-30

Model 81150-33

Zero calibration

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